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주화성에 의한 활동성 정자 분리용 마이크로 바이오칩에 관한 연구
고용준(Yongjun Ko),맹준호(Joon-Ho Maeng),안유민(Yoomin Ahn),황승용(Seung Yong Hwang) 대한기계학회 2008 大韓機械學會論文集A Vol.32 No.12
This paper presents a new microchip which can separate motile sperm by chemotaxis. The microchip was developed to create longitudinal concentration gradient in the microchannel due to diffusion. Linearly good concentration gradient of chemoattractant was generated without any fluid control devices. In sperm separation experiment with the developed microchip, mouse sperm was used as sample and acetylcholine was selected as chemoattractant. Human tubal fluid (HTF), buffer solution, was introduced into the microchannel of the microchip and attractants diluted in ratio of 1, 1/2, 1/4, 1/8, 1/16, 1/32 and 1/64 including control (DI water) were dropped in each outlet by 2 ㎕ volume with micropippet. After 5min, 1 ㎕ sperm solution was dropped into inlet of the chip. After 10 min, when sperms reached to the outlet by chemotaxis, we counted sperms in each outlet by using microscopy. Consequently, we could separate progressive motile sperm with the new microchip. In the experiment, the most sperms were isolated at the outlet dropped with 1/16 diluted solution. The optimal concentration gradient to induce chemotaxis was about 0.625 ㎎/㎖/㎜.
조철호(Chul-Ho Cho),고용준(Yong-Joon Ko),안유민(Yoomin Ahn),이성환(Sung-Hwan Lee),조남규(Nahmgyoo Cho),맹준호(Joon-Ho Maeng),이병철(Byung-Chul Lee),황승용(Seung-Yong Hwang) 대한기계학회 2005 대한기계학회 춘추학술대회 Vol.2005 No.5
Immunoassay is a method for clinical diagnoses and biochemical studies with antibody-antigen reaction. However, immunoassay takes a lot of time and expensive reagents. In this research, we report a microbiochip which can detect the electric signal from biochemical reaction with high sensitivity and low sample volume using micro bead. Gold electrode was fabricated on glass substrate using conventional MEMS technology for detecting the electric signal.