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Chlamydia Pneumoniae에 대한 헌혈자, 혈액화학검사자 및 Mycoplasma Pneumoniae 항체 검사자의 혈청 항체 보유율
윤갑준,조상래,권오헌,이경원,정윤섭,김현숙 대한감염학회 1993 감염 Vol.25 No.2
Chlamydia pneumoniae infections, known to be very prevalent in many countries, are not reported in Korea to the best of our knowelege. Prevalence of antibodies against C. pneumoniae was determined by the micro-immunofluorescence test. The proportion of sera with antibody titers of ≥16,tested by polyvalent conjugate of IgG, IgM, and IgA (poly-Ig) were blood donor 76.8%, blood chemistry-tested patients 58.0% and Mycoplasma Pneumoniae antibody-tested patients 22.0%. The positive rates by age groups were ≤years 13.5%, 10-19 years 66.7% and over all 49.6%. Only three sera showed IgM antibody titer of ≥, which became negative after absorption with RF-absorbent. Poly-Ig against C. trachomatis was less prevalent and that against Legionella pneumophila rare. None of the sera at 1:8 dilution were positive for the poly-Ig against Coxiella burnetii. It is concluded that the poly-Ig antibody against C. pneumoniae are highly prevalent in the sera of Koreans suggesting the existance of the infection, and for the diagnosis of the infection, the same interpretation criteria of the MIF titer, which is used in other countries, can also be applied in Korea.
Rapid Typing of Clinical Strains of Mycobacterium tuberculosis by IS6110-based Outward PCR
이혜영,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.2
Worldwide, tuberculosis remains one of the leading infectious diseases, accounting for nearly 3 million deaths and more than 8 million new cases annually. DNA typing of Mycobacterium tuberculosis is important for the control of tuberculosis, since it can be used to track transmission route of tuberculosis, source of internal laboratory contaminations, and to answer questions on the nature of tuberculosis infections such as reactivation or exogenous reinfection of disease. At present, IS6110-based RFLP is the choice of method for typing large numbers of clinical isolates of M. tuberculosis, since it has the highest resolution power. However, RFLP requires long time, high cost and qualified experts, so only reference level laboratories can use the RFLP technique. In order to have an optional molecular typing method suitable for the clinical settings, this study evaluated the use of one of PCR-based typing methods, IS6110-based outward PCR for typing clinical isolates of M. tuberculosis. In brief, the results from this study showed that IS6110-based RFLP is useful to discriminate diverse clinical isolates of M. tuberculosis as well as to identify clinical isolates that belong to the same family or cluster groups that have been previously classified by RFLP analysis. In addition, the banding profiles resulted from IS6110-based outward PCR seemed to represent genomic characteristics of M. tuberculosis, since strains belong to the K-family generated unique band that is not present in any other strains but present only in the genome of K-family strains. The IS6110-based outward PCR was also shown to be useful with DNAs isolated directly from liquid cultures indicating this method can be suitable for typing M. tuberculosis in clinical settings.
Detection of Mycobacterium tuberculosis Antigens in Sputum Samples from Tuberculosis Patients
( Sang Nae Cho ),( Sun Hee Baik ),( Sun Park ),( Joon Chang ),( Se Kyu Kim ),( Sung Kyu Kim ),( Won Young Lee ),( Yun Sop Chong ),( Chul Ho Cho ),( Jin Joo Kim ),( Joo Deuk Kim ) 대한결핵 및 호흡기학회 1994 Tuberculosis and Respiratory Diseases Vol.41 No.6
Phenolic Glycolipids and Neoglycoproteins in Detecting Mycobacterial Infections
Cho, Sang-Nae INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1989 YONSEI REPORTS ON TROPICAL MEDICINE Vol.20 No.1
In summary, phenolic glycolipid antigens have been isolated from several mycobacterial species and fully characterized chemically. Each phenolic glycolipid contained unprecedented opportunity of developing serodiagnostic tools for the relevant infection.l Their corresponding neoglycoprotein antigens have made it cheaper to prepare and easier to manipulate in serodiagnostic tools. The significance of using these native and neoglycoprotein antigens in early detection of mycobacterial infection look promising.
Cho, Sang-Nae,Kim, Hee-Jin,Lee, Hye-Young,Kim, Seung-Chul,Kim, Joo-Deuk The Korea Society for Microbiology 1999 大韓微生物學會誌 Vol.34 No.6
The 38 kDa protein of Mycobacterium tuberculosis, which was known previously as antigen 5, has been extensively used in the serodiagnosis of tuberculosis. In an attempt to develop and evaluate a serodiagnostic test using the antigen, we expressed the 38 kDa protein in BCG and its seroreactivity was compared to that expressed in Escherichia coli. The coding region of the 38 kDa protein was amplified by PCR, and the gene was cloned into a Mycobacterium-E. coli shuttle expression vector pYMC-his and pQE30 expression vector and expressed in BCG and E. coli, respectively. Both recombinant 38 kDa proteins showed strong seroreactivity against pooled serum from tuberculosis patients. There was no significant difference in seroreactivity between the two recombinant antigens in sera from the far advanced tuberculosis patients. However, of 25 tuberculosis patients graded as "minimal" by chest X-ray, 5 (20.0%) were seropositive by r38 kDa expressed in E. coli, while 8 (32.0%) by that expressed in BCG. Likewise, higher seroreactivity by r38 kDa expressed in BCG was found in sera from the moderately advanced tuberculosis. This study thus indicates that the recombinant 38 kDa expressed in BCG is more effective than that expressed in E. coli in detecting antibodies to the native 38 kDa protein of M. tuberculosis in sera from minimally affected tuberculosis patients.