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Molecular characterization of chemosensory protein genes in Bactrocera minax (Diptera: Tephritidae)
Wang Zhao‐Xiang,Qi Zhen‐hua,Chen Jian,Wang Fu‐Lian,Gui Lian‐You,Zhang Guo‐Hui 한국곤충학회 2021 Entomological Research Vol.51 No.7
Chemoreception is of great importance for survival of insects. Chemosensory proteins (CSPs) are believed to be involved in the perireceptor events of chemosensory system in many insect species, but this has not been clarified in the citrus fruit fly, Bactrocera minax, a serious dipteran pest of citrus crops. Previous studies indicated that four CSP genes were identified in many fly speices in Diptera. In the present study, we also identified four CSP genes in B. minax,namely BminCSP1–4, from a transcriptome database. All CSP proteins encoded by these genes bear the typical hallmarks of the CSP family: an N-terminal signal peptide and the four highly conserved cysteine residues. Phylogenetic analysis comparing with other dipteran CSPs indicated that dipteran CSPs may evolved from three ancestral CSP genes, and revealed the sequence diversities of BminCSPs and showed that BminCSP1–4 are clustered in separate groups, indicating the possibility of their contrasting function in B. minax. Furthermore, the tissue distribution of the four CSP genes in adult B. minax was analyzed by real-time quantitiative PCR. The results demonstrated that BminCSP3 are significantly transcriptionally enriched in antennae; BminCSP4 expressed primarily in heads; BminCSP1 and BminCSP2 showed high expressions in different tissues, such as antennae, abdomens and wings. Based on these findings, the different implications for the functions of BminCSPs are discussed. This study will offer a significant indication for further functional studies of the CSPs in B. minax
Auspicious Animal Patterns on Traditional Chinese Furniture Design
( Wang Xiang ) 동양예학회 2018 東洋 禮學 Vol.40 No.-
This paper explores the auspicious animal patterns of traditional Chinese furniture. The meaning of the auspicious patterns is full of the motifs, which are originated from the soul of Chinese traditional culture. The patterns show the great achievements in furniture decoration, which embody people’s desires for a better life. All kinds of patterns are expressed the best wishes. The patterns of Chinese traditional furniture include plants, animals, religious motifs, auspicious characters and etc,. In these subjects, the animal patterns are not only expressed the auspicious meaning alone, but also embodied and mixed with other themes, which enrich the form of patterns.
Investigation on MRR and Machining Gap of Micro Reciprocated Wire-EDM for SKD11
Xiang Chen,Zhen-long Wang,Yukui Wang,Guanxin Chi 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.1
In this paper, the investigational work is carried out on the micro reciprocated wire electrical discharge machining (wire-EDM) of SKD11 using circularly travelled wire with Φ30 μm as tool electrode, aiming to achieve the fabrication of micro parts with high cost-effect, efficiency and precision. The experiments are systematically performed using central composite design approach of response surface methodology to study the effect of open voltage, discharge capacitance, pulse duration and feed rate on material removal rate (MRR) and machining gap. The mathematical models of MRR and machining gap are developed by analysis of variance, and whilst verified by confirmation experiments with ≤ 8.38% and 6.70% prediction errors for MRR and machining gap, respectively. Besides, multi-objective optimization of parameter combinations is attempted by non-dominated sorting genetic algorithm-II. Finally, a typical experimental sample, micro gear is successfully machined with 1194.38 μm tip diameter under the condition of No. 14 optimal solution.
Wang, Fu-yu,Wang, Peng,Yang, Chen-xuan,Zhou, Tao,Jiang, Jin-li,Meng, Xiang-hui The Korean Neurosurgical Society 2020 Journal of Korean neurosurgical society Vol.63 No.4
Objective : An important factor during pituitary adenoma surgery is to preserve pituitary stalk (PS) as this plays a role in reduction of the risk of postoperative diabetes insipidus. The hypothalamic-hypophyseal tract (HHT) projects through the PS to the posterior pituitary gland. To reconstruct white matter fiber pathways, methods like diffusion tensor imaging (DTI) tractography have been widely used. In this report we attempted to predict the position of PS using DTI tractography and to assess its intraoperative correlation during surgery of pituitary adenomas. Methods : DTI tractography was used to tract the HHT in nine patients before craniotomy for pituitary adenomas. The DTI location of the HHT was compared with the PS position identified at the time of surgery. DTI fiber tracking was carried out in nine patients prior to the planned craniotomy for pituitary adenomas. In one patient, the PS could not be identified during the surgery. In the other eight patients, a comparison was made between the location of the HHT identified by DTI and the position of the PS visualized at the time of surgery. Results : The position of the HHT identified by DTI showed consistency with the intraoperative position of the PS in seven patients (88.9% concordance). Conclusion : This study shows that DTI can identify the position of the HHT and thus the position of the PS with a high degree of reliability.
Wang, Ya-Dong,Zhai, Wen-Long,Wang, Hai-Yu,Xia, Xiang-Qun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11
Background: A number of studies have reported the association of X-ray repair cross-complementing group 1 (XRCC1) Arg399Gln polymorphism with susceptibility to hepatocellular carcinoma (HCC). However, the results were inconsistent and inconclusive. The aim of this study was to comprehensively explore the association of XRCC1 Arg399Gln variant with HCC risk. Materials and Methods: Systematic searches of PubMed, Elsevier, Science Direct, CNKI and Chinese Biomedical Literature Database were performed. Pooled odds ratio (OR) with 95% confidence intervals (CI) was calculated to estimate the strength of association. Results: Overall, we observed an increased HCC risk among subjects carrying XRCC1 codon 399 Gln/Gln, Arg/Gln and Gln/Gln+Arg/Gln genotypes (OR=1.20, 95%CI: 1.05-1.38, OR=1.16, 95%CI: 1.05-1.28, and OR=1.14, 95%CI: 1.04-1.24, respectively) based on 20 studies including 3374 cases and 4633 controls. In subgroup analysis, we observed an increased risk of XRCC1 codon 399 Gln/Gln, Arg/Gln and Gln/Gln+Arg/Gln polymorphisms for HCC in hospital-based study (OR=1.25, 95%CI: 1.03-1.51, OR=1.21, 95%CI: 1.07-1.36 and OR=1.18, 95%CI: 1.06-1.31, respectively) and in Asian population (OR=1.19, 95%CI: 1.03-1.38, OR=1.17, 95%CI: 1.04-1.30 and OR=1.14, 95%CI: 1.04-1.25, respectively). Limiting the analysis to the studies with controls in agreement with Hardy-Weinberg equilibrium (HWE), we observed an increased HCC risk among Gln/Gln, Arg/Gln and Gln/ Gln+Arg/Gln genotype carriers (OR=1.17, 95%CI: 1.05-1.29, OR=1.12, 95%CI: 1.00-1.25 and OR=1.11, 95%CI: 1.02-1.21, respectively). Conclusions: This updated meta-analysis results suggest that XRCC1 Arg399Gln variants may contribute to HCC risk. Well-designed studies with larger sample size were required to further verify our findings.
Effect of P3HT passivation layer on triple cation organic-inorganic hybrid perovskite memristor
Wang Yucheng,Xiong Yuxuan,Wang Hongsu,Wu Xiang,Sha Jian,Shang Yueyang,Zhang Yongqi,Li Wei,Wang Shaoxi 한국물리학회 2023 Current Applied Physics Vol.47 No.-
Triple cation organic-inorganic hybrid perovskite (TCP) memristor is a novel candidate for artificial visual applications due to the integrating of recognition, storage, and computing. However, concerns remain regarding the moisture-induced degradation and thermal stability. Here, P3HT was deposited on the TCP film to optimize the memristor performance which showed better film surface quality and lower power consumption. When the thickness of the P3HT layer increases, the grain boundary of the TCP film was gradually filled, leading to the inhibition of the ions/vacancies drifting, and thus the device type was changed from digital to analog. Besides, various functions are demonstrated in TCP photoelectric memristor including excitatory postsynaptic current under optic and electric stimulations and spike rate-dependent plasticity, proving the great application prospects in numerical calculation and information perception.
( Xiang Xiao ),( Feng Ping Wang ),( Xu Ke ),( Xi Xiang Tang ),( Ying Bao Gai ),( Muhammad Aamer Mehmood ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.3
A psychrotrophic bacterium, Arthrobacter sp. ON14, isolated from Antarctica, was shown to exhibit a high β-galactosidase activity at a low temperature. A genomic library of ON14 was constructed and screened for β-galactosidase genes on functional plates containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) as the substrate. Two different β-galactosidase genes, named as galA, galB, were found in ON14. Computational analyses of the genes revealed that the encoded protein GalA belongs to family 2 of glycosyl hydrolysases and is a cold-active protein, whereas GalB belongs to family 42 of glycosyl hydrolysases and is a mesophilic protein. Reverse transcription analyses revealed that the expression of galA is highly induced at a low temperature (4℃) and repressed at a high temperature (28℃) when lactose is used as the sole carbon source. Conversely, the expression of galB is inhibited at a low temperature and induced at a high temperature. The purified GalA showed its peak activity at 15℃ and pH 8. The mineral ions Na+, K+, Mg2+, and Mn2+ were identified as enzyme activators, whereas Ca2+ had no influence on the enzyme activity. An enzyme stability assay revealed that the activity of GalA is significantly decreased when it is incubated at 45℃ for 2 h, and all its activity is lost when it is incubated at 50℃.
Application of Laparospic Ultrasonography in Surgery of Small Renal Cell Carcinoma
Wang, Xiang-Zhu,Yu, Ze-Xing,Guo, Rui-Jun,Xing, Nian-Zeng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21
Purpose: : To assess the clinic value of application of laparospic ultrasonography (LU) in partial nephrectomy of small renal cell carcinoma. Materials and Methods: From 2007 to 2011, 28 small renal cell carcinoma patients in ou clinic underwent laparoscopic partial nephrectomy with LU. For comparison with preoperative conventional ultrasound and CT, we collected ultrasonic performance of the affected side kidney, renal tumor location, size, echo change, blood supply situation and the relationship with the surrounding tissue. Results: LU could more clearly show the tumor interior structure and blood supply, as well as the relationship with the surrounding tissue. It also can provided doctor assistance with real-time tumor resection, reducing operative complications. Conclusions: LU can clearly show tumor internal structure and blood supply, which is helpful for explicit diagnosis. Moreover, it supplies accurate information for surgeons and assists surgery. Therefore LU has an important guiding value in partial nephrectomy for small renal cell carcinoma.