Characterization of microRNAs regulating cyclooxygenase-2 gene expression

Sena Yoon,최영철,Yujin Lee,Minho Jin,정용수,윤재승,백광희 한국유전학회 2011 Genes & Genomics Vol.33 No.6

Cyclooxygenase 2 (COX-2) is an enzyme for catalyzing the biosynthesis of prostanoids including prostaglandins and thromboxanes. In this study, miRNA-mediated regulation of COX-2 expression was investigated by employing 9 miRNAs (miR-26a, -101a, -143, -144, -145, -199a, -199a^*, -542-3p,-543). Western blot analysis revealed that ectopic expression of miR-26a, -145, -199a, -542-3p, and -543 significantly reduced the level of COX-2 protein. The inhibitory effect of miR-143, -542-3p, and -543 on COX-2 expression appeared to be mediated via interaction with predicted binding sites in the 3’-untranslated region (3’-UTR) of COX-2 as revealed by luciferase reporter assay. In IL-1β-treated A549 cells, the production of prostaglandin E_2 (PGE_2) was significantly inhibited by miR-26a, -145, -199a, -542-3p, and -543. Together, our findings contribute to the understanding of post-transcriptional regulation of COX-2 and suggest an important role for miRNAs in COX-2 over-expression during inflammation and tumorigenesis.

Efficiency of Greenhouse Evaluation for Selectjng Alternaria Leaf Blight (ALB) Resistant in Carrot

Sena Yoon,Heung Tae kim 한국원예학회 2017 한국원예학회 학술발표요지 Vol.2017 No.5

Induction of growth arrest by miR-542-3p that targets survivin

Yoon, Sena,Choi, Young-Chul,Lee, Suman,Jeong, Yongsu,Yoon, Jaeseung,Baek, Kwanghee Elsevier 2010 FEBS letters Vol.584 No.18

<P><B>Abstract</B></P><P>Survivin is a protein which functions as a mitotic regulator as well as apoptosis inhibitor. In this study, we show that introduction of synthetic miR-542-3p mimetic reduced both mRNA and protein levels of survivin. In A549 cells, luciferase reporter assay revealed that miR-542-3p targeted predicted binding sites in the 3′-untranslated region (3′-UTR) of survivin. We also demonstrate that ectopic expression of miR-542-3p inhibited cell proliferation by inducing Gap 1 (G1) and Gap 2/Mitosis (G2/M) cell cycle arrest. Collectively, these results suggest that survivin is a direct target of miR-542-3p and growth inhibition by miR-542-3p may have a potential utility as an anti-cancer therapy.</P>

Inhibition of Cell Proliferation and Migration by miR-509-3p That Targets CDK2, Rac1, and PIK3C2A

Yoon, Sena,Han, Eunji,Choi, Young-Chul,Kee, Honghwan,Jeong, Yongsu,Yoon, Jaeseung,Baek, Kwanghee Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.4

CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3'-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy.

Application of Rapid and Reliable Detection of Cymbidium Mosaic Virus by Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral Flow Immunoassay

Do-Hyun Kim(Do-Hyun Kim),Rae-Dong Jeong(Rae-Dong Jeong),Sena Choi(Sena Choi),Ho-Jong Ju(Ho-Jong Ju),Ju-Yeon Yoon(Ju-Yeon Yoon) 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Cymbidium mosaic virus (CymMV) is one of economically important viruses that cause significant losses of orchids in the world. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay combined with a lateral flow immunostrip (LFI) assay was developed for the detection of CymMV in orchid plants. A pair of primers containing fluorescent probes at each terminus that amplifies highly specifically a part of the coat protein gene of CymMV was determined for RT-RPA assay. The RT-RPA assay involved incubation at an isothermal temperature (39°C) and could be performed rapidly within 30 min. In addition, no cross-reactivity was observed to occur with odontoglossum ringspot virus and cymbidium chlorotic mosaic virus. The RT-RPA with LFI assay (RT-RPA-LFI) for CymMV showed 100 times more sensitivity than conventional reverse transcription polymerase chain reaction (RT-PCR). Furthermore, the RT-PCR-LFI assay demonstrated the simplicity and the rapidity of CymMV detection since the assay did not require any equipment, by comparing results with those of conventional RT-PCR. On-site application of the RT-RPA-LFI assay was validated for the detection of CymMV in field-collected orchids, indicating a simple, rapid, sensitive, and reliable method for detecting CymMV in orchids.

Inhibitory Effects of Pepper Mild Mottle Virus Infection by Supernatants of Five Bacterial Cultures in Capsicum annuum L.

Venkata Subba Reddy Gangireddygari(Venkata Subba Reddy Gangireddygari ),In-Sook Cho(In-Sook Cho),Sena Choi(Sena Choi),Ju-Yeon Yoon(Ju-Yeon Yoon) 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Pepper mild mottle virus (PMMoV), one of the most prevalent viruses in chili pepper (Capsicum annuum L.) is a non-enveloped, rod-shaped, single-stranded positive-sense RNA virus classified in the genus Tobamovirus. The supernatants of five bacterial cultures (Pseudomonas putida [PP], Bacillus licheniformis [BLI], P. fluorescens [PF], Serratia marcescens [SER], and B. amyloliquifaciens [BA]) were analyzed to find novel antiviral agents to PMMoV in chili pepper. Foliar spraying with supernatants (1:1, v/v) obtained from Luria-Bertani broth cultures of PP, BLI, PF, SER, and BA inhibited PMMoV infection of chili pepper if applied before the PMMoV inoculation. Double-antibody sandwich enzyme-linked immunosorbent assay showed that treatments of five supernatants resulted in 51-66% reductions in PMMoV accumulation in the treated chili pepper. To identify key compounds in supernatants of PP, BLI, PF, SER, and BA, the supernatants were subjected to gas chromatography-mass spectrometry. The 24 different types of compounds were identified from the supernatants of PP, BLI, PF, SER, and BA. The compounds vary from supernatants of one bacterial culture to another which includes simple compounds— alkanes, ketones, alcohols, and an aromatic ring containing compounds. The compounds triggered the inhibitory effect on PMMoV propagation in chili pepper plants. In conclusion, the cultures could be used to further conduct tissue culture and field trial experiments as potential bio-control agents.

하악 전치부에서의 일체형 임플란트 식립 후 즉시부하

윤세나,Yoon, Sena 대한심미치과학회 2018 Journal of the Korean Academy of Esthetic Dentistr Vol.27 No.2

하악 전치부는 다른 구강 내 영역에 비해 높은 임플란트 성공률과 더 나은 접근성을 갖고 있다. 비록 부적절한 드릴링으로 인한 설측 피질골 천공 가능성과 설동맥 손상으로 인한 출혈 가능성이 있더라도 다른 부위와 비교했을 때 주요 해부학적 구조물이 적기 때문에 상대적으로 안전하다. 또한 상악 전치부와 비교했을 때 환자들이 심미적으로 덜 민감한 부위이다. 그러나 좁은 협설측 치조골 폭 때문에 이상적인 임플란트 식립 위치로의 식립은 상악 전치부만큼 어렵다. 이러한 하악 전치부에서 일체형 임플란트는 매우 유용한 치료방법이다. 하악 전치의 해부학적 치근형태와 얇은 치조골을 고려할 때, 임상적으로 3mm보다 큰 직경의 임플란트 식립은 어렵다. 본 증례는 하악 전치부 무치악 부위에서 일체형 임플란트의 식립과 즉시 부하를 동반하여 심미적인 보철물을 구현한 경우이다. Mandibular anterior region has high implant survival rates and better accessibility compared with any other region. Even if there are possibilities for perforation on lingual cortical bone due to improper drilling and bleeding caused by lingual artery damage, mandibular anterior region is a safe region because less amount of major anatomical structures exist compared with other regions. However, because of narrow bucco-lingual width of alveolar ridge, it is challengeable to obtain esthetic implant prosthesis. Although patients are less sensitive subjectively, mandibular anterior region is as difficult as maxillary anterior region in that implant placement location plays a critical role on the prognosis of implant prosthesis. One-piece implant is a very useful option for mandibular anterior region. Considering the narrow roots and thin alveolar bone of mandible, it is clinically difficult for implant diameter to be greater than 3mm In this case, we could approach the esthetic restoration in mandibular anterior region with one-piece implant and immediate loading.

Peroxiredoxin-3 Is Involved in Bactericidal Activity through the Regulation of Mitochondrial Reactive Oxygen Species

Lee, Sena,Wi, Sae Mi,Min, Yoon,Lee, Ki-Young 한국조명·전기설비학회 2016 한국조명·전기설비학회 학술대회논문집 Vol. No.

<P>Peroxiredoxin-3 (Prdx3) is a mitochondrial protein of the thioredoxin family of antioxidant peroxidases and is the principal peroxidase responsible for metabolizing mitochondrial hydrogen peroxide. Recent reports have shown that mitochondrial reactive oxygen species (mROS) contribute to macrophage-mediated bactericidal activity in response to Toll-like receptors. Herein, we investigated the functional effect of Prdx3 in bactericidal activity. The mitochondrial localization of Prdx3 in HEK293T cells was confirmed by cell fractionation and confocal microscopy analyses. To investigate the functional role of Prdx3 in bactericidal activity, Prdx3-knockdown (Prdx3<SUP>KD</SUP>) THP-1 cells were generated. The mROS levels in Prdx3<SUP>KD</SUP> THP-1 cells were significantly higher than those in control THP-1 cells. Moreover, the mROS levels were markedly increased in response to lipopolysaccharide. Notably, the <I>Salmonella enterica</I> serovar Typhimurium infection assay revealed that the Prdx3<SUP>KD</SUP> THP-1 cells were significantly resistant to S. Typhimurium infection, as compared with control THP-1 cells. Taken together, these results indicate that Prdx3 is functionally important in bactericidal activity through the regulation of mROS.</P>

Inhibitory Effects of α-Lipoic Acid on Oxidative Stress-Induced Adipogenesis in Orbital Fibroblasts From Patients With Graves Ophthalmopathy

Hwang, Sena,Byun, Jung Woo,Yoon, Jin Sook,Lee, Eun Jig Wolters Kluwer Health 2016 Medicine Vol.95 No.2

<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>A choice of the optimal treatment for Graves ophthalmopathy (GO) is a challenge due to the complexity of the pathogenesis. Alpha-lipoic acid (ALA) is well known as a multifunctional antioxidant, helping to protect cells against oxidative stress and inflammatory damage.</P><P>The aim of this study was to investigate the effects of ALA on intracellular production of reactive oxygen species (ROS), inflammation, and adipogenesis using primary cultured orbital fibroblasts from patients with GO.</P><P>Intracellular ROS levels and mRNA expressions of proinflammatory cytokines and chemokines including intercellular adhesion molecule-1 (ICAM-1), interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, and regulated upon activation normal T cell expressed and presumably secreted (RANTES) were measured. After adipogenesis, the expressions of peroxisome proliferator-activated receptor (PPAR)γ, CCAAT-enhancer-binding proteins (C/EBP)α and β, and heme oxygenase-1 (HO-1) were investigated.</P><P>H<SUB>2</SUB>O<SUB>2</SUB> dose-dependently stimulated ROS production and HO-1 expression. Addition of ALA strongly attenuated ROS production and further increased HO-1 expression. However, by pretreatment of zinc protoporphyrin (ZnPP), HO-1 inhibitor, ALA inhibition of ROS generation by H<SUB>2</SUB>O<SUB>2</SUB> was abolished. Tumor necrosis factor (TNF)α-induced mRNA expressions of ICAM-1, IL-6, MCP-1, and RANTES were inhibited by ALA treatment. In this context, TNFα-induced phosphorylation of P65 was also inhibited. In addition, ALA dose-dependently inhibited H<SUB>2</SUB>O<SUB>2</SUB>-induced intracellular accumulation of lipid droplets. The expression of adipogenic transcription factors, including PPARγ, C/EBPα, and β, was also inhibited.</P><P>ALA is a potential therapeutic agent for GO because of the inhibitory effects on ROS production and gene expression of proinflammatory cytokines and chemokines, resulting in prevention of adipose-tissue expansion.</P></▼2>

Inhibition of TRAF6 ubiquitin-ligase activity by PRDX1 leads to inhibition of NFKB activation and autophagy activation

Min, Yoon,Kim, Mi-Jeong,Lee, Sena,Chun, Eunyoung,Lee, Ki-Young Informa UK (TaylorFrancis) 2018 AUTOPHAGY Vol.14 No.8

<P>TRAF6 (TNF receptor associated factor 6) plays a pivotal role in NFKB activation and macroautphagy/autophagy activation induced by TLR4 (toll like receptor 4) signaling. The objective of this study was to determine the functional role of PRDX1 (peroxiredoxin 1) in NFKB activation and autophagy activation. PRDX1 interacted with the ring finger domain of TRAF6 and inhibited its ubiquitin-ligase activity. The inhibition on TRAF6 ubiquitin-ligase activity by PRDX1 induced the suppression of ubiquitination of an evolutionarily conserved signaling intermediate in Toll pathways (ECSIT) essential for NFKB activation and BECN1 (beclin 1) required for autophagy activation. An inhibitory effect of PRDX1 on TRAF6 was clearly evidenced in PRDX1-knockdown (PRDX1KD) THP-1, PRDX1KD MDA-MB-231, and PRDX1KD SK-HEP-1 cells. PRDX1KD THP-1 cells showed increases of NFKB activation, pro-inflammatory cytokine production, NFKB-dependent gene expression induced by TLR4 stimulation, and resistance against Salmonella typhimurium infection. Additionally, migration and invasion abilities of PRDX1KD MDA-MB-231 and PRDX1KD SK-HEP-1 cancer cells were significantly enhanced compared to those of control cancer cells. Taken together, these results suggest that PRDX1 negatively regulates TLR4 signaling for NFKB activation and autophagy functions such as bactericidal activity, cancer cell migration, and cancer cell invasion by inhibiting TRAF6 ubiquitin-ligase activity.</P>