10th Korea-Japan Joint International Session : Poster Presentation ; Degradation of Dioxins and Growth of White-Rot Fungus in Contaminated Soil

( Shinya Suzuki ),( Takashi Miyahara ),( Shinya Nakatsuru ),( Yasushi Matsufuji ),( Ichiro Kamei ),( Ryuichiro Kondo ) 한국폐기물자원순환학회(구 한국폐기물학회) 2006 한국폐기물자원순환학회 심포지움 Vol.2006 No.1

매립지에서 PCDDs/PCDFs 의 생분해 가능성과 거동

( Shinya Suzuki ),( Ryuji Yanase ),( Yasushi Matsufuji ),( Ryuichiro Kondo ) 한국폐기물자원순환학회 2002 APLAS Vol.2002 No.2

매립 실험은 대규모의 라이시미터를 이용하여 침출수의 수질 분석을 통하여 침출 가능성과 생분해 가능성을 해명하기 위해 수행되었다. 이 라이시미터는 “white-rot fungi”와 고농도의 PCDDs/PCDFs 를 포함한 비산재, 바닥재, 그리고 불연성의 분쇄 잔재물이 포함된 복토층을 가진다. 본 연구에서 우리는 유전학적 분석과 모니터링을 통하여 세포 조직체의 검출로부터 매립된 폐기물 층과 오염된 토양증에서 white-rot flungi(Ceriporiasp. MZ-340)의 기본 특성을 조사했다. 3 년여에 걸친 대규모의 매립지 실험의 침출수 수질 분석의 연구로부터 도출된 실험용 라이시미터에 매립된 폐기물 층으로부터 침출수로 이동한 PCDDs/PCDFs 의 양은 1.15”~10-4%.에 불과했다. 이것은 대부분의 PCDDs/PCDFs 가 폐기물층에 남아 있다는 것을 의미한다. 침출수의 용출의 경우, PCDDs/PCDFs 는 부유 고형물(SS)과 같은 불용성 물질에서 검출되는 경향이 있다. 게다가, 관찰 3 년째에는, 대부분의 PCDDs/PCDFs 가 침출수에서 검출되지 않았다. 따라서 대부분의 PCDDs/PCDFs 는 매립지에 남아 있다고 결론내릴 수 있다. 그러므로 매립 초기에 부유 고형물 제거를 위한 대안에 초점을 맞춤으로써 PCDDs/PCDFs 를 침출로부터 막는 것이 중요하다. White-rot fungi 의 검출을 위한 유전학적 분석은 폐기물 매립층의 조건에서 살아남을 수 있는 생물체는 없다는 것은 보이고 있다. 기본적인 분해실험의 결과는 white-rot fungi 는 적당한 조건만 주어진다면 높은 성장 잠재력을 가지고, 비산재와 결합된 경우, 개체수가 단기간에 최대에 이를 수도 있음을 보인다. 생물정화를 위한 조건을 잡는 것이 필요하다. A landfill experiment was conducted to clarify the leachability and possibility of biodegradation in a landfill site through leachate quality analysis using a large-scale lysimeter. This lysimeter had a cover soil layer consisting of “white-rot fungi’’ and a waste layer consisting of fly ash including high concentrations of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDDs/PCDFs), bottom ash, and the crushed residue of incombustibles. In this study we also investigated the fundamental properties of white-rot fungi (Ceriporia sp. MZ-340) in landfilled waste layers and the contaminated soil from the detection of cell bodies through genetic analysis and monitoring. From a study of leachate quality analysis of a large-scale landfill experiment carried out over 3 years, the amount of PCDDs/PCDFs moving into the leachate from the landfilled waste layer in the experimental lysimeter was only 1.15×10^-4%. This means that most of the PCDDs/PCDFs remain in the waste layer. In the case of the elution of the leachate, PCDDs/PCDFs tend to be detected in insoluble materials, such as suspended solids. Further more, in the third year of observation, almost none of the PCDDs/PCDFs were detected in the leachate. It can therefore be concluded that most of the PCDDs/PCDFs remained in landfill sites. It is therefore important to prevent PCDDs/PCDFs from leaching by focusing on counter measures for the removal of suspended solids in the initial stage of landfilling. Genetic analysis for the detection of white-rot fungi also showed that there is no organism capable of surviving the conditions of the waste landfill layer. As a result of a fundamental degradation experiment, that showed that the growth potential of white-rot fungi is high if suitable conditions have been met, and that the number of fungi reaches its maximum in a short period of time, even when combined with fly ash. It is essential to consider the setting conditions for bioremediation.

UV Effect on Plant Growth

Kondo, Noriaki,Tou, Seiji,Takahashi, Shinya,Nakajima, Nobuyoshi Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

UV-B radiation gives harmful effects on plants, such as production of several types of DNA lesions, and growth inhibition. On the other hand, plants have some protective mechanisms, including filtering effect due to accumulation of phenolic compounds in epidermal cells and reactivation of DNA lesions, which are enhanced by UV-B irradiation. We have investigated the mechanism of UV-B effects on plants using cucumber seedlings as plant materials. Cucumber plants were cultivated in an artificially lit growth chamber. Supplemental UV-B irradiation, of which intensity was almost equal to the level of natural sunlight, retarded the growth of first leaves. The growth retardation must result trom the inhibition of cell division and/or cell growth. Microscopical observation of leaf epidermis suggested that the growth retardation might be mainly caused by cell growth inhibition. The retardation was, however, restored within 2 or 3 days after the termination of UV-B irradiation. It is known that UV-B irradiation lowers the activity of photo system II (PS II). In the present experimental conditions, however, UV-B irradiation has little effect on PS II activity as estimated by chlorophyll fluorescence. The stomatal conductance, a major factor determining photosynthetic rate, of first leaves increased during the growth. The increase of stomatal conductance was suppressed by UV-B irradiation and restored by termination of the irradiation. It has not been clear, however, what mechanisms are involved in the suppression of increase of stomatal conductance.

Atomization Characteristics of Internal Mixing Twin Fluid Atomizer

(Kim Sang Jin),(Shinya Kondo),(Keiya Nishida),(Hiro Hiroyasu) 한국액체미립화학회 1995 한국액체미립화학회 학술강연회 논문집 Vol.- No.-

Production of honokiol and magnolol byMagnolia obovata callus cultures

( In Sun Park ),( Susumu Kondo ),( Keisuke Oyama ),( Shinya Kajita ),( Ryo Funada ),( Takafumi Kubo ) 韓國木材工學會 2007 한국목재공학회 학술발표논문집 Vol.2007 No.-

Direct Observation of DW Motion Induced Nano-second Current Pulse

Hironobu Tanigawa,Kuniaki Yano,Kouta Kondo,Shinya Kasai,Teruo Ono 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Preoperative lymphocyte-to-C-reactive protein ratio predicts hepatocellular carcinoma recurrence after surgery

Masashi Utsumi,Masaru Inagaki,Koji Kitada,Naoyuki Tokunaga,Midori Kondo,Yuya Sakurai,Kosuke Yunoki,Ryosuke Hamano,Hideaki Miyasou,Yousuke Tsunemitsu,Shinya Otsuka 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.103 No.2

Purpose: This study was performed to determine the prognostic value of lymphocyte-to-CRP ratio after curative resection for hepatocellular carcinoma. Methods: Between July 2010 and October 2021, 173 consecutive patients (144 male, 29 female) who underwent surgical resection for pathologically confirmed hepatocellular carcinoma were included in this retrospective study. Cox regression analysis was used to evaluate the relationship between clinicopathological characteristics and recurrence-free survival (RFS) and overall survival (OS). A P-value of <0.05 was considered statistically significant. Results: The patients (mean age, 71 years) were stratified into high (≥9,500, n = 108) and low (<9,500, n = 65) lymphocyte- to-CRP ratio groups. The low lymphocyte-to-CRP ratio group had significantly worse RFS and OS. Low lymphocyte-to- CRP ratio (hazard ratio [HR], 1.865; 95% confidence interval [CI], 1.176–2.960; P = 0.008), multiple tumors (HR, 3.333; 95% CI, 2.042–5.343; P < 0.001), and microvascular invasion (HR, 1.934; 95% CI, 1.178–3.184; P = 0.009) were independently associated with RFS, whereas low albumin-to-globulin ratio (HR, 2.270; 95% CI, 1.074–4.868; P = 0.032), α-FP of ≥25 ng/mL (HR, 2.187; 95% CI, 1.115–4.259; P = 0.023), and poor tumor differentiation (HR, 2.781; 95% CI, 1.041–6.692; P = 0.042) were independently associated with OS. Lymphocyte-to-CRP ratio had a higher area under the curve (0.635) than other inflammation-based markers (0.51–0.63). Conclusion: Lymphocyte-to-CRP ratio is superior to other inflammation-based markers as a predictor of RFS in patients with surgically resected hepatocellular carcinoma.

Review : EUS-Guided Biliary Drainage

( Kenji Yamao ),( Kazuo Hara ),( Nobumasa Mizuno ),( Akira Sawaki ),( Susumu Hijioka ),( Yasumasa Niwa ),( Masahiro Tajika ),( Hiroki Kawai ),( Shinya Kondo ),( Yasuhiro Shimizu ),( Vikram Bhatia ) The Editorial Office of Gut and Liver 2010 Gut and Liver Vol.4 No.s1

Endoscopic ultrasonography (EUS) combines endoscopy and intraluminal ultrasonography, and allows imaging with a high-frequency transducer over a short distance to generate high-resolution ultrasonographic images. EUS is now a widely accepted modality for diagnosing pancreatobiliary diseases. EUS-guided fineneedle aspiration (EUS-FNA) using a curved linear- array echoendoscope was initially described more than 20 years ago, and since then many researchers have expanded its indications to sample diverse lesions and have also used it for various therapeutic purposes. EUS-guided biliary drainage (EUS-BD) is one of the therapeutic procedures that has been developed using a curved linear-array echoendoscope. Technically, EUS-BD includes rendezvous techniques via transesophageal, transgastric, and transduodenal routes, EUS-guided choledochoduodenostomy (EUS-CDS), and EUS-guided hepaticogastrostomy (EUS-HGS). Published data have demonstrated a high success rate, albeit with a comparatively high rate of nonfatal complications for EUS-CDS and EUS-HGS, and a comparatively low success rate with a low complication rate for the rendezvous technique. At present, these procedures represent an alternative to surgery or percutaneous transhepatic biliary drainage (PTBD) for patients with obstructive jaundice when endoscopic biliary drainage (EBD) has failed. However, these procedures should be performed in centers with extensive experience in linear EUS and therapeutic biliary ERCP. Large prospective studies are needed in the near future to establish standardized EUS-BD procedures as well as to perform controlled comparative trials between EUS-BD and PTBD, between rendezvous techniques and direct-access techniques (EUS-CDS and EUS-HGS), and between EBD and EUS-BD. (Gut Liver 2010;4(Suppl. 1):S67-75)