http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Eunae You ),( Jangho Jeong ),( Jieun Lee ),( Seula Keum ),( Ye Eun Hwang ),( Jee-hye Choi ),( Sangmyung Rhee ) 생화학분자생물학회 2022 BMB Reports Vol.55 No.4
Cell signals for growth factors depend on the mechanical properties of the extracellular matrix (ECM) surrounding the cells. Microtubule acetylation is involved in the transforming growth factor (TGF)-β-induced myofibroblast differentiation in the soft ECM. However, the mechanism of activation of α-tubulin acetyltransferase 1 (α-TAT1), a major α-tubulin acetyltransferase, in the soft ECM is not well defined. Here, we found that casein kinase 2 (CK2) is required for the TGF-β-induced activation of α-TAT1 that promotes microtubule acetylation in the soft matrix. Genetic mutation and pharmacological inhibition of CK2 catalytic activity specifically reduced microtubule acetylation in the cells cultured on a soft matrix rather than those cultured on a stiff matrix. Immunoprecipitation analysis showed that CK2α, a catalytic subunit of CK2, directly bound to the C-terminal domain of α-TAT1, and this interaction was more prominent in the cells cultured on the soft matrix. Moreover, the substitution of alanine with serine, the 236th amino acid located at the C-terminus, which contains the CK2-binding site of α-TAT1, significantly abrogated the TGF-β-induced microtubule acetylation in the soft matrix, indicating that the successful binding of CK2 and the C-terminus of α-TAT1 led to the phosphorylation of serine at the 236th position of amino acids in α-TAT1 and regulation of its catalytic activity. Taken together, our findings provide novel insights into the molecular mechanisms underlying the TGF-β-induced activation of α-TAT1 in a soft matrix. [BMB Reports 2022; 55(4): 192-197]
You, Eunae,Huh, Yun Hyun,Kwon, Ahreum,Kim, So Hee,Chae, In Hee,Lee, Ok-Jun,Ryu, Je-Hwang,Park, Min Ho,Kim, Ga-Eon,Lee, Ji Shin,Lee, Kun Ho,Lee, Yong-Seok,Kim, Jung-Woong,Rhee, Sangmyung,Song, Woo Keun American Association for Cancer Research 2017 Cancer Research Vol.77 No.17
<P>Disrupting expression of a determinant of microtubule acetylation may pose an effective therapeutic strategy to treat breast cancers.</P><P>Biomechanical remodeling of stroma by cancer-associated fibroblasts (CAF) in early stages of cancer is critical for cancer progression, and mechanical cues such as extracellular matrix stiffness control cell differentiation and malignant progression. However, the mechanism by which CAF activation occurs in low stiffness stroma in early stages of cancer is unclear. Here, we investigated the molecular mechanism underlying CAF regulation by SPIN90 and microtubule acetylation under conditions of mechanically soft matrices corresponding to normal stromal rigidity. SPIN90 was downregulated in breast cancer stroma but not tumor, and this low stromal expression correlated with decreased survival in breast cancer patients. <I>Spin90</I> deficiency facilitated recruitment of mDia2 and APC complex to microtubules, resulting in increased microtubule acetylation. This increased acetylation promoted nuclear localization of YAP, which upregulated expression of myofibroblast marker genes on soft matrices. <I>Spin90</I> depletion enhanced tumor progression, and blockade of microtubule acetylation in CAF significantly inhibited tumor growth in mice. Together, our data demonstrate that loss of SPIN90-mediated microtubule acetylation is a key step in CAF activation in low stiffness stroma. Moreover, correlation among these factors in human breast cancer tissue supports the clinical relevance of SPIN90 and microtubule acetylation in tumor development. <I>Cancer Res; 77(17); 4710–22. ©2017 AACR</I>.</P>
Anti‑acne effects of Castanea crenata bur extract and identification of active compound
You Jiyoung,Ji Hyanggi,Roh Kyung-Baeg,Cho Eunae,Chajra Hanane,Frechet Mathilde,Park Deokhoon,Jung Eunsun 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.1
Acne vulgaris is a common disease of the pilosebaceous unit. Hyperseborrhea, a follicular colonization by Cutibacterium acnes and a complex inflammatory state are pathogenic factors of acne vulgaris. In the present study we investigated the anti-acne efficacy of Castanea crenata bur extract (CBE) in vitro and searched active compound for mitigating hyperseborrhea. In sebocytes, CBE inhibited the sebum synthesis through downregulation of sterol response element-binding protein-1 and peroxisome proliferator-activated receptor γ expression. CBE also inhibited the 5-alpha reductase activity which is associated with androgen-induced sebum production. Moreover, CBE showed anti-inflammatory effect in C. acnes and free fatty acid-induced inflammatory condition through suppressing Toll-like receptor 2 activity. Anti-inflammatory effect was also observed in keratinocytes via inhibition of NF-κB translocation into nuclei. Finally, we identified the ellagic acid as an active compound for inhibiting sebum production in CBE. These findings suggest that CBE have potential to be a multi-target agent for acne vulgaris and a good source of ellagic acid as an anti-sebum compound.
Thirteen-week Repeated Dose Toxicity and Genotoxicity Studies of Sophorae radix
Ji-Ran You,Jeong-Hwan Che,Seung-Hyun Kim,Euna Kwon,Eun-Young Cho,Jung-Hee Yoon,Yun-Soon Kim,Chang-Gil Kang,Hye-Jeong Jeong,Jung Ki Kim,Ja-June Jang,Hee-Chan Kim,Young-Tae Kim,Hyeon-Hoe Kim,Byeong-Cheo 한국실험동물학회 2007 한국실험동물학회 학술발표대회 논문집 Vol.2007 No.-