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한국인 주의력결핍 과잉행동장애와 세로토닌 1B 수용체 유전자 다형성의 관련성:가족기반 연구 및 환자-대조군 연구
박태원,김붕년,임명호,유희정,강대희,정영철,Park, Tae Won,Kim, Boong Nyun,Im, Myung-Ho,Yoo, Hee Jeong,Kang, Daehee,Chung, Young-Chul 대한생물정신의학회 2004 생물정신의학 Vol.11 No.2
Objective:Attention deficit hyperactivity disorder(ADHD) is the most common childhood psychiatric disorder, affecting 3-5% of school-aged children. Although the biological basis of ADHD is unknown, family studies provide strong evidence that ADHD has a genetic basis. Recent genetic studies have suggested associations between ADHD and serotonin 1B(5HT1B) receptor gene G861C polymorphism. The aim of this study is to test for the association between ADHD and 5HT1B receptor gene G861C polymorphism in Korean population. Method:We processed DNA extraction and genotyping. 106 Korean children with ADHD and their parents were analyzed using the transmission disequilibrium test(TDT) and haplotype-based haplotype relative risk (HHRR). And the ADHD children were compared with 212 age and gender matched normal controls. Results:There was no statistical difference of distributions between ADHD cases and controls. We did not observe any preferential transmission of alleles of 5HT1B receptor gene G861C polymorphism in ADHD. Conclusions:Though there is the possibility of failing to detect small genetic effects, our results show no evidence of an association between ADHD and 5HT1B receptor gene G861C polymorphism in the Korean population and indicate that it is unlikely that the 5HT1B receptor is implicated in the susceptibility to ADHD.
산업폐기물 소각장 근로자에서 요중 PAHs 대사 산물과 혈중 aromatic-DNA adducts
이경호(Kyoung-Ho Lee),Masayoshi Ichiba(Masayoshi Ichiba),최인미(Inmi Choi),최재욱(Jae-wook Choi),조수헌(Soo-Hun Cho),강대희(Daehee Kang) 한국환경성돌연변이발암원학회 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.4
This study evaluated the concentrations of urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) in industrial waste incineration workers. The effect of genetic polymorphisms of xenobiotic metabolism enzymes on urinary concentration of PAH metabolites was assessed. And, aromatic DNA adduct levels were<br/> also determined in total white blood cells. Fifty employees were recruited from a company handling industrial wastes located in Ansan, Korea: non-exposed group (n=21), exposed group (n=29). Sixteen ambient PAHs were determined by GC/MSD (NIOSH method) from personal breathing zone samples of nine subjects near incinerators.<br/> Urinary 1-hydroxypyrene glucuronide (1-OHPG), a major pyrene metabolite, was assayed by synchronous fluorescence spectroscopy after immunoaffinity purification using monoclonal antibody 8E11 (SFS/IAC). Multiplex PCR was used for genotyping for GSTM1/T1 and PCR-RFLP for genotyping of CYP1A1 (MspI and Ile/Val). PAH-DNA adducts in peripheral blood WBC were measured by the nuclease P1-enhanced postlabeling assay. Smoking habit, demographic and occupational information were collected by self-administered questionnaire. The range of total ambient PAH levels were 0.00-7.00 mg/㎥ (mean 3.31). Urinary 1-OHPG levels were significantly higher in workers handling industrial wastes than in those with presumed lower exposure to PAHs (p=0.006, by Kruskal-Wallis test). There was a statistically significant dose-response increase in 1-OHPG levels<br/> with the number of cigarettes consumed per day (Pearson correlation coefficient=0.686, p<0.001). Urinary 1-OHPG levels in occupationally exposed smoking workers were highest compared with non-occupationally exposed smokers (p=0.053, by Kruskal-Wallis test). Smoking and GSTM1 genotype were significant predictors for<br/> log-transformed 1-OHPG by multiple regression analysis (overall model R²=0.565, p<0.001), whereas smoking was the only significant predictor for log-transformed aromatic DNA adducts (overall model R²=0.249, p=0.201). Aromatic DNA adducts was also a significantly correlation between log transferred urinary 1-OHPG levels (pearson's correlation coefficient=0.307, p=0.04). However, the partial correlation coefficient adjusting for Age, Sex, and<br/> cigarette consumption was not significant (r=0.154, p=0.169). The significant association exists only in individuals with the GSTM1 null genotype (pearsons correlation coefficient=0.516, p=0.010; partial correlation coefficient adjusting for age, sex, and cigarette consumption, r=0.363, p=0.038). Our results suggest that the significant increase in urinary 1-OHPG in the exposed workers is due to higher prevalence of smokers among them, and that the association between urinary PAH metabolites and aromatic DNA adducts in workers of industrial waste handling may be modulated by GSTM1 genotype. There results remain to be confirmed in future larger studies.
소각장 근로자에서 GSTM1의 유전자 다형성이 glycophorin A 변이 발현율과 소변내 PAH 대사산물 농도와의 관계에 미치는 영향
이경호(Kyoung-Ho Lee),하미나(Mina Ha),최재욱(Jae-Wook Choi),조수헌(Soo-Hun Cho),박정규(Chung-Gyu Park),황응수(Eung-Soo Hwang),강대희(Daehee Kang) 한국환경성돌연변이발암원학회 2001 한국환경성돌연변이·발암원학회지 Vol.21 No.2
Eighty-one workers including 38 employees directly incinerating industry wastes were recruited from a company located in South Korea. To evaluate the association between urinary 1-hydroxypyrene glucuronide (1-OHPG) levels, as internal dose of polycyclic aromatic hydrocarbon (PAH) exposure, and glycophorin A (GPA) mutation frequency, as an early biologic effect indicator. Urinary 1-OHPG levels were measured by synchronous fluorescence spectroscopy after immunoaffinity purification using monoclonal antibody 8E11. Erythrocyte GPA variant frequency (NN or NO) was assessed in MN heterozygotes with a flow cytometic assay. The GSTM1 and GSTT1 genotypes were assessed by a multiplex PCR method. The GPA NN phenotype frequency was higher in occupationally exposed group (n=14, mean±S.D. 6.6±12.0 in 106 erythrocyte cells)<br/> than in non-exposed group (n=22, 2.1±3.5). Similarly, the GPA(NO or NN) phenotype frequency was higher in exposed group (n=14, 9.7±17.3) than non-exposed group (n=22, 4.2 ± 6.3). The above differences failed to reach statistical significance, but a significant increase was seen in GPA variant frequency levels with increase in urinary 1-OHPG levels (Spearman's correlation: p=0.06 (NO), p=0.07 (NO or NN)). When this association was evaluated by GSTM1 genotype status, the association between GPA mutation and urinary 1-OHPG levels was stronger in individuals with GSTM1 present genotype (Spearmans correlation; r=0.50, p=0.02). These<br/> results suggest that the association between urinary 1-OHPG and GPA mutation is be modulated by the GSTM1 genotype.
콜타르가 함유된 페이트 사용 조선업 근로자에서 요중 1-hydroxypyrene glucuronide와 대사효소 유전자 다형성에 관한 연구
이경호(Kyoung-Ho Lee),이정미(Jeongmi Lee),최인미(Inmi Choi),김재용(Jaiyong Kim),임형준(Hyungjune Im),이상윤(Sang-Yun Lee),윤기정(Kijung Yoon),고상백(Sang Back Koh),최홍렬(Hong Ryul Choi),조수헌(Soo-Hun Cho),강대희(Daehee Kang) 한국환경성돌연변이발암원학회 2000 한국환경성돌연변이·발암원학회지 Vol.20 No.1
Although shipbuilding workers were exposed to a variety of genotoxic compounds including polycyclic aromatic hydrocarbons (PAHs), limited number of studies were conducted to evaluate the biomarkers related to PAH exposure in painting workers in shipbuilding industry. One hundred and thirty three workers<br/> including 73 employees using coal tar paints were recruited from a shipbuilding company located in South Korea. Urinary 1-hydroxypyrene glucuronide (1-OHPG), as internal dose of PAH exposure, were measured by synchronous fluorescence spectroscopy after immunoaffinity purification using monoclonal antibody 8E11. Glutathione S-transferase (GST)M1 and GSTT1 genotypes were assessed by multiplex PCR. Information on demographic characteristics, smoking habit, diet, job title, use of personal protective equipments were collected by self-administered questionnaire. Urinary 1-OHPG were higher in workers using coal tar paints than in workers using general paints, however, the difference was not statistically significant (p=0.20, Mann-Whitney U test). Urinary<br/> 1-OHPG levels in smokers were higher than in non-smokers (p<0.05 by Mann-Whitney U test) and there was a significant increase in urinary 1-OHPG levels with the numbers of cigarettes consumed per day (Spearman's correlation coefficient=0.28, p=0.02). Genetic polymorphisms of GSTM1 and GSTT1 did not influence the level of 1-OHPG in study subjects. Multiple regression analysis show that smoking is the only significant predictor for log-transformed 1-OHPG (overall model R²=0.1). These results suggest that workers using coal tar paints were exposed to significant amount of PAHs and individual difference in xenobiotic metabolism might affect the<br/> levels of internal dose of PAHs.
사무실 근로자들에서의 sick building syndrome
강대희 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.3
A cross-sectional study was conducted to evaluate the relationship between sick building syndrome (SBS) and the factors affecting SBS among 90 office workers. The study consisted of 1) a review of previous environmental investigations, 2) measurement of CO₂, temperature, and relative humidity (RH), 3) a questionnaire survey of symptom prevalence and perception of environmental conditions using the National Institute for Occupational Safety and Health Indoor Air Quality (NIOSH IAQ) questionnaire, and 4) confidential interview and a review of medical records of employees having respiratory symptoms. CO₂, temperature, and RH measured on the day of questionnaire survey were within the recommended range by American Society of Heating, Refrigerating, and Air-Conditioning Engineers (ASHRAE). Sixty-six percent of respondents were women and 60% considered their job description either "managerial(28%)" or "professional(32%)". Respondents had worked in the building for an average of 5.8 years. Forty-four percent reported having SBS defined as "having one or more symptoms that had occurred at work one or more days a week and tended to get better when away from work". Sex(p=0.001), duration of computer use(p=0.02), use of laser printer(p=0.02), use of cleanser or other office chemicals(p=0.004), feeling too little air movement(0.001), feeling air too dry(p=0.001), and unsatisfied with the current job(p=0.02) were related to an increased prevalence of SBS. Use of cleanser or other office chemicals(p=0.01), feeling too little air movement(p=0.01), and feeling air too dry(p=0.02) remained significant predictors of SBS when adjusting other variables by logistic regression analysis. The results of medical record review revealed a discrepancy in the number of diagnosed asthma cases by personal physician an dan independent physician. Although this study contains several limitations (e.g. cross-sectional study, small numbers of study subjects, etc.), these results indicated that SBS is related to personal, environmental, and psychosocial factors and the precision of diagnosis is critical when evaluating environment-relatedness during disease cluster investigation.