DNA回復에 미치는 Poly(ADP-ribose)의 影響에 관한 硏究 = Effects of Poly(ADP-ribose) on DNA Repair

다국어 초록 (Multilingual Abstract)

Effects of 3-aminobenzamide(3-AB), a potent inhibitor of poly(ADP-ribose) polymerase, on DNA replication was determined in CHO cells treated with methyl methanesulfonate(MMS) or UV-light by alkaline s ucrose gradient sedimentation analysis.
MMS has been thought to inhibit the replication intitiation in mammalian cells, but we could also observe the inhibitory effect on chain elongation in CHO cells. At low concentration of 3-AB, the rate of DNA replication was not inbibited but at high concentration was shown in dose dependent manner. When MMS-treated cells were subsquently exposed to 3-AB, additive ingibitory effects were brought out. this result may be due to increases of single stand breaks by 3-AB. In the case of treatment with UV-light and 3-AB, hihg concentration of 3-AB induced the additive inhibitory effect on DNA replication, but low concentration of 3-AB alleviated unexpectedly the inhibitory effects of UV-light alone.

목차 (Table of Contents)

• Ⅰ. 序 論
• Ⅱ. 實驗材料 및 方法
• 1. 細胞培養
• 2. 突然變異原 處理
• 가) 紫外線 處理
• 나) Methyl methanesuefonate 處理
• 다) 적외적 處理
• 라) 알카리 ?糠濃度句配 超遠心分離
• Ⅲ. 結 論
• Ⅳ. 考 察
• 參考文獻
• Summary