혈갈 (血竭)의 항산화 및 항염증 효과 = Anti-oxidative and anti-inflammatory effect of fractionated extracts of draconis resina

다국어 초록 (Multilingual Abstract)

Oxidative stress and inflammation are important events in the development of chronic inflammatory diseases including atherosclerosis, diabetes, hypertension, and arthritis. Draconis Resina (DR) has been used as a traditional Korean herbal medicine since ancient times, and today it is used as a medication for wounds, tumors, diarrhea, rheumatism, in the itching of insect bites and with other conditions in the folk medicine. The aim of this study was to determine whether fractionated extracts of DR inhibit free radical generation such as DPPH radical, superoxide radical and nitric oxide, production of nitrite, an index of NO, PGE2, iNOS, COX-2 and proinflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Cytotoxic activity of extracts on RAW 264.7 cells was measured using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. Our results indicated that fractionated extracts, especially dichloromethane and ethylacetate extracts, significantly inhibited free radical generation, the LPS-induced H2O2, NO, PGE2 production and iNOS, COX-2 expression accompanied by an attenuation of TNF-α, IL-1β and IL-6 formation in macrophages. Therefore, these results indicate that dichloromethane and ethylacetate extracts of DR have potential as an agent of chronic inflammatory diseases.

목차 (Table of Contents)

• Ⅰ. 서 론 = 1
• Ⅱ. 실험재료 및 방법 = 3
• 1. 재료 = 3
• 1) 약재 = 3
• 2) 시약 = 4
• 2. 방법 = 5
• 1) Free radical 소거활성 측정 = 5
• 1-1) DPPH radical 소거활성 측정 = 5
• 1-2) Superoxide anions 소거활성 측정 = 5
• 1-3) Nitric oxide 소거활성 측정 = 5
• 2) 세포배양 = 6
• 3) MTS assay = 6
• 4) ROS 생성량 측정 = 6
• 5) NO 생성량 측정 = 7
• 6) PGE2 생성량 측정 = 7
• 7) Western blot analysis = 8
• 8) 세포 배양액 내의 cytokines 측정 = 8
• 3. 통계처리 = 9
• Ⅲ. 실험결과 = 10
• 1. 혈갈 획분층의 free radical 소거활성 측정 = 10
• 1-1. DPPH radical 소거활성 측정 = 10
• 1-2. Superoxide anions 소거활성 측정 = 10
• 1-3. Nitric oxide 소거활성 측정 = 10
• 2. 혈갈 DCM과 EA 추출물의 세포에 대한 독성 = 13
• 3. 세포의 산화적 손상에 대한 보호 효과 = 15
• 4. NO와 PGE2의 생성량에 미치는 영향 = 17
• 5. iNOS와 COX-2의 발현에 미치는 영향 = 20
• 6. 염증성 사이토카인의 생성량에 미치는 영향 = 24
• Ⅳ. 고 찰 = 26
• Ⅴ. 결 론 = 30
• 참고문헌 = 32
• Abstract = 37