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Prevalence of rotavirus diarrhea among hospitalized under-five children
Mathew, M. A.,Paulose, A.,Chitralekha, S.,Nair, M. K.,Kang, G.,Kilgore, P. INDIAN PEDIATRIC 2014 Indian pediatrics Vol.51 No.1
Objectives: To estimate the prevalence of rotavirus diarrhea among hospitalized children less than 5 years of age in Kerala State and to determine the circulating strains of rotavirus in Kerala.Design: Multicenter, cross-sectional study.Setting: Eight representative hospitals in Kunnathunadu Thaluk, Ernakulam district, Kerala.Participants: Children in the age group under 5 yearsMethods: Hospitalized children admitted with acute diarrhea were examined and standardized case report form was used to collect demographic, clinical and health outcome. Stool specimens were collected and ELISA testing was done. ELISA rotavirus positive samples were tested by reverse transcription PCR for G and P typing (CMC Vellore).Results: Among the 1827 children, 648 (35.9%) were positive for rotavirus by the Rotaclone ELISA test. The prevalence of rotavirus diarrhea in infants less than 6 months of age was 24.7%; 6-11 months 31.9%; 12-23 months 41.9%; 24-35 months 46.9%; and 33.3% in 36- 59 months. Rotavirus infections were most common during the dry months from January through May. GIP[8] (49.7%) was the most common strain identified followed by G9P[8] (26.4%), G2P[4] (5.5%), G9P[4] (2.6%) and G12P[6] (1.3%).Conclusions: The prevalence of rotavirus diarrhea among hospitalized children less than 5 years is high in Ernakulam district, Kerala State.
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