http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 길수수 (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
-
吉水守,日向進一,吳明柱,生駒三奈子,木村喬久,森立成,野村哲一,繪面良男 한국어병학회 1993 한국어병학회지 Vol.6 No.2
On the development of hirame(Paratichtys olivaceus) culture, outbreak of scuticociliata infection was reported to cause severe damage in Japan. To establish effective measures for isolation and cultivation of this ciliate, we tried to culture this pathogenic ciliate using medium for bacteria and fish cell lines in vitro. Scuticociliata from the brain tissues of infected fish was aseptically inoculated to CHSE-214 cells cultured in MEM-10 without antibiotic. Scuticociliata grew well and the number of ciliate reached 106 cells/㎖ at temperatures of 15℃ to 20℃ for 10d. The number of ciliate cultured in the cell lines is 10 times higher than the numbers cultured in the liquid medium alone. This ciliata could be cloned by dilution method. Scuticociliata isolated could grow well on 42 different cell lines that were established from marine fish, warm freshwater fish, and salmonids. This ciliate could be preserved in liquid nitrogen for more than 6 months. Subsequently, we observed the optimal temperature and salinity for growth, and tested the sensitivities of this organism to formaldehyde, flagyl(Metronidazole), Ekuteshin(Combination compound of sulfamonometoxin and ormethoprim), and ozonixation. Optimal temperature for growth was 25℃ and salinity was 1.0 to 1.5%. Washed scuticociliata was killed by formaldehyde at the concentration of 50ppm for 10min, but was not completely killed even at a high concentration of 400ppm for 20min in MEM-5. Flagyl and Ekuteshin can inhibit the growth of scuticociliata at the concentration of 1,000 and 100㎍/㎖ in MEM-10, respectively. More than 99% of this scuticociliata could be killed by ozonization at a dose equivalent to 1.0㎎/ℓ oxidant for 30sec in sea water. Isolated scuticociliata showed the pathogenicity to the cultured hirame by artificial infection(I. P. injection, 10^5 cells/fish). The number of scuticociliata in the water could be counted by most probable number(MPN) method using tissue culture, and the minimum detectable number was 1.8cells/ℓ. The number in the reservoir tank for water supply to the culture tank was 110cells/ℓ. After cleaning by elimination of the sediments from of the reservoir tank and disinfected with formaldehyde, number of scuticociliata decreased and was counted less than 1.8cells/ℓ and infection rate of cultured hirame was decreased.
-
오명주,길수수 한국어병학회 1996 한국어병학회지 Vol.9 No.2
연어과 어류의 이상유영 원인 바이러스 RVS의 ELISA법에 의한 신속 진단 방법을 개발하였다. 주화세포를 이용한 바이러스 배양액 및 감염 무지개송어의 뇌조직 마쇄액을 사용하여 실험하였다. 바이러스 배양액을 이용한 ELISA법의 검출 감도 조사에서 최소 바이러스 감염가 검출 한계치는 10^(2.6) TCID_(50)/100 ㎕ 이었다. 또한, 인공감염어의 뇌조직 마쇄액 내의 RVS 항원도 검출 되었다. 본 방법은 현장에서의 RVS 감염어 조사에 효과적으로 사용되어질 수 있는 방법으로 생각 되어진다. An indirect double antibody enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of a new virus isolated from abnormally swimming salmonid fish, RVS (Retrovirus of salmonid). Results using brain tissue homogenates, and infected cell cultures are described. The sensitivity of the methods is 10^(2.6) TCID_(50)/100 ㎕ of the examined cell culture fluid. The specificity was confirmed by the ELISA inhibition test and virological examinations. Viral antigen could be detected in artificially infected fish tissue homogenates. The assay will allow the diagnosis of RVS-infected fish within a day.
-
해산어 종묘 생산 시기에 발생하는 바이러스성 신경괴사증 ( VNN ) 원인바이러스의 유전학적 비교
김석렬(Suk Ryol Kim),정성주(Sung Ju Jung),김영진(Young Jin Kim),김진도(Jin Do Kim),정태성(Tae Sung Jung),최태진(Tae Jin Choi),오명주(Myung Joo Oh),길수수(Mamoru Yoshimizu) 한국수산과학회 2002 한국수산과학회지 Vol.35 No.3
N/A This study was performed both to explore the host of nervous necrosis virus (NNV) between mariculturing fish species and to examine the phylogenic position of the NNV in Korea. NNV was confirmed on the basis of histopatbological and molecular biological examination, then VNN infection was proved from either moribund or dead fishes including red drum, Sciaenops ocellatus; oblong rock fish, Sebastes oblongus and flounder, Paralichthys olivaceus. As a result of sequencing for a part of NNVs, virus from red drum was showed 98%, 97%, 86% and 74% homology with oblong rock fish, grouper, Japanese flounder and striped jack, respectively. On the other hand, NNV from oblong rock fish was demonstrated 96%, 85% and 72% homology with grouper, Japanese flounder and striped jack, respectively. NNV from red drum and oblong rock fish was exhibited phylogenically distant from the representative NNV, SJNNV originated from striped jack. On the contrary, the viruses appeared to be similar species with Taiwan NNV isolated from culturing grouper.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
