Production of Bioactive 3’-Hydroxystilbene Compounds Using the Flavin-Dependent Monooxygenase Sam5

( Kyung Taek Heo ),( Byeongsan Lee ),( Sangkeun Son ),( Jong Seog Ahn ),( Jae-hyuk Jang ),( Young-soo Hong ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.7

The flavin-dependent monooxygenase Sam5 was previously reported to be a bifunctional hydroxylase with coumarate 3-hydroxylase and resveratrol 3’-hydroxylase activities. In this article, we showed the Sam5 enzyme has 3’-hydroxylation activities for methylated resveratrols (pinostilbene and pterostilbene), hydroxylated resveratrol (oxyresveratrol), and glycosylated resveratrol (piceid) as substrates. However, piceid, a glycone-type stilbene used as a substrate for bioconversion experiments with the Sam5 enzyme expressed in Escherichia coli, did not convert to the hydroxylated compound astringin, but it was converted by in vitro enzyme reactions. Finally, we report a novel catalytic activity of Sam5 monooxygenase for the synthesis of piceatannol derivatives, 3’-hydroxylated stilbene compounds. Development of this bioproduction method for the hydroxylation of stilbenes is challenging because of the difficulty in expressing P450-type hydroxylase in E. coli and regiospecific chemical synthesis.

제주도에서 브루셀라에 의해 발생한 특발성 세균성 복막염

허상택,조유경,김영리,이근화,윤지완,고관수,박미연,최영실 대한감염학회 2007 감염과 화학요법 Vol.39 No.1

Spontaneous bacterial peritonitis is the common infectious disease in liver cirrhosis patients with a fever. Brucellosis is a rare cause of bacterial peritonitis. A case indigenous to a citizen of Jeju, he ate a raw veal of cow four times in last 2 months prior to admission. The gram-negative bacilli were isolated from blood and peritoneal fluid cultures. He was confirmed brucellosis by serologic work- ups. This isolate was confirmed as Brucella abortus by using PCR amplification of 16S ribosomal RNA (rRNA) and omp2. This is the first case of bruellosis that was diagnosed spontaneous bacterial peritonitis in liver cirrhosis patient among native korean citizens. Successful treatment was obtained by using a regimen of doxycycline and gentamicin. Brucella should be suspected as a cause of spontaneous bacterial peritonitis in cirrhotic patients with no response to standard spontaneous bacterial peritonitis treatments. 브루셀라증은 전 세계적으로 발생하는 인수공통 전염병중의 하나로, 국내에서도 해마다 환자가 증가하고 있는 상황이다. 본 증례는 간경화 환자에서 환자의 잘못된 지식을 바탕으로 소 태아 육회를 섭취한 이후 복수와 발열을 주소로 내원한 환자이다. 상기 과거력이 환자의 질환과의 직접적인 연관성을 밝히기는 어려우나 환자의 진단을 추정하는데 도움이 되었으며, 이러한 가능성을 배제하기 위해서라도 국내 유통에 있어서 좀더 적극적인 대체가 필요하다 하겠다. 상기 환자는 브루셀라에 의해 발생한 특발성 세균성 복막염 환자로 제주도에서 발생한 내국인의 첫 증례이기에 문헌 고찰과 함께 보고하는 바이다.

Antioxidative and Antimicrobial Effects of Glycoprotein Isolated from Solanum nigrum Linne

Kye-Taek Lim,Kyung-Sun Heo,Young-Ok Son 한국식품과학회 2002 Food Science and Biotechnology Vol.11 No.5

Selective growth of layered perovskites for stable and efficient photovoltaics

Cho, Kyung Taek,Grancini, Giulia,Lee, Yonghui,Oveisi, Emad,Ryu, Jaehoon,Almora, Osbel,Tschumi, Manuel,Schouwink, Pascal Alexander,Seo, Gabseok,Heo, Sung,Park, Jucheol,Jang, Jyongsik,Paek, Sanghyun,Gar The Royal Society of Chemistry 2018 ENERGY AND ENVIRONMENTAL SCIENCE Vol.11 No.4

<P>Perovskite solar cells (PSCs) are promising alternatives toward clean energy because of their high-power conversion efficiency (PCE) and low materials and processing cost. However, their poor stability under operation still limits their practical applications. Here we design an innovative approach to control the surface growth of a low dimensional perovskite layer on top of a bulk three-dimensional (3D) perovskite film. This results in a structured perovskite interface where a distinct layered low dimensional perovskite is engineered on top of the 3D film. Structural and optical properties of the stack are investigated and solar cells are realized. When embodying the low dimensional perovskite layer, the photovoltaic cells exhibit an enhanced PCE of 20.1% on average, when compared to pristine 3D perovskite. In addition, superior stability is observed: the devices retain 85% of the initial PCE stressed under one sun illumination for 800 hours at 50 °C in an ambient environment.</P>

Anti-oxidative Effects of Glycoprotein Isolated from Solanum nigrum L.

Kye-Taek Lim,Kyung-Sun Heo 한국식품영양과학회 2004 Journal of medicinal food Vol.7 No.3

Glycoprotein from Solanum nigrum L. (SNL glycoprotein) was isolated and tested for anti-oxidative effects on oxygen free radicals using a 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) assay. The free radical scavenging activities of the SNL glycoprotein are optimal in acidic pH and up to 60℃. However, it has minimal activities in the presence of EDTA; although such activities are not dependent on M2+ ions (Ca2+, Mn2+, and Mg2+) in the presence of EDTA. Interestingly, when SNL glycoprotein was treated with deactivation agents (pronase E and NaIO4), the DPPH radical scavenging activity was decreased compared to the SNL glycoprotein treatment alone. The anti-oxidative effects of SNL glycoprotein on superoxide anion and hydroxyl radical under the optimal conditions revealed that SNL glycoprotein has remarkable scavenging effects on both radicals, but exhibited slightly higher scavenging effects on superoxide anion generated by enzymatic hypoxanthine (HX) / xanthine oxidase (XO) system than on hydroxyl radicals generated by the Fenton reaction. However, SNL glycoprotein was more effective against hydroxyl radials in cell cultures (NIH/3T3). Consequently, 20 g/ml SNL glycoprotein has a scavenging ability against superoxide anion corresponding to the ascorbic acid. On the other hand, its hydroxyl radial scavenging corresponds to 0.1 g/ml catalase. From these results, we suggest that SNL glycoprotein has potent anti-oxidative potential.

디자이너와 소비자의 조형요소 인지특성 비교

민경택 ( Kyung Taek Min ),허성철 ( Seong Cheol Heo ) 한국감성과학회 2009 감성과학 Vol.12 No.1

제품 디자인에 있어서 조형화 과정은 실체적인 존재를 만들어 내는 과정이며, 최종적으로 제품에 대한 결과를 이끌어 내는 중요한 과정이다. 이러한 조형화 과정은 일반적으로 디자이너의 주도적인 작업에 의해서 이루어지고 있으며, 이 때 다양한 조형 요소들을 활용하여 결과물을 만들어 낸다. 본 연구에서는 제품 디자인 프로세스에 있어서 제품 형태의 조형화 과정에서 다루어지는 요소들이 소비자와 디자이너의 시각 차이에 의해서 생기는 차이에 대해 알아보고 소비자가 제품의 조형화 과정에 직접적인 참여를 하는 방법과 소비자의 요구가 보다 효율적으로 조형화 과정에 반영되는 디자인 가이드라인의 제시에 대한 가능성을 고찰하고자 하였다. 이를 위하여 먼저 제품 형태를 구성하는 요소들에 대한 정립을 선행하여 기본적인 제품 조형 구성 체계를 마련하였다. 이를 기반으로 기본적인 제품 조형요소와 제품 조형원리를 일부 설정하여 소비자를 대상으로 55개의 컨셉 제품과 64개의 스피커 제품의 이미지에 대한 반응 실험을 진행 하였다. 또한 일부 실험 자극물을 이용하여 디자이너들을 대상으로 동일한 실험을 진행 하였는데, 실험 결과 소비자와 디자이너는 형태의 조형 요소에 대해 어느 정도 시각 차이를 보이며 이는 디자이너의 경우 주관적인 디자인 통념에 의해서, 소비자의 경우 제품에 대한 미숙한 시각적 이해에 의해서 차이가 생기는 것으로 나타났다. 이 결과를 바탕으로 디자이너는 소비자의 최종적인 감성반응을 이끌어내는 디자인 과정에 있어서 형태에 대한 시각 차이를 이해하고, 형태 조형원리를 기반으로 하는 조형요소의 구성을 활용 할 수 있다고 본다. In the process of product design, shaping is the process of making a substantive existence, and ultimately it generates the outcome. The process of shaping is generally led by designer`s initiative work, and in this process, various formative elements are used to generate the outcome. In this research, the basic purposes are to figure out the differences of elements which generated by the differences of consumer`s and designer`s view in the process of shaping of the product, and the characteristics of the affective responses caused by those differences. Also, it will examine how the consumers can directly participate in the process of the shaping of the consumer-participated product, and the feasible guidelines of design in which consumers` needs can be reflected more efficiently to the process of shaping. As a result, consumers and designers have certain degree of difference of view-point about the formative element of the shape. The difference was due to subjective common ideas of design in case of designers, and in case of consumers, it was due to their immature visual understanding. There is another experiment of affective response about the shape of the product. First, I established the sensible image vocabulary based on the shape of the product. And based on the vocabulary, I carried out the same experiments to the consumers and designers.

디자인 조형언어에 대한 소비자의 감성적 인지특성

민경택 ( Kyung Taek Min ),허성철 ( Seong Cheol Heo ) 한국감성과학회 2009 감성과학 Vol.12 No.1

디자인 조형화 과정에 있어서 최근에는 소비자들의 참여가 점차 늘어나고 있는 추세이며, 소비자들은 제품의 형태에 대한 직접적인 평가나 제안을 하기도 하며, 기업에서도 이러한 소비자들의 참여를 유도하기 위한 다양한 방안을 제시하고 있다. 하지만 근본적으로 제품의 형태를 다루는 데에 있어서 소비자와 디자이너는 어느 정도의 시각 차이를 가지고 있으며, 이는 곧 제품 조형화 과정에 있어서 소비자와 디자이너의 효과적인 커뮤니케이션에 방해요소로 작용하고 있다. 이를 해결하기 위해 소비자와 디자이너의 제품 형태에 대한 감성반응에 대한 특징을 통해 소비자의 감성반응을 이끌어 낼 수 있는 효과적인 가이드라인을 알아보는 방법을 연구 하였다. 이러한 소비자와 디자이너의 제품 형태에 대한 감성반응을 알아보기 위한 실험을 진행 하였는데, 먼저 제품 형태 기반의 감성 이미지 어휘에 대한 정립을 진행 하였다. 정립된 이미지 어휘를 기반으로 각각 소비자와 디자이너에게 동일한 실험을 진행 하였다. 실험 결과, 소비자와 디자이너의 형태에 대한 감성 반응은 유의한 성향을 띄게 되는데 이 때 디자이너가 소비자에 비해 보다 극적인 반응을 나타내는 특징이 나타났다. Recently, there is a tendency of consumer`s participation gradually increasing in the design shaping process. Consumers make evaluation or suggestion about the shape of the product, and the industries lay out schemes to elicit consumers` participation. However, when it comes to dealing with the shape of the product, consumer and designer has a fundamental difference in their point of view, and it works as interruption to the efficient communication between the consumer and designer. Therefore, this study will examine the difference of consumer`s and designer`s view of products` shape, and the guidelines of effective molding which elicit the consumers` affective responses. First, I established the sensible image vocabulary based on the shape of the product. And based on the vocabulary, I carried out the same experiments to the consumers and designers. As a result, the affective responses of the two groups toward the shape have similar characteristics and designers` reactions found out to be more dramatic than consumers.

만성발암성 흡입독성시험 우선순위 물질 선정 연구

임경택(Kyung-Taek Rim),임철홍(Cheol-Hong Lim),김현영(Hyeon-Yeong Kim),차신우(Shin-Woo Cha),허용(Yong-Heo),윤진하(Jin-Ha Yoon),김형아(Hyung-A Kim) 한국환경보건학회 2017 한국환경보건학회지 Vol.43 No.1

Objectives: In this study, we seek to perform a priority selection for test substances for chronic inhalation toxicity studies, including acute and subchronic inhalation toxicity studies, which are to be performed after the construction of a chronic/carcinogenicity inhalation toxicity study facility and enactment of pertinent legislation. Methods: Through this study, qualitative and quantitative priority evaluation of test substances according to acute, subchronic and chronic categories were respectively performed and priorities were suggested by expert group review, redundancy and other methods. Meanwhile, a draft on test substance selection criteria, procedures and methods referring to the National Toxicology Program (NTP) system was proposed. Results: This study selected priorities for candidate substances for chronic inhalation toxicity studies to be conducted from 2016. Conclusions: In the future, by assessing in advance the toxicological effects of chemicals to which workers can be potentially exposed in the workplace via long-term inhalation, expected health disturbances among workers will be reduced and it is anticipated that occupational disease induced by chemicals will be effectively prevented.

백운배의 화학성분과 생리활성 효과

허북구(Heo Buk Gu),박용서(Park Yong Seo),박윤점(Park Yun Jum),정규진(Jung Kyoo Jin),조자용(Cho Ja Yong),오경택(Oh Kyung Taek),정웅서(Chung Ung Seo),이경동(Lee Kyung Dong) 한국지역사회생활과학회 2009 한국지역사회생활과학회지 Vol.20 No.4

참다래 과피의 이화학적 성분, 항균활성 및 세포독성

허북구(Heo Buk Gu),박용서(Park Yong Seo),임명희(Im Myung Hee),오경택(Oh Kyung Taek),조자용(Cho Ja Yong) 한국지역사회생활과학회 2009 한국지역사회생활과학회지 Vol.20 No.1