Clq-coated ELISA법을 이용한 정맥용 면역글로불린제제의 항보체성 측정

강혜나(Hye Na Kang),김순남(Soon Nam Kim),신광훈(Kwang Hoon Shin),허숙진(Sook Jin Hur) 大韓藥學會 2001 약학회지 Vol.45 No.6

The quality of an intravenous immunoglobulin preparation (IVIG) is reflected by the degree of nonspecific activation of complements, the so-called anticomplementary activity (ACA). ACA of aggregates in IVIG was investigated using method by the European Pharmacopoeia and Clq-coated microtiter enzyme-linked immunosorbent assay (ELISA). Both the EP method and the ELISA method showed a dose response curve with the amount of complements bound increasing with the percentage content of aggiegates in immunoglobulin standard. The correlation between the two tests was good (r=0.96, r=0.99). However the correlation was not found when the ACA (EP method) of IVIG product was compared with its aggregate percentage. These results emphasize that the method of aggregate formation affects ACA and that estimation of the percentage distribution of aggregates by HPLC may not reflect ACA. In analysing IVIG product for Clq binding activity test with the ELISA, the result by using Protein A-HRP correlated with aggregate percentage (r=0.84). But the correlation decreased (r=0.48) when the result used Protein A-AP(having poorer sensitivity than HRP) was compared with aggregate percentage. As a result, some variation between the two methods, due to differences in assay principles, is to be expected. However ELISA technique has the advantage in that it is easier to perform, more precise and less subject to reagent variability and is the more suitable screening method than HPLC analysis.

연인관계 종결 후 스토킹 행위를 하는 일반 성인의 특성에 관한 연구

강혜지(Hye ji Kang),이지혜(Ji Hae Lee),성나경(Na kyung Seong),성현준(Hyun Jun Sung),강지은(Ji eun Kang),홍동규(Dong gyu Hong) 서울사이버대학교 미래사회전략연구소 2024 미래사회 Vol.15 No.1

본 연구는 일반 성인에게서 나타날 수 있는 스토킹 행위의 위험요인을 파악하고자 하였다. 이를 위해 연인관계 종결 후 스토킹 시도 경험, 개인의 성별, 상대와의 첫 만남 특성에 따라 스토킹 위험요인의 차이를 확인하였다. 연구 수행을 위해 헤어진 연인과 재회를 희망하는 대상자(남성 51명, 여성 68명)의 질문지와 접수 상담일지를 수집하여 분석하였다. 주요 연구 결과는 첫째, 남성의 경우 상대방에게 실제적 위협을 가할 수 있는 미행, 자해 및 자살 위협과 같은 스토킹 범죄 특성을 보이는 경향이 있었고, 여성의 경우 가해자 위험요인 중 정신건강 문제가 남성에 비해 높았다. 둘째, 온라인으로 첫 만남을 가진 남성의 경우 고용 및 재정문제와관련된 가해자 위험요인을 더 많이 보였으며, 여성은 오프라인 만남으로 관계를 시작한 경우스토킹 행위의 지속성과 점진적 심각성이 더 높은 것으로 나타났다. 이러한 결과는 범죄자가아니더라도 일반인 간의 교제에서 스토킹 행위가 충분히 일어날 수 있기 때문에 일반인 대상으로 스토킹의 위험성을 강조하는 것이 중요하다는 것을 시시한다. 나아가서 재회상담 장면에서 내담자의 성별, 첫 만남 경위 등을 고려하여 사례를 구조화 시키고, 개입방법을 모색하는것이 중요하다는 것을 의미한다. 또한 본 연구 결과는 재회상담 현장에서 스토킹 위험성 예측및 사전 예방을 위한 대상자 교육의 기틀을 만드는 것이 중요하다는 사실을 시사한다. This study identified risk factors of stalking among general adults. For this reason, we examined whether previous stalking offenses, gender, and initial encounter characteristics cause differences in stalking risk factors. For the study, questionnaires and intake counseling records from individuals (51 males and 68 females who ended their romantic relationships but desired to reunite) were collected and analyzed. The key findings are as follows. First, males exhibited more threatening behaviors such as pursuit, self-harm, and making suicidal threats, while females were highly prevelent of mental health issues. Second, males who initially met their partners online demonstrated higher levels of employment and financial issues. Females who initially met their partners offline, showed higher levels of persistent and severe stalking behavior. The results suggest that it is important to raise awareness among the general public regarding the danger of stalking behavior, as it can frequently occur in relationships involving individuals who are not criminals. Furthermore, it is vital to consider the client’s gender and initial encounter characteristics with their partners for reunion counseling case formulation, and intervention planning. The results also suggest the importance of stalking risk assessment and stalking prevention programs for clients in reunion counseling practice.

합성기질 및 응고시간을 이용한 혈액응고 제8인자 역가측정법

강혜나(Hye Na Kang),김순남(Soon Nam Kim),허숙진(Sook Jin Hur),홍성화(Seung Hwa Hong) 대한약학회 2001 약학회지 Vol.45 No.5

The clotting assay was replaced by the chromogenic substrate assay which is recommended by the European Pharmacopoeia (EP) and the Scientific and Standardization Committee of the International Society on Thrombosis and Haemostasis based on the reliability convenience and simplicity of the chromogenic assay: A correlation study was carried out with a one-stage factor VIII : C clotting assay and the perfonnance of the chromogenic assay was evaluated using two test kits that fulfilled the requirements of EP for factor VIII concentrates test. Although chromogenic assay has partly differences in measurement principle and standardization, this assay has a high correlation with clotting assay in various types of factor VIII concentrates and factor Vlll standard. We conclude that the chromogenic assay for factor VIII : C con- centrates correlates well with the clotting assay and shows good analytical performance.

생쥐 난자 배양시의 자발적 투명대 경화 현상과 PMA에 의한 투명대 경화 현상 비교

강혜나,배인하,김해권,Kang, Hye-Na,Bae, In-Ha,Kim, Hae-Kwon 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1

One consequence of fertilization in mammals is an increased resistance of the zona pellucida (ZP) to proteases and various chemical reagents. This phenomenon has been called 'zona pellucida hardening' (ZPH), and it is generally accepted that it is caused by the secretory products of cortical granules released by the egg at fertilization. ZP of mouse oocytes maturing in vitro in a chemically defined medium becomes progressively more resistant to solubilization by chymotrypsin ("Spontaneous" ZP hardening). In the present study, it was aimed to find the specificity of spontaneous ZPH in relation to its possible relevance to the cortical reaction and the physiological block to polyspermy. When a maturation inhibitors, cAMP analog(dbcAMP) and phosphodiesterase inhibitor (IBMX) was added to culture medium, it prevent spontaneous ZPH of mouse oocyte during in vitro culture. Thus spontaneous ZPH requires GVBD, since it is prevented by those agents, which inhibit GVBD in vitro. However, culture for 3 hours in the presence of PMA(lOng/ml), a protein kinase C activator, resulted in ZPH without GVBD, thus suggesting that ZPH may be regulated independently apart from the event of GVBD. Pretreatment of mouse oocyte with FBS result in partially inhibitory effect on subsequent spontaneous ZPH. Induction of GVBD in vivo had a inhibitory effect on the spontaneous ZPH, but subsequent spontaneous ZPH. Induction of GVBD in vivo had a inhinbitory effect on the spontaneous ZPII, but had no inhibitory effect on PMA-induced ZPH. Treatment with a microfilament formation blocker(cytochalasin-B) at 1${\mu}g$/ml concentration, resulted in the excellent inhibitory effect on spontaneous ZPH. However cytochalasin-B did not inhibit PMA-induced ZPH. Thus this suggesting that spontaneuse ZPH had a different mechanism from PMA-induced ZPH.

생쥐 난자 배양시 외분비 관련 요소들이 자발적 투명대 경화 현상에 미치는 영향

강혜나,배인하,김해권,Kang, Hye-Na,Bae, In-Ha,Kim, Hae-Kwon 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1

"Spontaneous" hardening of the zona pellucida of mouse oocytes during in vitro culture is most likely due to cortical granules exocytosis. Thus the purpose of the present study was to determine whether the exocytosis factor is involved in spontaneous zona pellucida hardening during in vitro culture of the mouse. The results obtained form these experiments were summarized as follows; 1. When a protein synthesis inhibitor(100${\mu}g$/ml puromycin) was added to the culture medium, it did not prevent spontaneous ZPH of mouse oocyte during in vitro culture. 2. Calmodulin antagonists (trifluoperazine and chlorpromazine) and calcium channel blocker (verapamil) had no inhibitory effect in spontaneous ZPH. 3. A microtubule assembly inhibitor, colcemid had some inhibitory effect on spontaneous ZPH. 4. Treatment with a microfillament formation blocker(cytochalasin-B) at 1${\mu}g$/ml concentration, resulted in the excellent inhibitory effect on spontaneous ZPH. However cytochalasin-B did not inhibit ethanol-induced ZPH.

PCR법을 이용한 혈액 및 혈액제제 중 HBV검출

임재현(Jae-Hyun Lim),강혜나(Hye-Na Kang),최승은(Seung-Eun Choi),정사라(Sa-Raah Jung),박순희(Sue-Nie Park),민홍기(Hong-Ki Min),홍성화(Seung-Hwa Hong) 대한미생물학회 2005 Journal of Bacteriology and Virology Vol.35 No.2

유전자 증폭기술에 의한 혈액 내 인체파보바이러스 B19의 검출

김홍란(Hong Ran Kim),조성관(Sung Kwan Jo),이규철(Gyu Cheol Lee),이현아(Hyun A Yi),강혜나(Hye Na Kang),홍성화(Sung Hwa Hong),이찬희(Chan Hee Lee) 대한미생물학회 2002 Journal of Bacteriology and Virology Vol.32 No.3

Vero Cell과의 공동배양이 체외에서 생쥐 난자성숙과 배아발생에 미치는 영향

강혜나,하종식 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

Assisted reproductive technology(ART)have contributed significantly to alleviating subfertility in the childless couple. In spite of the many advances in the field of ART, the pregnacy and take-home baby rates for in virto fertilization(IVF)have been very poor. In order to overcome these problems, a variety of coculture systems has been devised. Vero cells, derived from African green monkey kidney, were selected because kidney and genital tract have a common embryonic origin. In addition, these cells are safe for coculture with embryos:they are highly controlled for viruses and other contaminants because they are used for vaccine production. Several investigators showed that coculturing human embryos with Vero cells in vitro resulted in an improvement of embryo development. However, they did not observe the same results using mouse oocytes and embryos. We thus designed a series of experiments to demonstrate whether or not Vero cells do indeed enhance mouse oocyte maturation and embryo development. In this experiment, Vero cell does not allow the mouse immature oocytes to be enhanced maturation rate in vitro. To study the 'In-Vitro 2-Cell Block' in mouse embryo, we have cocultured ICR one-cell mouse embryos with Vero cell in different medium. In Ham's F-10 the mouse embryos arrested their development prior to 4-cell stage(control 76.7%:coculture 75.0%). In contrast, the coculturing mouse embryos revealed enhanced development(control 0%;coculture 22.8%) in human tubal fluid(HTF)only in late embryonic stages(hatching). On the other hand, the degree of blastomere fragmentation exhibited a reverse trend to that of the developmental capacity. Embryos from coculture groups(Ham's F-10 & HTF)showed some fragmentation(0% & 4.2%)while 13.3% and 14.3% of the embryos in control groups(Ham's F-10 & HTF)were severely fragmented (P<0.05). Thus the use of coculture systems appears to be dependent on the type of medium used as a support. The development rate of late 2-cell mouse embryos in Vero cell coculture was no significant differences until blastocyst stage but improved at late develpmental stage(control 42.1% : coculture 70.7%). Thus the Vero cell coculture system was shown to increase the hatching rate of mouse embryos.

Vero Cell-Conditioned Medium에서의 배양이 생쥐 후기 2-세포기 배아 발생에 미치는 영향

강혜나,하종식 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.4

본 실험결과 Vero cell과의 공동배양과 Vero cell-conditioned medium에서의 배양은 생쥐 후기 2-세포기 배아의 부화율을 놓이고 세편이 적은 양질의 배아획득을 돕고 세포 증식을 빠르게 해 팽윤 배반포시 할구수를 증가시키는 것으로 나타났다. 그러나 앞으로 공동배양 기작에 작용할 것으로 보이는 embryotrophic factor나 배양액에서 공동배양 세포주에의해 제거되는 발생저해 물질 등의 분자생물학적 연구가 진행되어야하고, 수정시 정자등이 공동배양 세포주의 monolayer에 결합하여 수정 기회를 감소시켜 여러 장점에도 불구하고 오히려 수정률을 감소시킬 수 있는 적접적인 공동배양보다 conditioned medium에서의 배양에 관심을 가져야할 것으로 본다. This study was conducted to evaluate the ability of Vero cell-conditioned medium for supporting mouse embryo development in vitro. The mouse late 2-cell embryos were cultured in control media(Ham's F-10 +10%FBS), media with Vero cell monolayer and Vero cell-conditioned media for 4 days and measured the hatching rate and cell number in the blastocyst stage. The hatching rate in experimental groups was increased significantly compared with embryos in control group(p<0.01). On the other hand, the degree of blastomere fragmentaion exhibited a opposite trend to that of the developmental capacity(p<0.05). And also the cell numbers of expanded blastocysts in experimental groups were increased significantly compared with the control group(p<0.001). There was, however, no difference between experimental groups. These results indicate that Vero cell-conditioned medium supported the mouse embryo development as a Vero cell monolayer. And the mechanism for enhancement of the development potential of embryos may be releasing the embryotrophic factor during the medium-conditioning period.

Vero Cell-Conditioned Medium에서의 배양이 생쥐 후기 2-세포기 배아 발생에 미치는 영향

강혜나,하종식 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

본 실험결과 Vero cell과의 공동배양과 Vero cell-conditioned medium에서의 배양은 생쥐 후기 2-세포기 배아의 부화율을 높이고 세편이 적은 양질의 배아획득을 돕고 세포 증식을 빠르게 해 팽윤 배반포시 할구수를 증가시키는 것으로 나타났다. 그러나 앞으로 공동배양 기작에 작용할 것으로 보이는 embryotrophic factor나 배양액에서 공동배양 세포주에 의해 제거되는 발생저해 물질 등의 분자생물학적 연구가 진행되어야 하고, 수정시 정자등이 공동배양 세포주의 monolayer에 결합하여 수정 기회를 감소시켜 여러 장점에도 불구하고 오히려 수정률을 감소시킬 수 있는 직접적인 공동배양보다 conditioned medium에서의 배양에 관심을 가져야할 것으로 본다. This study was conducted to evaluate the ability of Vero cell-conditioned medium for supporting mouse embryo development in vitro. The mouse late 2-cell embryos were cultured in control media(Ham's F-10+10%FBS), media with Vero cell monolayer and Vero cell-conditioned media for 4 days and measured the hatching rate and cell number in the blastocyst stage. The hatching rate in experimental groups was increased significantly compared with embryos in control group(p<0.01). On the other hand, the degree of blastomere fragmentation exhibited a opposite trend to that of the developmental capacity(P<0.05). And also the cell numbers of expanded blastocysts in experimental groups were increased significantly compared with the control group(p<0.001). There was, however, no difference between experimental groups. These results indicate that Vero cell-conditioned medium supported the mouse embryo development as a Vero cell monolayer. And the mechanism for enhancement of the development potential of embryos may be releasing the embryotrophic factor during the medium-conditioning period.