대추 메탄올 추출물이 사염화탄소투여에 의한 흰쥐 간 손상에 미치는 영향 = Effect of Jujube Methanol Extract on the Liver Damage in CCl_(4)-treated Rats

다국어 초록 (Multilingual Abstract)

To investigate effects of Jujube methanol extract on the carbon tetrachloride-induced liver damage in rats, experimental animals were divided into 4 groups - control group(CON), Jujube methanol extract administered group(JME), CCl_(4)-treated group(CCL), and Jujube methanol extract and CCl_(4)-treated group(JMC).
Each group was sacrified after 2 and 4weeks of feeding and examined the activities of serum glutamic-oxaloacetic trans-aminase(GOT) and glutamic-pyruvic transaminase(GPT), and the hepatic contents of thiobarbituric acid-reactants(TBARS) and glutathione, and the activities of xanthine oxidase, superoxide dismutase(SOD), catalase and glutathione peroxidase (GPX) in liver.
The activities of serum GOT and GPT, and the hepatic contents TBARS after CCl_(4) administration were markedly increased, compared to CON, but those levels were significantly decreased by the pretreatment of Jujube methanol extract, especially in case of GOT after 2 and 4weeks and TBARS after 4weeks, respectively.
Xanthine oxidase activity which is one of the free radical generating system, was increased by CC4-treatment as compared to CON, but it was inhibited by the treatment of Jujube methanol extract for 2 and 4weeks.
The activities of SOD, catalase and GPX which are related to the free radical scavenging system were elevated by CC4-treatment, compared to CON, and elevated SOD and GPX activities were showed significant decreasing effect by pretreatment of Jujube methanol extract after 2 and 4weeks as compared to CON, however, hepatic catalase activity was not affected significantly.
These results suggest that the inhibition of serum GOT and several activities related to free radical generating and scavenging system elevated by CC4 by the pretreatment of Jujube methanol extract is believed to be a possible protective effect for the carbon tetrachloride-induced hepatotoxicity in rats.

목차 (Table of Contents)

• 목차 = ⅰ
• ABSTRACT = ⅴ
• Ⅰ. 서론 = 1
• Ⅱ. 재료 및 방법 = 5
• 1. 기기 및 시약 = 5
• 1.1. 기기 = 5
• 1.2. 시약 = 5
• 2. 재료 = 6
• 2.1. 시료의 조제 = 6
• 2.2. 실험 동물 처치 = 6
• 3. 효소원의 조제 = 9
• 4. 효소의 활성 측정 = 9
• 4.1. CATALASE 활성도 측정 = 9
• 4.2. LIPID PEROXIDE 함량측정 = 10
• 4.3. GLUTATHIONE PEROXIDASE 활성도 측정 = 11
• 4.4. XANTHINE OXIDASE 활성도 측정 = 12
• 4.5. SUPEROXIDE DISMUTASE 활성도 측정 = 13
• 4.6. GLUTATHIONE 함량측정 = 15
• 4.7. PROTEIN 정량 = 16
• 5. 실험 결과의 통계 처리 = 17
• Ⅲ. 실험 결과 및 고찰 = 18
• 1. 대추 메탄올 추출물이 흰쥐의 혈청 GOT 및 GPT의 활성에 미치는 영향 = 18
• 2. 대추 메탄올 추출물이 흰쥐의 간 TBA 반응성 산물량에 미치는 영향 = 19
• 3. 대추 메탄올 추출물이 흰쥐의 간조직 중 xanthine oxidase, SOD, catalase 및 glutathione peroxidase 활성도에 미치는 영향 = 27
• 4. 대추 메탄올 추출물이 흰쥐의 간 조직 중 glutathi-one 함량 변화에 미치는 영향 = 36
• Ⅳ. 요약 = 39
• 참고문헌 = 40