Vacor 投與白鼠에서의 末梢神經機能障碍 = Functional study on peripheral neuropathy in Vacor-treated Rat

국문 초록 (Abstract)

살서제로 개발된 Vacor가 사람에서 당뇨병을 일으키고, 여러 가지 신경장애 증상을 유발하며, 동물실험에서도 대량의 Vacor 투여로 중추신경계, 말초신경계 및 췌 β세포등에 형태학적 변화를 일으킬 수 있음이 보고되었다.
본 실험에서는 백서를 이용하여 Vacor의 말초신경에 대한 작용을 전기생리학적 및 생화학적으로 검토하였다.
Vacor를 100mg/kg 투여받은 백서는 4시간 후에 혈당치가 상승되었으며 좌골신경을 자극하여 얻은 족저근의 복합근활동전위의 진폭을 저하시켰으나 좌골신경-비복신경의 신경전도 속도에는 영향을 주지않았다. 그리고 Vacor 10mg/kg의 용량을 투여한 군에서는 1주일후에 체중을 감소시켰으며, 신경전도속도에는 영향을 미치지 않았으나 복합근활동전위를 현저히 저하시켰고, 신경내 inositol을 증가시켰다.
이상의 결과로 보아 Vacor에 의한 말초신경장애는 주로 축삭의 변성에 기인된 것으로 사료되며 고용량을 투여한 급성기 뿐만아터라 저용량을 투여한 경우에도 1주일후에는 말초신경장애를 일으키는 것으로 생각되었다.

다국어 초록 (Multilingual Abstract)

Male Wistar rats were given single oral dose of Vacor (1, 10, or 100mg/kg) and studied for effects on conduftion velocity of the sclatic-tibial nerve, and amplitude of compound muscle action potential, the inositol and sorbitol contents, and Na^+, K^+-ATPase activity of the sciatic nerve, and on body weight and plasma glucose levels at various time intervals after administration of the substance.
1. Vacor, four hr after 100mg/kg, p.o., had no effect on body weight but increased plasma glucose levels significantly. The amplitude of the compound muscle action potential decreased but conduction velocity of sciatic-tibial nerve was unchanged. Na, K-ATPase activity and inositol contents of the sciatic nerve, were also unchanged, while sobitol levels were not detectable.
2. Vacor, one week after 10mg/kg, p.o., decreased body weight significantly but did not affect plasma glucose levels. The amplitude of the compound muscle action potential decreased but conduction velocity of sciatic-tibial nerve was unchanged. Sciatic nerve Na, K-ATPase activity was not affected but inositfl content was significantly increased, while sorbitol levels were not detectable.
3. Vacor, 6 hr after 10mg/kg, p.o., had no effect on body weight or plasma glucose levels. The amplitude of the compound muscle action potential and conduction velocity of sciatic-tibial nerve were unchanged, and the Na, K-ATFase activity and inositol and sorbital contents of the sciatic nerve, were not affected.
4. Vacor, one week after 1mg/kg, p.o., had no effect on any of the above mentioned parameters.
It is concluded that high dose of Vacor in acute state as well as low dose in subacute or chronic state cause impairment of peripheral nerve resulted mainly from axonal degeneration.

목차 (Table of Contents)

• 국문초록 = 4
• 서론 = 5
• 실험방법 = 6
• 결과 = 9
• 고찰 = 13
• 참고문헌 = 14
• 영문초록 = 16