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Exposure data to facial makeup cosmetic products in Korean
( Kyungeun Jung ),( Misoo Choi ),( Seungphil Hong ),( Byungcheol Park ),( Jeonghyun Shin ),( Hakrim Kim ),( Taewon Lee ),( Kyubong Kim ),( Gyeong Hun Park ),( Myunghwa Kim ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2
Backgrounds: Ingredients of cosmetic products (CPs) are able to pass in variable amount across the skin and mucosa. In particular, facial makeup products contain variety of probably hazard compounds such as colorant dyes, heavy metals, preservatives etc. To investigate the exposure data of 10 widely used facial makeup products, realistic exposure assessment study was undertaken. Subjects and method: 358 women and 79 men, aged 15-59 years, who use the facial makeup CPs of interest regularly, were recruited nationwide within 5 metropolitan cities in Korea. Essential information about the amounts of product applied and the frequency of use over a two week period. 10 facial make up CPs including liquid foundation(BB cream), lipstick, eyeliner, eyeshadow, etc.Men were investigated for lip gloss, lip balm, and liquid foundation. The subjects recorded detailed daily usage information over the 2 weeks period. Products were weighed before and after the study period to determine the total amount of product used. Result: 20’s aged women used more lip gloss, lip balm, and eyeliner. 30’s aged women used more eyeshadow. 40-50’s aged women used more amounts of lipstick. The daily use amount of liquid foundation (0.161g), lipstick (0.010g), eye shadow (0.007g) was lower than that of European and American previous studies. The daily useamount of eyeliner (0.006g) was higher than that of European report. Men used similar amount of BB creamand less amount of lip gloss and lip balm comparing with women. Conclusion: We provide provisional database containing exposure information of facial makeup CPs. It can be a fundamental cornerstone for makeup CPs safety assessment.
Neutravidin coated surfaces for single DNA molecule analysis
Kim, Yoori,Jo, Kyubong Royal Society of Chemistry 2011 Chemical communications Vol.47 No.22
<P>We present a novel approach for single DNA molecule analysis using neutravidin coated surfaces. DNA molecules are elongated and reversibly immobilized on neutravidin coated surfaces with pH and salt controls. We demonstrate restriction enzyme reactions for optical mapping and ligation for tethered DNA molecules.</P> <P>Graphic Abstract</P><P>Microfluidic flow dynamically elongates 110 μm DNA biotin-tethered on neutravidin coated surfaces. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0cc05396a'> </P>
Kim Taesoo,Jo Kyubong 한국바이오칩학회 2023 BioChip Journal Vol.17 No.3
Microfl uidics is fl ourishing due to its signifi cant applications in life sciences and biomedical engineering. One of the key challenges in microfl uidics is the manipulation and control of fl uids within microscale channels. Capillary force-driven fl ows provide a potential solution to this challenge by eliminating the need for an external power source. Capillary force- driven fl ows are particularly useful for the reproducible and reliable quantitative analysis of single-molecule DNA. We have designed several microfl uidic devices that employ capillary force to enhance the deposition of DNA molecules onto a positively charged glass surface from a sample solution. The optimization of specifi c dimensions within the microfl uidic device resulted in increased effi ciency of DNA deposition. Shortening the microchannel length reduced fl ow resistance and decreasing the microchannel height enhanced capillary force. Additionally, increasing the outlet reservoir capacity achieves mechanical equilibrium in situations where fl uid fl ow is maximized. These optimizations served to maximize capillary force and improve DNA deposition on the glass surface. The developed device represents an ultra-sensitive platform for quantitative DNA analysis and rapid, accurate point-of-care testing with a minimum detection limit achieved. In conclusion, our work demonstrates the potential of capillary force-driven microfl uidics for the reproducible and effi cient manipulation of fl uids within microscale channels.
Estimation of the critical current of race-track HTS magnet considering angular dependency
Lee, Kyubong,Sim, Kideok,Lee, Changyoung,Kim, Seokho The Korea Institute of Applied Superconductivity a 2015 한국초전도저온공학회논문지 Vol.17 No.3
The high temperature superconducting (HTS) magnet has been developed for the high magnetic field applications such as NMR, MRI and other industrial machinery. In designing process of these HTS magnets, the accurate estimation on the critical current (Ic) is essential to predict and secure the electromagnetic performance. The critical current of 2G HTS tape has anisotropic Ic degradation characteristics with the application of magnetic field - angular dependency of critical current. It is known that the perpendicular magnetic field to the face of HTS tape makes dominant degradation on the critical current for conventional 2G HTS tape. However, recently developed 2G HTS tape has more complex characteristics due to the artificial pinning center. Therefore, the method for Ic estimation reflecting such characteristics of 2G HTS tape needs to be devised. The method considering the angular dependency is introduced in this paper. And the result of newly devised method is compared with that of previous method.
Single-molecule visualization of ROS-induced DNA damage in large DNA molecules
Lee, Jinyong,Kim, Yongkyun,Lim, Sangyong,Jo, Kyubong The Royal Society of Chemistry 2016 The Analyst Vol.141 No.3
<P>We present a single molecule visualization approach for the quantitative analysis of reactive oxygen species (ROS) induced DNA damage, such as base oxidation and single stranded breaks in large DNA molecules. We utilized the Fenton reaction to generate DNA damage with subsequent enzymatic treatment using a mixture of three types of glycosylases to remove oxidized bases, and then fluorescent labeling on damaged lesions via nick translation. This single molecule analytical platform provided the capability to count one or two damaged sites per. DNA molecule (48.5 kb), which were reliably dependent on the concentrations of hydrogen peroxide and ferrous ion at the micromolar level. More importantly, the labeled damaged sites that were visualized under a microscope provided positional information, which offered the capability of comparing DNA damaged sites with the in silico genomic map to reveal sequence specificity that GTGR is more sensitive to oxidative damage. Consequently, single DNA molecule analysis provides a sensitive analytical platform for ROS-induced DNA damage and suggests an interesting biochemical insight that the genome primarily active during the lysogenic cycle may have less probability for oxidative DNA damage.</P>
전기이륜차 겨울철 주행거리 증대를 위한 배터리 열관리에 관한 연구
김호연(Hoyeon Kim),한규봉(Kyubong Han) 한국자동차공학회 2023 한국자동차공학회 부문종합 학술대회 Vol.2023 No.5
In a low-temperature environment, the electrolyte that helps the movement of lithium ions hardens in a battery, and the resulting increase in internal resistance lowers the batterys efficiency. This directly affects the mileage of the electric two-wheeled vehicle in winter. In this paper, we would like to introduce the results of research on the increase in mileage of electric two-wheeled vehicles through use of waste heat generated from vehicles and battery heating.
15kW급 전기 이륜차 냉각 모듈 성능에 관한 실험적 연구
김호연(Hoyeon Kim),한규봉(Kyubong Han) 한국자동차공학회 2023 한국자동차공학회 부문종합 학술대회 Vol.2023 No.5
Electric motorcycle undergo vehicle test to ensure proper performance of the motor and controller under various driving conditions. In the development process, considerable time is required for vehicle production, so predicting the cooling performance of the vehicle through feasibility test in advance can be very helpful in understanding its driving performance. This paper aims to introduce a study on predicting the cooling performance of a 15kW-class electric motorcycle in advance and verifying the vehicles performance comparison.