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Park, Moon-Baik,Ko, Eunjung,Ahn, Changjoon,Choi, Hyun,Rho, Samwoong,Shin, Min-Kyu,Hong, Moo-Chang,Min, Byung-Il,Bae, Hyunsu WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-
Effects of electroacupuncturc (EA) on Th1/Th2 cell response were investigated in BALB/c mice immunized intraperitoneally with 2,4-dinitrophenylated keyhole limpet protein (DNP-KLH). Successive electroacupuncture stimulation on the ST36 acupoint was performed just after immunization. Serum levels of antigen-specific lgE and total lgE were significantly decreased compared with non-acupunctured controls. Production of the Th2-specific cytokines IL-4 and IL-13 in the anti-CD3 mAb-activated splenocytes was significantly suppressed in ST36 electroacupunctured mice compared with non-acupunctured mice. These results imply that successive electroacupuncture on ST36 can decrease the serum level of antigen-specific lgE and total lgE by suppression of the Th2 lineage development.
( Napapan Kangwan ),( Eun Hee Kim ),( Yoon Jae Kim ),( Kwang Hyun Ko ),( Chang Il Kwon ),( Pil Won Park ),( Ki Baik Hahm ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background/aims: Colitis-associated cancer is the one of colorectal cancer that is associated with infi ammatory bowel diseases. Sonic hedgehog (SHH) signaling is essential to normal development of gastrointestinal (GI) tracts and it has been reported that aberrantly activated in GI cancers and regulated cancer stem cells fostering tumorigenesis. We have previously found that colitis-associated cancer can be controlled by anti-infi ammatory intervention and SHH pathway suppression inhibited proliferation of extrahepatic biliary tract cancer. Therefore, the aim of this study was to investigate the SHH pathway contributed to colitis-associated cancer development and the effi - cacy of SHH as preventive agents for colitis-associated cancer. Methods: C57BL/6 mice were induced by treatment with 3 mg/kg azoxymethane(AOM) followed by 2% DSS administration for 7 days. SHh inhibitors were treated by oral gavage and the mice were sacrifi ced at 3 and 16 weeks after AOM injection. Thecerulenin, cyclopamine, and itraconazole were used as SHH inhibitor. IL-6 and TNF-a gene expression were analyzed by RT-PCR. Expression of relative proteins was examined by immunohistochemistry and Western blot. Results: Treatment with SHH inhibitors were restored the length of colon and significantly decreased the number of aberrant crypt foci. In addition, SHH inhibitors were reduced tumor incidence and tumor multiplicity. In tumor tissue, SHH inhibitors not only decreased the infi ammation expression of IL-6, TNF-a, STAT3 and NFκB but also attenuated proliferation, while level of apoptosis markers cleaved caspase 3 was increased. Conclusion: Our results imply that the SHh inhibitor could be an effective strategy to prevent colitis-induced colorectal carcinogenesis through the plausible anti-infi ammatory and anti-tumorigenic actions.
인간 암세포인 AGS와 T24에서의 apoptosis 유도에 미치는 Bacillus subtilis 혈전용해효소 BK-17의 영향
백현(Hyun Baik),서민정(Min Jeong Seo),김민정(Min Jeong Kim),이혜현(Hye Hyeon Lee),강병원(Byoung Won Kang),박정욱(Jeong Uck Park),최영현(Yung Hyun Choi),서권일(Kwon Il Seo),정영기(Yong Kee Jeong) 한국생명과학회 2013 생명과학회지 Vol.23 No.10
인간 암세포의 생육에 미치는 혈전용해효소(BK-1)의 영향을 조사하기 위해, 세포증식, 생존력, 형태변화 및 apoptosis 유도 등을 포함한 여러 가지 생화학적 실험을 하였다. 그 결과, AGS 인간 위장 암세포 및 T24 인간 방광 암세포상에의 BK-17 처리는 그 암세포들의 생존력 및 생율을 농도의존적 방법으로 감소시켰다. 현미경 관찰은, BK-17 처리에 의한 항 생육 효과는 막 수축, 세포의 rounding up, apoptotic bodies와 같은 형태학적 변화를 나타내었다. 특히, RT-PCR과 Western blotting data는, BK-17 처리가 항 apoptosis Bcl-2 군들 특히 Bcl-2, and Bcl-XL의 down-regulation 그리고 AGS 세포에서, apoptosis 촉진 매개체 Bax와 Bad의 up-regulation를 유도했다는 것을 보여주었다. BK-17에 의해 유도된 AGS 세포의 apoptosis는 caspase-3, caspase-8 그리고 caspase-9의 단백질가수분해 활성과 관련이 있었다. 이상의 결과를 볼 때, BK-17은 apoptotic cell death의 유도와 밀접한 관련이 있다는 것을 보여주고 있다. To investigate the effects of a fibrinolytic enzyme, BK-17, on the growth of human cancer cells, we performed various biochemical experiments, including cell proliferation and viability, and investigated subsequent morphological changes and apoptosis induction. BK-17 treatment of AGS human gastric and T24 human bladder carcinoma cells decreased the viability and the proliferation of the cells in a concentration-dependent manner. Microscopic studies indicated that the antiproliferative effects of the BK-17 treatment were associated with morphological changes, such as membrane shrinking, cell rounding up, and the formation of apoptotic bodies, indicating that BK-17 induced apoptosis in the cell lines. Of note, RT-PCR and Western blotting data indicated that the BK-17 treatment induced the down-regulation of antiapoptotic Bcl-2 members, Bcl-2 and Bcl-XL, and the up-regulation of proapoptotic Bax members, Bax and Bad, in the AGS cells. BK-17-induced apoptosis of AGS cells was involved in the proteolytic activation of caspase-3, caspase-8, and caspase-9. Taken together, these findings suggest that BK-17 is associated with the induction of apoptotic cell death.
Si 기판의 소성변형 효과 보정에 의해 개선된 곡률측정법을 이용한 CVD 다이아몬드 박막의 잔류응력 평가
백영준,권동일,정증현,김용협 대한금속재료학회(대한금속학회) 2000 대한금속·재료학회지 Vol.38 No.12
Residual stresses present in diamond films produced by the CVD process often cause cracking and bowing of films, and then degrade product quality. To discuss the causes of bowing and cracking, the measurement and mechanical analyses of residual stresses are very important in diamond film research. Of the residual stresses, the intrinsic stress is one of the most important research topics because its effect on bowing of a free standing diamond film is strong even for its small magnitude. Quantification of the intrinsic stress is limited because high-temperature plastic deformation of Si substrate enlarges the substrate bending. In this study, an analytical model is proposed to obtain the initial intrinsic stress without contribution of the plastic deformation of the substrate. This new model in which the plastic deformation of Si is considered was developed using elastoplastic beam theory and diamond etching technology. In the model, the plastic deformation of Si is given by plastic curvature of substrate measured after removing the diamond film. The experiment is performed on diamond films deposited by microwave plasma assisted CVD. The results show that the overestimated intrinsic stress can be compensated successfully through the new model, which is consistent with that by Raman spectroscopy. The effects of film thickness and deposition temperature on residual stress is discussed in terms of microstructural change of diamond film.
김현수,백순구,권상옥,김춘추,김일회 대한내과학회 2000 대한내과학회지 Vol.58 No.3
Background : In patients with cirrhosis, single large volume paracentesis(SLVP) is an effective and safe treatment for the tense ascites. But the need for routine administration of albumin remains controversial. We investigated the necessity of albumin administration in cirrhosis with tense ascites after SLVP. Methods : 23 patients with cirrhosis with tense ascites were recruited, and examined before and 48 hour after a SLVP. Patients were randomly assigned to be administrated with albumin(6 g/L of ascites removed, n=11) or not(n=12). Systemic and renal hemodynamic parameters(mean arterial blood pressure, cardiac index, systemic vascular resistance index, resistive index of kidney, and serum creatinine), indices associated with sodium homeostasis(urine sodium and osmolarity) and neurohumoral factors such as plasma renin activity and plasma concentration of aldosterone were measured before and 48 hour after a SLVP. Results : There was no significant difference in clinical and laboratory parameters between two groups at entry into the study. Plasma renin activity was significantly increased 48 hour after a SLVP in patients without albumin administration, but the change of plasma renin activity before and after paracentesis(Δplasma renin activity) was not significantly different between two groups. There was no difference in the mean arterial blood pressure, cardiac index, systemic vascular resistance index, resistive index of kidney, serum creatinine, urine sodium and osmolarity and plasma concentration of aldosterone between two groups after SLVP. Conclusion : Single large volume paracentesis without albumin administration is a safe and effective treatment in cirrhosis with tense ascites.(Korean J Med 58:276-282, 2000)