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RISS 인기검색어
- 검색키워드
- 저자 : Zhengxiang Ning (검색결과 2 건)
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Antifungal Effect of Triglycerol Monolaurate Synthesized by Lipozyme 435-Mediated Esterification
Song Zhang,Jian Xiong,Wen-Yong Lou,Zhengxiang Ning,Denghui Zhang,Jiguo Yang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4
This study was designed to synthesize triglycerol monolaurate (TGML) with Lipozyme 435 as the catalyst, and explore its effects on the growth of Aspergillus parasiticus (A. parasiticus) and Aspergillus flavus (A. flavus) and the secretion of aflatoxin b1. The highest content of TGML (49.76%) was obtained at a molar ratio of triglycerol to lauric acid of 1.08, a reaction temperature of 84.93°C, a reaction time of 6 h and an enzyme dosage of 1.32%. After purification by molecular distillation combined with the washes with ethyl acetate and water, the purity of TGML reached 98.3%. Through characterization by electrospray-ionization mass spectrometry, infrared spectrum and nuclear magnetic resonance, the structure of TGML was identified as a linear triglycerol combined with lauroyl at the end. Finally, the inhibitory effects of TGML on the growths of A. parasiticus and A. flavus and the secretion of aflatoxin b1 were evaluated by measuring the colony diameter, the inhibition rate of mycelial growth and the content of mycotoxin in the media. The results indicated that TGML had a stronger inhibitory effects on colony growth and mycelial development of both toxic molds compared to sodium benzoate and potassium sorbate, and the secretions of toxins from A. parasiticus and A. flavus were completely suppressed when adding TGML at 10 and 5 mM, respectively. Based on the above results, TGML may be used as a substitute for traditional antifungal agents in the food industry.
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Yilu Bao,Ming Liu,Xia Wu,Wei Cong,Zhengxiang Ning 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
The objective of this study was to estimate the CO2 absorptivity provided by an in situ carbon supply system using a photosynthetic culture of the cyanobacterium Spirulina platensis in an open raceway pond. The effects of initial total carbon concentrations (ranging from 0 to 0.1 mol/L), suspension depths (ranging from 5 to 20cm) and pH values (ranging from 8.9 to 11.0) on the CO2absorptivity were studied. The results indicated that CO2absorptivity was positively correlated with pH value,negatively correlated with total carbon concentration, and only negligibly affected by the suspension depth. The optimum total carbon concentration range and pH range were 0.03 ~ 0.09 mol/L and 9.7 ~ 10.0, respectively. An average CO2 absorptivity of 86.16% and average CO2utilization efficiency of 79.18% were achieved using this in situ carbon-supply system in large-scale cultivation of Spirulina platensis, with an initial total carbon concentration of 0.06 mol/L and pH value of 9.8. Our results demonstrated that this system could obtain a favorable CO2utilization efficiency in outdoor, large-scale cultivation of Spirulina platensis in open raceway ponds.
ScienceON
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