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      • 간호 학생의 임상실습 만족도와 교수 효율성과의 관계 연구

        김순옥 順天靑巖大學 1997 論文集-順天靑巖大學 Vol.21 No.1

        The purpose of this study is to contribute to the development of clinical instruction by finding out students ratings of the Satisfaction Degree after the clinical practice and the Teaching Effectiveness in clinical nursing education. The subject consisted of 287 Nurse Students in the second and third Junior College in Chonla Nam Do. These data were collected by conducting questionaires during 20 days Form November 12 to December 2, 1996. The questionaire consisted of four areas as follows : The General Characteristics and Status of Clinical Nursing Education which was composed of 14 items the satisfaction Degree after the clinical practice which was composed of 12 items, the Uneasy feeling after the clinical practice which was composed of 15 items, and the Teaching Effectiveness which was composed of 31 items. The collected data were analyzed by the actual number, its percentage, the mean score, standard deviation, t-Test, ANOVA, pearson correlation coefficient. The result of this study were as follows : 1. 58.9% of nursing students felt that their clinical teachers played a role model, the reason was based on the fact that they belive the clinical teachers had professional knowledge and clinical experience(33.1%). However, the judgement was futher based on how well the teachers applied their knowledge and experience. responded a team of nursing professor and head nurse and graduate nurse. The reason was that they felt clinical teachers are able to connect theory with practice. 3. Student felt that the quaslification for clinical teachers should be at least a master's degree holder and 5 or more years of clinical experience. The reason was that they felt knowledge and experience were imperative for professional education. 4. 80.7% of nursing students responded that lectures and clinical practice must be taken together. The content for clinical education comprised of Orientation being the most frequence of 45.2% : Nursing Process 20.8% : Basic Nursing Skills 13.8% : Pathology 8.1% : Inspection(making rounds)5.3% :Conference 3.9% and other Nursing Skills of 2.8% being the least frequence. 5. The mean score for Teaching Effectiveness was 3.157(±0.540). 6. The mean score for the Uneasy Feeling after the clinical practice was 3.042(±0.556). 7. The mean score for the Satisfaction Degree after the clinical practice was 3.659(±0.597). 8. The result of the correlation between of the Teaching Effectiveness, the Uneasy feeling and Satisfaction Degree after the clinical practice : The higher the Teaching Effectiveness, the higher Satisfaction Degree after the clinical practice(r=0.333, p=0.0001). The higher the Uneasy Feeling after the clinical practice, the lower Satisfaction Degree after the clinical practice(r=0.173, p=.005). 9. The students who made the selection of nursing department for themselves expressed lower Uneasy Feeling after the clinical practice(t=-3.601, p=.0004) and higher Satisfaction Degree after the clinical practice(t=3.955, p=.0002). 10. the students, whose clinical teachers was role model expressed higher Satisfaction Degree after the clinical practices(t=3.755, p=.000). Other variables, did not significant difference.

      • KCI등재
      • 발생중인 흰 쥐 뇌하수체의 Acetylcholinesterase에 대한 연구

        김순옥,우재형 고신대학교(의대) 고신대학교 의과대학 학술지 1988 고신대학교 의과대학 학술지 Vol.4 No.1

        Experiments were made to determine the presence of cholinergic biomarker, acetylcholinesterase (AChE) in the rat hypophysis of development. A histochemical procedure for AChE was used to provide visualization of structure containg this enzyme. AChE activity of developing rat hypophysis first demonstrated in 16 days and then the more increased activity was found in 19 and 20 days. Cells staining for AChE were found in the pars distalis and pars intermedia of the developing rat hypophysis. Nerve fibers staining for AchE activity were observed in the pars nervosa. The presence of AChE and choling acetyltransferase(ChAT) (Sarah and Donald, 1983) in the pars intermedia and the pars nervosa is evidence for a cholinergic innervation to these regions. In te pars distalis, where other investigators have found muscarinic receptors, intense staining for AChE and absence of ChAT activity (Sarah and Donald, 1983) may indicate non-innervated, acetylcholine-sensitive sites.

      • SCOPUSKCI등재

        Streptomyces lividans TK24에서 secY homolog의 클로닝과 분석

        김순옥,서주원 한국산업미생물학회 1998 한국미생물·생명공학회지 Vol.26 No.2

        몇가지 그람양성 세균에서 secY 유전자가 함유된 operon의 구성이 rplO(L15)-secY-adk라는 결과를 토대로 L15와 adk 유전자일부를 primer로 제작하여 Streplomyces lividans TK24에서 secY 유전자가 함유된 1.8kb 단편을 PCR로 증폭하여 얻은 후 secY 유전자를 cloning하였다. 전 단편을 sequencing하여 추론한 아미노산으로 상동성을 조사해 본 결과 Escherichia coli, Bacillus subtilis, Micrococcus luteus, Bacillus licheniformis, Staphylococcus carnosus, Brevibacterium flavum, Streptomyces scabies의 SecY와 각각 46%, 43%, 57%, 44%, 42%, 56%, 90%의 유사성을 보이는 것으로 나타났으며 SecY의 소수성 profile 또한 서로 유사하고 10개의 membrane spanning segment를 동일하게 가지는 것으로 나타났다. 유전자 구조도 다른 그람양성균에서와 같이 L15-SecY-Adk 순 이였으며 secY 유전자의 종결코돈과 adk 유전자의 개시코돈이 한 염기를 공유하는 상태의 translational coupling 구조를 하고 있었다. 이와같이 단백질분비에 관여하는 유전자와 ribosome 구성요소, 그리고 ATP 합성에 관여하는 요소는 세포성장에 상호 연관 작용이 있는 것으로 사료된다. The secY gene of Streptomyces lividans TK24 was cloned by the PCR method with synthetic oligonucleotide primers designed on the basis of the conserved regions of L15-secY-adk operon from E. coli, B. subtilis, and M. luteus. The deduced amino acid sequences of the SecY are highly homologous to those of other known SecY. It has 46%, 43%, 57%, 44%, 42%, 56%, 90% similarity to Escherichia coli, Bacillus subtilis, Micrococcus luteus, Bacillus licheniformis, Staphylococcus carnosus, Brevibacterium flavum, Streptomyces scabies, respectively and almost the same with Streptomyces coelicolor. The gene organization of L15-SecY-Adk is also similar to those of other bacteria. SecY and Adk are very likely translationally coupled that is overlapping stop codon of SecY and start codon of Adk with one base pair, which is common structure among high GC content strains of gram positive bacteria.

      • SCOPUSKCI등재

        Streptomyces lividans에서 secE 유전자의 클로닝과 염기서열 결정

        김순옥,서주원 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.3

        Streptomyces lividans TK24에서 secE 유전자를 PCR로 cloning하였다. PCR primer는 보고된 여러 방선균의 secE 유전자가 함유된 operon 내의 secE 상류부위와 nusG 하류부위 rplK N-말단을 primer로 디자인하여 Streptomyces coelicolor와 sequence를 기준으로 합성하였다. 제한효소 mapping후 500 bp의 단편을 sequencing한 결과 secE 유전자임을 확인하였으며 E. coli, B. subtilis, S. griseus, S. virginiae의 SecE와는 각각 36.8%, 30.4%, 80.0%, 80.9%의 상동성을 보였으며 S. coelicolor와는 동일한 것으로 나타났다. 이는 단백질분비 기구에 관련된 유전자들이 진화적인 차이에도 불구하고 여러 eubacteria간에 유전적으로 상당히 보존적인 것으로 보인다. 또한 합성한 primer로 증폭되고 양 말단 sequencing 결과로 보아 유전자구성도 이미 보고된 여러 방선균에서처럼 secE-nusG-rplK로 이루어져 있는 것으로 추정된다. The secE gene of Streptomyces lividans TK24 was cloned by the polymerase chain reaction method with synthetic oligonucleotide primers designed on the basis of the nucleotide sequences of Streptomyces coelicolor secE-nusG-rplK operon. The deduced amino acid sequences of the SecE were highly homologous to those of other known SecE protein, that is 36.8%, 30.4%, 80.0%, and 80.9%, similarity to E. coli, Bacillus subtilis, Streptomyces griseus, Streptomyces virginiae SecE, respectively and exactly same with Streptomyces coelicolor SecE. It means that in spite of evolutionary differences, the genes for protein translocation machinery are highly conserved in eubacteria. The gene organization of secE-nusG-rplK is also similar to that of E. coli, B, subtilis, and streptomycetes.

      • 디카르복시산 및 히드록시다카르 복시산류의 Tc-표지반응

        金純玉 誠信女子大學校 1981 硏究論文集 Vol.14 No.-

        디카르복시산 및 히드록시다카르복시산류의 ??Tc 표지 방사성의약품을 개발하기 위하여 SnCl₂를 ??Tc??의 환원제로 사용해서 citric acid, succinic acid, maleic acid, fumaric acid등을 실온下에서 ??Tc와 반응시켰다. 생성물 분리확인을 위하여는 85%메탄올과 0.85% NaCl용액을 각각 전개용매로 하는 종이크로마토그래피(PC)와 순간 얇은층 크로마토그래피(ITLC)를 실시하여 분리효과를 대비하였다. ITLC가 PC보다 분리효과면에서 우수하였고 산성pH에서의 표지수율이 알카리성에서 보다 좋았다. ??Tc착화학물 생성 수율이 높은 순위는 citric acid-tartaric acid-succinic acid-maleic acid-fumaric acid이었으며 이것은 착화제의 기능기 수나 구조적특성과 잘 일치하였다. To develope organ imaging radiopharmaceuticals, citric acid, tartaric acid, succinic acid, maleic acid, fumaric acid were separately labelled with ??Tc at room temperature using stannous chloride as a reducing agent for ??Tc??. 85% methanol and 0.85% sodium chloride were respectively used as developing solutions in both of paper (PC)-and instant thin layer(ITLC) chromatography. ITLC showed better resolutions than PC. Labelling yields were generally higher in acidic pH. The order of showing higher labelling yield, citric-tartaric-succinin-maleic-fumaric, is well explained with the number of polar functional groups and spatial characteristics of the chelating agents.

      • 一部 看護 專門大學生의 特性과 態度에 對한 調査硏究

        김순옥 순천청암대학 1984 論文集-順川看護專門大學 Vol.10 No.-

        The purpose of this study is to Suggest the improved principles of guidanse and curriculum for nursing education by surveying the veconiyations and attitudes of students of nursing School. This study was perbormed from Octover 20 to December 20, 1984, and 3p 9 students were given fuestionaire. Among them, 270 students gave the answers (They are Composed of 108 freshmen, 83 Sophomore, 79 Zinior) from this survey, we can draw following results. 1. ① The person who made the final decision to enter the nursing School was; parents 42.9%, the student herself 35.6%, brothers and sisters 8%, friends 4.1 % , Teacher 3.7%. ② The major mative of entering the nursing School was; too low grades to enter the other college and university 28%, facilty of employment after graduation 25.9%. 2. ① Level of Content with their maior was;highly and faily satisfied 56% dissatisfied and wished to transfer to other college 10.7% ② The reason of discontentment was; inadptability 38.8%, poor reputation in society 5.4% 3. ① The stndents conception of nursing profession was; ·professeion which need the high responsibility 30%, ·profession contributable to the welfare of neighbors and mankind 18.3%, ·profession which need the understanding and judgement 17.9 %, ② 47.8% of students thought that serviceship is the necessary to by nurse 4. ① Hopeful field of employment was after graduation; In hospital 54.8%, Nursing teacher 11.9%, to go abroad 8.1%, miltary nurse 7.4%, To continue to study 6.7%, nursing administrator 4.4%, ② 51.1% of students answered they want to engage the nursing profession for their lifetime 5. ① The problem whish students faced or were concerned about in School was; employment after graduation 23.3%, Getting a good grade in school 23.2%, problem of character 12.6%, problem of friendship 10.9%, problem of health 8.1%, problem of Relation with male 5.4% ② 37.8% of students wanted to engage the calture and learning as extra curricula activities. ③ When the personal problems occured the person whom students depend upon as a counseloR was;friend 33.3%, Specific professor 18.9%. ④ 30.7% of students answered they made the best effort to acfuiring the acknoulegements and technifues 6. Almost all of students(78.1%) felt that basic science course shonld be contained in nursing courses And they stated it as a reason because it is necessary to perform the nursing in future. 7. ① 75.9 % of students had experienced some problem during clinical practise and they state the reason as follows; shortage of equipments 27.2%, Conflist from differense between theory and practise 22% ② To make the matters worse, Some students(19.8%) felt the were mobilited to supplement the personels in hospital ③ And more than 50% of students thought elder nurse as a counselor when they met the problems in hospital

      • KCI등재

        유아기 아동의 효과적인 가창지도 방법 연구

        김순옥 민족음악학회 2001 음악과 민족 Vol.22 No.-

        The purpose of this research is to examinate the effectiveness of two instructional methods of singing; whole-song method and phrase-by-phrase method. A survey has conducted 117 kindergarten teachers in Busan area concerning their attitudes towards music education, teaching method related to songs. The result of this survey, 77.78% of respondents placed their emphasis on teaching songs. Therefore using an effective teaching method related to songs is very vital to achieving the stated objective. Thirty-six five-old children from two classes in a kindergarten school participated in the experiment in which they were taught two children's songs using were made by children taught by the whole-song method than when taught through the phrase-by-phrase method for both songs. More specifically, the percentage errors was significantly higher for the last three phrases of the song than the first phrase when using the phrase-by-phrase method. On the other hand, lower and even errors were made across all phrase of the songs when children were taught by the whole-song method. For the all categories of errors (pitch, rhythm, and text), performance errors were more frequently found in the phrase-by-phrase method than the whole-song method and the greatest difference was for pitch. The result of this study imply that melodic and textual fragmentation of the traditional phrase-by-phrase method may give difficulty to children in recalling and performing of songs by working against the preservation of the musical flow of the songs. The whole-song method, on the contrary, can be a more effective way of song acquisition since the continuity and integrity of the song is more easily transmitted to children. It is recommended that music teachers who stick to the traditional phrase-by-phrase method need to be aware of children's difficulty in recalling from the method and improve their instructional procedures of singing by using different procedures such as the whole-song method.

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