H9 Induces Apoptosis via the Intrinsic Pathway in Non-Small-Cell Lung Cancer A549 Cells

( Sae Bom Kwon ),( Min Je Kim ),( Sun Young Ham ),( Ga Wan Park ),( Kang Duk Choi ),( Seung Hyun Jung ),( Do Young Yoon ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3

H9 is an ethanol extract prepared from nine traditional/medicinal herbs. This study was focused on the anticancer effect of H9 in non-small-cell lung cancer cells. The effects of H9 on cell viability, apoptosis, mitochondrial membrane potential (MMP; Δψm), and apoptosisrelated protein expression were investigated in A549 human lung cancer cells. In this study, H9-induced apoptosis was confirmed by propidium iodide staining, expression levels of mRNA were determined by reverse transcriptase polymerase chain reaction, protein expression levels were checked by western blot analysis, and MMP (Δψm) was measured by JC- 1 staining. Our results indicated that H9 decreased the viability of A549 cells and induced cell morphological changes in a dose-dependent manner. H9 also altered expression levels of molecules involved in the intrinsic signaling pathway. H9 inhibited Bcl-xL expression, whereas Bax expression was enhanced and cytochrome C was released. Furthermore, H9 treatment led to the activation of caspase-3/caspase-9 and proteolytic cleavage of poly(ADPribose) polymerase; the MMP was collapsed by H9. However, the expression levels of extrinsic pathway molecules such as Fas/FasL, TRAIL/TRAIL-R, DR5, and Fas-associated death receptor were downregulated by H9. These results indicated that H9 inhibited proliferation and induced apoptosis by activating intrinsic pathways but not extrinsic pathways in human lung cancer cells. Our results suggest that H9 can be used as an alternative remedy for human non-small-cell lung cancer.

가상 캐릭터를 활용한 음악 감상 경험 증진에 관한 연구

권새봄(Sae Bom Kwon),김향숙(Hyang Sook Kim),김주섭(Jusub Kim) 한국HCI학회 2017 한국HCI학회 학술대회 Vol.2017 No.2

본 연구에서는 비전문가 집단의 음악 감상 경험 증진을 위해, 음악을 잘 이해하는 연기자의 표정을 캡쳐하여 입힌 캐릭터가 음악과 동시에 제공될 경우 감상자의 감정 유발에 유의미한 효과를 가지는지 알아보았다. 실험을 통해, 선별된 클래식 음악에 맞춰 사전에 해당 음악과 친숙한 연기자의 표정을 입힌 가상 캐릭터를 제작해 음악과 함께 제공하였고, 이러한 시각 정보가 감상자들의 감정 유발에 유의미한 영향을 미친다는 것을 발견하였다. 본 연구는 콘서트 현장에서 가상 캐릭터를 활용한 새로운 공연 예술 형식의 가능성을 제시한다는 점에서 의의를 가진다.

해외 1회용 비닐봉투 금지 정책 사례와 우리나라에의 시사점 연구

권새봄(Sae-Bom, Kwon),윤순진(Sun-Jin, Yun) 한국환경정책학회 2017 한국환경정책학회 학술대회논문집 Vol.2017 No.6

Polyphasic Microbial Analysis of Traditional Korean Jeung-Pyun Sourdough Fermented with Makgeolli

( Sae Bom Lim ),( Jagan Mohan Rao Tingirikari ),( Ye Won Kwon ),( Ling Li ),( Grace E. Kim ),( Nam Soo Han ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.2

Jeung-pyun, a fermented rice cake, is prepared by fermenting rice sourdough using makgeolli, a traditional Korean rice wine, in the presence of yeast and lactic acid bacteria (LAB). The goal of this study was to conduct biochemical and microbial analyses of five different rice sourdoughs, each fermented with a different commercial makgeolli, using culture-dependent and culture-independent approaches. All sourdough samples fermented with different makgeolli for 6.5 h showed different profiles in pH, total titratable acidity, organic acid concentration, and microbial growth. LAB belonging to different genera were identified based on colony morphology on modified MRS and sourdough bacteria agar medium. PCRdenaturing gradient gel electrophoresis analyses of the five sourdoughs showed different bands corresponding to LAB and yeast. 16S/26S rRNA gene sequence analyses of the samples confirmed that the predominant LAB in the five fermented rice doughs was Lactobacillus plantarum, Lb. pentosus, and Lb. brevis. Various other Lactobacillus spp. and Saccharomyces cerevisiae were common in all five fermented samples. This study provides comprehensive and comparative information on the microflora involved in fermentation of rice sourdough and signifies the need to develop effective starters to enrich the quality of jeung-pyun.

가압조건의 마이크로웨이브 추출에서 몇가지 인삼성분의 추출특성 모니터링

이새봄(Sae-Bom Lee),이기동(Gee-Dong Lee),정용진(Yong-Jin Jeong),김정숙(Jeong-Sook Kim),권중호(Joong-Ho Kwon) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.28 No.5

천연물로부터 목적성분을 신속하게 추출할 수 있는 가압조건의 마이크로웨이브 추출방법을 이용하여 가용성 인삼성분의 추출에 따른 추출물의 페놀성 화합물 함량, 갈색도 및 전자공여작용의 추출특성을 반응표면분석에 의해 모니터링하였다. 총 페놀성 화합물의 함량에 대한 최대값은 에탄올 농도 67.88%, 추출온도 145℃, 추출시간 6.24분에서 21.65㎎/100ml로 나타났다. 총 페놀성 화합물의 함량은 추출온도가 높고 에탄올 농도가 높을수록 증가하였다. 갈색도는 에탄올 농도 67.21%, 추출온도 147℃, 추출시간 6.02분에서 가장 높은 수치를 나타내었고, 추출온도가 높을수록 증가하였다. 전자공여작용에 대한 최대값은 에탄올 농도 54.33%, 추출온도 147℃, 추출시간 6.11분에서 24.50 units이었다. 전자공여작용은 추출온도가 높을수록 증가하였으며, 에탄올 농도가 50~65% 범위에서 가장 높았다. Microwave extraction system equipped with closed vessels, which is known to rapidly extract target compounds from natural products, was applied to monitor the changes in phenolic compounds, browning color intensity and electron donating ability by using response surface methodology(RSM). Maximum content of phenolic compound was 21.65㎎/100ml in 67.88% of ethanol concentration, 145℃ of extraction temperature, and 6.24 min of extraction time. The phenolic compounds in extracts are dependent on the increase of the extraction temperature and the ethanol concentration. Browning color intensity, which was maximized in 67.21%, 147℃, and 6.02 min, was proportional to the increase of the extraction temperature. Maximum value of electron donating ability was 24.50 units in 54.33%, 147℃, and 6.11 min. The electron donating ability of extracts was dependent on the increase of extraction temperature and maximized in the range from 50 to 65% of ethanol concentration.

가압형 마이크로웨이브 추출장치를 이용한 가용성 인삼성분의 추출조건 최적화

이새봄(Sae-Bom Lee),이기동(Gee-Dong Lee),권중호(Joong-Ho Kwon) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.28 No.2

가압형 마이크로웨이브 추출장치를 이용하여 가용성 인삼성분의 추출특성을 반응표면분석에 의해 모니터링하고 추출조건을 최적화하였다. 가용성 인삼성분의 총 수율은 입자 크기가 작고 추출회수가 많을수록 증가하였다. 그리고 1회 추출시간은 6분 이내가 적합하였으며, 3~4회 반복 추출로써 대부분의 가용성 성분이 추출되는 것으로 나타났다. 추출조건을 최적화하기 위하여 반응표면분석을 실시하여 본 결과, 가용성 인삼성분 및 사포닌 함량의 최적 추출조건은 에탄올 농도 40~50%, 추출온도 140℃ 부근, 추출시간 6분 이내로 나타났다. 이때 가용성 인삼성분은 에탄올 농도가 낮을수록, 사포닌 함량은 에탄올 농도가 높을수록 추출율이 증가하는 경향이었다. 식품공전에 규정되어 있는 추출 온도 90℃ 이하를 제한조건으로 고정하고 추출조건을 예측하여 본 결과는 에탄올 농도 35~50%, 추출시간 3.5~7.5분 범위였으며, 이상의 예측조건에 의한 총수율 및 조사포닌 함량은 실험치와 유사한 값을 나타내었다. Microwave extraction system equipped with closed vessels was applied to confirm its capability of extracting ginseng solubles including saponin, which was monitored to optimize extraction conditions by response surface methodology. Total yield increased with the decrease in particles size and more extraction steps. Soluble components were completely extracted by operating the system within 6 min per one step, which should be repeated by 3 or 4 times. Optimized conditions for maximum extraction of response variables(total yield, crude saponin) were 40~50% of ethanol concentration, about 140℃ of extraction temperature, and within 6 min of the extraction time. The extraction efficiency of total yield was dependent on the decrease of ethanol concentration, while crude saponin content was favored on the higher degree of ethanol concentration. According to the temperature guideline, below 90℃ of extraction temperature, the optimal ranges of extraction conditions were predicted as 30~50% of ethanol concentration and 2.5~6.5 min of extraction time. Estimated values of total yield and crude saponin were in good agreement with experimental values.

Brain Metastases Mimicking Hyperostosis Frontalis Interna on 99mTc HDP Bone Scintigraphy

Kang, Sae-Ryung,Kim, Jahae,Kwon, Seong Young,Min, Jung-Joon,Bom, Hee-Seung,Sohn, Myung-Hee,Song, Ho-Chun Wolters Kluwer Health, Inc. All rights reserved. 2016 Clinical nuclear medicine Vol.41 No.10

<P>Symmetric bifrontal uptake of bone-seeking agents is usually considered as the main feature of hyperostosis frontalis interna in postmenopausal elderly women. This finding is not uncommon in elderly women because of the change in their hormonal level. However, in the present case, a 66-year-old woman with intra-axial brain metastases of breast cancer showed symmetric bifrontal uptake on bone scintigraphy. Therefore, symmetric bifrontal uptake should not always be considered as a definite indicator of hyperostosis frontalis interna. Further evaluation such as SPECT/CT is needed for evaluation of brain metastases especially in cancer patients.</P>

토지이용변화에 따른 비오염물질 부하량 산정을 위한 L-THIA 모형의 적용

손영권 ( Yeong Kwon Son ),윤춘경 ( Chun Gyeong Yoon ),이승재 ( Seung Jae Lee ),이새봄 ( Sae Bom Lee ) 한국물환경학회 2009 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2009 No.-

Evaluation of Drug-Induced Liver Injury Developed During Hospitalization Using Electronic Health Record (EHR)-Based Algorithm

Yewon Kang,Sae-Hoon Kim,So-Young Park,Bo Young Park,Ji-Hyang Lee,Jin An,Ha-Kyeong Won,Woo-Jung Song,Hyouk-Soo Kwon,조유숙,Hee Bom Moon,Ju Hyun Shim,양민석,김태범 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.3

Purpose: The incidence of drug-induced liver injury (DILI) has been increasing; however, few algorithms are available to identify DILI in electronic health records (EHRs). We aimed to identify and evaluate DILI with an appropriate screening algorithm. Methods: We collected data from 3 university hospitals between June 2015 and May 2016 using our newly developed algorithm for identifying DILI. Among patients with alanine transferase (ALT) ≤ 120 IU/L and total bilirubin (TB) ≤ 2.4 mg/dL in blood test results within 48 hours of admission, those who either had 1) ALT > 120 IU/L and TB > 2.4 mg/dL or 2) ALT > 200 IU/L at least once during hospitalization were identified. After excluding patients with liver disease-related diagnosis at discharge, medical records were retrospectively reviewed to evaluate epidemiological characteristics of DILI. Results: The total number of inpatients was 256,598, of whom 1,100 (0.43%) were selected by the algorithm as suspected DILI. Subsequently, 365 cases (0.14% of total inpatients, 95% confidence interval, 0.13–0.16) were identified as DILI, yielding a positive predictive value of 33.1%. Antibiotics (n = 214, 47.2%) were the major class of causative drug followed by chemotherapeutic agents (n = 87, 19.2%). The most common causative drug was piperacillin-tazobactam (n = 38, 8.4%); the incidence of DILI by individual agent was highest for methotrexate (19.4 cases/1,000 patients administered the drug). Common reasons for excluding suspected DILI cases were ischemic hepatitis and postoperative liver dysfunction. Conclusions: Using our EHR-based algorithm, we identified that approximately 0.14% of patients developed DILI during hospitalization. Further studies are needed to modify criteria for more accurate identification of DILI.

수질관리 : 비점오염원 제어를 위한 인공습지의 처리효율분석

손영권 ( Yeong Kwon Son ),윤춘경 ( Chun Gyeong Yoon ),김형철 ( Hyung Chul Kim ),이새봄 ( Sae Bom Lee ),엄한용 ( Han Yong Um ) 대한상하수도학회 2007 공동추계 학술발표회 논문집 Vol.2007 No.-

The field scale experiment was performed to examine the effect of plant coverage on the constructed wetland performance and recommend the optimum development and management of macrophyte communities. Four sets(each set of 0.88ha) of wetland(0.8ha) and pond(0.08ha) systems were used. Water flowing into the Seokmoon estuarine reservoir from the Dangjin stream was pumped into wetland system. Water depth was maintained at 0.3~0.5m and hydraulic retention time was managed to about 2~5days; emergent plants were allowed to grow in the wetlands. After six growing seasons of the construction of wetlands, plant coverage was about 95%, even with no plantation, from bare soil surfaces at the initial stage. Dead vegetation affected nitrogen removal during winter because it is a source of organic carbon which is an essential parameter in denitrification. Biomass harvesting is not a realistic management option for most constructed wetland systems because it could only slightly increase the removal rate and provide a minor nitrogen removal pathway due to lack of organic carbon.