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Bioconverted Orostachys japonicas Extracts Suppress Angiogenic Activity of Ms-1 Endothelial Cells
Lee, Seul Gi,Kim, Jin Soo,Lee, Han-Saem,Lim, Yu-Mi,So, Jai-Hyun,Hahn, Dongyup,Ha, Yu Shin,Nam, Ju-Ock Molecular Diversity Preservation International 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.12
<P>Orostachys japonicus A. Berger (), known as Wa-song in Korea, has been reported to exert various biological effects, such as anti-tumor, anti-oxidant, and anti-febrile effects. However, the anti-angiogenic effects of O. japonicus extracts remain to be investigated. In the present study, we demonstrated the anti-angiogenic effects of bioconverted O. japonicus extract (BOE) in Ms-1 mouse endothelial cells and compared them with the bioactivities of O. japonicus extract (OE). BOE, but not OE, were found to exert anti-angiogenic effects, including inhibition of cell migration, cell adhesion, tube formation of Ms-1 cells, and blood vessel formation of matrigel plug assay in vivo. Furthermore, protein levels of phosphorylated Src kinase were lower in BOE-treated cells than in OE-treated cells. Treatment with OE or BOE did not influence cell viability during the experimental period. Bioconverted extract of O. japonicus have anti-angiogenic effects in vitro and vivo, but non-bioconverted extract do not. We suggest that these observed anti-angiogenic effects are caused by the changes in the composition of bioactive compounds in the extracts as a result of biological conversion.</P>
Han Seul Cho,Woo Joon Park,Go Eun Hong,Ji Han Kim,Min Gu Ju,Chi Ho Lee 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.1
This study was performed to assess the antioxidant activity of Allium hookeri root extract (AHE) on lipid oxidation of raw sulfur-fed pork patties for 14 d of refrigerated storage. Different concentration of ethanol (0-100%) and time (1-12 h) were applied to determine the extraction condition. Water (0% ethanol) extraction for 1 h was selected as an optimal extraction condition of AHE for the following study showing the highest total phenolic content and total flavonoid content, as well as the strongest antioxidant activity. The 1% AHE (SP1), 3% AHE (SP2), and 0.05% ascorbic acid (SP3) were added into sulfur- fed pork patties against controls; SP0 (sulfur-fed pork patties with no AHE) and P0 (normal pork patties with no AHE). The pH values of P0 and SP0 significantly increased (p<0.05) than others on 14 d and redness of P0 showed the largest decrement during storage. P0 and SP0 showed higher production of conjugated dienes on d 7 than others (p<0.05). Thiobarbituric acid reactive substances (TBARS) values were decreased in proportion to the increased level of AHE on 14 d (p< 0.05) resulting in higher TBARS values on P0 and SP0 (p<0.05) and the negative correlation between AHE level and TBARS were also demonstrated (r=-0.910, p=0.001). Therefore, the results suggest that AHE effectively retarded the lipid oxidation rate of sulfur-fed pork patties indicating the potential usage of AHE as a natural preservative.
Lee, Seul Ki,Han, Cheol-Min,Park, Wooram,Kim, Ik Hwan,Joung, Yoon Ki,Han, Dong Keun Elsevier 2019 Materials Science and Engineering C Vol.94 No.-
<P><B>Abstract</B></P> <P>Synthetic biodegradable polymers including poly(lactide-<I>co</I>-glycolide) (PLGA) have been widely used as alternatives to metallic implantable materials in the orthopedic field due to their superior biocompatibility and biodegradability. However, weak mechanical properties of the biodegradable polymers and inflammatory reaction caused by the acidic degradation products have limited their biomedical applications. In this study, we have developed a PLGA composite containing beta-tricalcium phosphate (β-TCP) and magnesium hydroxide (Mg(OH)<SUB>2</SUB>) as additives to improve mechanical, osteoconductivity, and anti-inflammation property of the biopolymer composite simultaneously. The β-TCP has an osteoconductive effect and the Mg(OH)<SUB>2</SUB> has a pH neutralizing effect. The PLGA/inorganic composites were uniformly blended <I>via</I> a twin extrusion process. The mechanical property of the PLGA/β-TCP/Mg(OH)<SUB>2</SUB> composite was improved compared to the pure PLGA. In particular, the addition of Mg(OH)<SUB>2</SUB> suppressed the inflammatory reaction of normal human osteoblast (NHOst) cells and also inhibited the differentiation of pre-osteoclastic cells into osteoclasts. Moreover, synergistically upregulated late osteogenic differentiation of NHOst cells was observed on the PLGA/β-TCP/Mg(OH)<SUB>2</SUB> composite. Taken all together, we believe that the use of β-TCP and Mg(OH)<SUB>2</SUB> as additives with synthetic biodegradable polymers has great potential by the synergistic effect in orthopedic applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PLGA composites containing β-TCP and Mg(OH)<SUB>2</SUB> were successfully fabricated by a twin screw extrusion technique. </LI> <LI> The PLGA/inorganic composites exhibited improved mechanical properties and superior pH neutralization effect. </LI> <LI> PLGA composite showed significantly improved osteogenesis as well as reduced inflammation and osteoclastogenesis. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Seul Chan Park,Seong-Kyoon Choi,Se-Hyeon Han,Song Park,Hye Jin Jeon,Seung Chan Lee,Kyeong Yeon Kim,Young Seo Lee,김지형,Jee Eun Han 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.2
In this study, whiteleg shrimp (Penaeus vannamei) imported from Vietnam were collected from South Korean markets, and examined for 2 viruses: infectious hypodermal and hematopoietic necrosis virus (IHHNV, recently classified as decapod penstyldensovirus-1), and white spot syndrome virus (WSSV). Among 58 samples, we detected IHHNV in 23 samples and WSSV in 2 samples, using polymerase chain reaction and sequencing analyses. This is the first report of IHHNV and WSSV detection in imported shrimp, suggesting that greater awareness and stricter quarantine policies regarding viruses infecting shrimp imported to South Korea are required.
Evaluating feed value of native Jeju bamboo (Sasa quelpaertensis Nakai) for beef cattle
Lee Seul,Baek Youl Chang,Lee Mingyung,Jeon Seoyoung,Bang Han Tae,서성원 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.2
Objective: Recently, indigenous Korean grass Sasa quelpaertensis Nakai (SQ) has garnered much interest as a roughage source for livestock to mitigate its adverse effects on habitat diversity. Thus, the objective of the present study was to evaluate the ruminal fermentation, palatability, and nutrient digestibility of SQ for Korean native beef cattle (Hanwoo) using in vitro rumen fermentation, in situ rumen degradability, and in vivo feeding trials. Methods: Using in vitro tests with rumen fluid as the inoculum for 48 h, ruminal fermentation of SQ was evaluated and compared with that of other roughage sources commonly used in Korea (i.e., rice straw, Timothy hay, and Italian ryegrass [IRG]). Additionally, an in situ trial 96 h was performed using three cannulated Hanwoo steers. Further, an in vivo trial was performed using eight Hanwoo steers to compare the palatability of SQ with rice straw in total mixed ration (TMR) and forage-concentrate separate feeding conditions. Finally, an in vivo digestibility trial of SQ fed as TMR of two particle sizes was performed with four Hanwoo steers. Results: In vitro and in situ trials revealed that SQ was comparable or superior to rice straw in terms of the ruminal fermentation characteristics of pH, gas production, total volatile fatty acid content, and effective ruminal dry matter digestibility (DMD), although its fermentability was lower than that of Timothy hay and IRG. In the palatability test, steers showed a greater preference for SQ when given as TMR. The total tract DMD of SQ fed as TMR was 75.9%±1.37%, and it did not differ by particle size. Conclusion: The feed value of SQ as a roughage source for Hanwoo steers is comparable or superior to that of rice straw, particularly when provided as TMR.
Synthesis of hollow carbon nanostructures as a non-precious catalyst for oxygen reduction reaction
Lee, Seul,Kwak, Da-Hee,Han, Sang-Beom,Hwang, Eui-Tak,Kim, Min-Cheol,Lee, Jin-Yeon,Lee, Young-Woo,Park, Kyung-Won Elsevier 2016 ELECTROCHIMICA ACTA Vol.191 No.-
<P><B>Abstract</B></P> <P>Carbon-based nanostructures as a non-precious catalyst for oxygen reduction reaction (ORR) exhibit excellent electrocatalytic activities due to high specific surface areas and favorable active sites supported by the doped transition metals and nitrogen species. We synthesize the metal-nitrogen doped carbon nanostructures (MPc-C, M=Fe,Co) by using a template method in the presence of metal phthalocyanine including FePc, CoPc, and FeCoPc as metal and nitrogen as well as carbon sources. The as-prepared carbon nanostructures show a high specific surface area, porous structure, and metal-nitrogen dopants, as observed by field-emission scanning electron microscopy (FE-SEM), field-emission transmission electron microscopy (FE-TEM), X-ray photo-electron spectroscopy (XPS), and nitrogen sorption measurement. Especially, among the doped carbon nanostructures, FeCoPc-C exhibits superior ORR electrocatalytic properties in an alkaline medium: an electron transfer number close to 4, high half-wave potential, improved electrochemical stability, and methanol tolerance.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We synthesized the metal-nitrogen doped carbon nanostructures using a facile method. </LI> <LI> The nanostructures consisted of a high specific surface area and porous structure. </LI> <LI> The nanostructures exhibited well-doped transition metal and nitrogen species. </LI> <LI> The nanostructures showed excellent electrocatalytic activities in ORR. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lee, Seul Ah,Moon, Sung-Min,Han, Seul Hee,Hwang, Eun Ju,Park, Bo-Ram,Kim, Jae-Sung,Kim, Do Kyung,Kim, Chun Sung Elsevier 2018 BIOMEDICINE AND PHARMACOTHERAPY Vol.103 No.-
<P><B>Abstract</B></P> <P>Osteoarthritis (OA) is a common degenerative joint disease, characterized by cartilage degradation and inflammation, in the elderly population. <I>Anthriscus sylvestris</I> has been used in Korean traditional medicine and contains many polyphenolic compounds such as cynaroside and chlorogenic acid, which are major active components responsible for its antioxidant effect. In this study, we aimed to evaluate the chondroprotective effect of an aqueous extract of <I>A. sylvestris</I> leaves (AE-ASL) on OA, both <I>in vitro</I> and <I>in vivo</I>. Rat primary chondrocytes were pretreated with AE-ASL for 1 h before interleukin-1β (20 ng/mL) stimulation. The production of nitrite, PGE<SUB>2</SUB>, aggrecan, and collagen type II were detected by Griess reagent and ELISAs. The mRNA levels of iNOS, COX-2, MMP-3, and MMP-13 were measured by RT-PCR. In addition, protein levels of iNOS, COX-2, MMP-3, MMP-13, ADAMTS-4, MAPKs, and NF-κB p65 subunit were measured by western blot analysis. Sulfated glycosaminoglycan (sGAGs) were detected by dimethylmethylene blue (DMMB) assay. During <I>in vivo</I> study, the effects of AE-ASL were evaluated for 8 weeks in a rat model of destabilization of the medial meniscus (DMM) surgery-induced OA. AE-ASL significantly inhibited expression of nitrite, iNOS, PGE<SUB>2</SUB>, COX-2, MMP-3, MMP-13, and ADAMTS-4 in IL-1β-stimulated chondrocytes. Moreover, it decreased the IL-1β-induced degradation of aggrecan, collagen type II, and proteoglycan. In addition, AE-ASL suppressed IL-1β-induced phosphorylation of MAPKs and NF-κB p65 subunit translocation to nucleus. <I>In vivo</I>, AE-ASL inhibited DMM surgery-induced cartilage destruction and proteoglycan loss. Taken together, these results suggest that AE-ASL may be a potential therapeutic agent for the alleviation of OA progression.</P> <P><B>Highlights</B></P> <P> <UL> <LI> AE-ASL inhibits production of inflammation mediator by IL-1β in rat chondrocytes. </LI> <LI> AE-ASL inhibits release of cartilage degrading enzyme by IL-1β in rat chondrocytes. </LI> <LI> Chondroprotective effect of AE-ASL mediated by MAPKs and NF-κB pathway. </LI> <LI> Oral administration of AE-ASL inhibits the cartilage destruction in DMM rat model. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>