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해산어 종묘 생산 시기에 발생하는 바이러스성 신경괴사증 ( VNN ) 원인바이러스의 유전학적 비교
김석렬(Suk Ryol Kim),정성주(Sung Ju Jung),김영진(Young Jin Kim),김진도(Jin Do Kim),정태성(Tae Sung Jung),최태진(Tae Jin Choi),오명주(Myung Joo Oh),길수수(Mamoru Yoshimizu) 한국수산과학회 2002 한국수산과학회지 Vol.35 No.3
N/A This study was performed both to explore the host of nervous necrosis virus (NNV) between mariculturing fish species and to examine the phylogenic position of the NNV in Korea. NNV was confirmed on the basis of histopatbological and molecular biological examination, then VNN infection was proved from either moribund or dead fishes including red drum, Sciaenops ocellatus; oblong rock fish, Sebastes oblongus and flounder, Paralichthys olivaceus. As a result of sequencing for a part of NNVs, virus from red drum was showed 98%, 97%, 86% and 74% homology with oblong rock fish, grouper, Japanese flounder and striped jack, respectively. On the other hand, NNV from oblong rock fish was demonstrated 96%, 85% and 72% homology with grouper, Japanese flounder and striped jack, respectively. NNV from red drum and oblong rock fish was exhibited phylogenically distant from the representative NNV, SJNNV originated from striped jack. On the contrary, the viruses appeared to be similar species with Taiwan NNV isolated from culturing grouper.
오명주,길수수 한국어병학회 1996 한국어병학회지 Vol.9 No.2
연어과 어류의 이상유영 원인 바이러스 RVS의 ELISA법에 의한 신속 진단 방법을 개발하였다. 주화세포를 이용한 바이러스 배양액 및 감염 무지개송어의 뇌조직 마쇄액을 사용하여 실험하였다. 바이러스 배양액을 이용한 ELISA법의 검출 감도 조사에서 최소 바이러스 감염가 검출 한계치는 10^(2.6) TCID_(50)/100 ㎕ 이었다. 또한, 인공감염어의 뇌조직 마쇄액 내의 RVS 항원도 검출 되었다. 본 방법은 현장에서의 RVS 감염어 조사에 효과적으로 사용되어질 수 있는 방법으로 생각 되어진다. An indirect double antibody enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of a new virus isolated from abnormally swimming salmonid fish, RVS (Retrovirus of salmonid). Results using brain tissue homogenates, and infected cell cultures are described. The sensitivity of the methods is 10^(2.6) TCID_(50)/100 ㎕ of the examined cell culture fluid. The specificity was confirmed by the ELISA inhibition test and virological examinations. Viral antigen could be detected in artificially infected fish tissue homogenates. The assay will allow the diagnosis of RVS-infected fish within a day.