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Circ_0001666 affects miR-620/WNK2 axis to inhibit breast cancer progression
Su Na,Liu Li,He Shan,Zeng Linghai 한국유전학회 2021 Genes & Genomics Vol.43 No.8
Background Circular RNAs (circRNAs) play important roles in the progression of various cancers, including breast cancer (BC). However, the role of circ_0001666 in BC remains unclear. Objective To explore the role of circ_0001666 in the progression of BC and reveal its potential molecular mechanism. Methods Real-time polymerase chain reaction was conducted to determine the expression of circ_0001666, miR-620 and with-no-lysine kinase 2 (WNK2). Cell counting kit 8 assay, fow cytometry and transwell assay were used to measure cell proliferation, apoptosis, migration and invasion. Western blot was utilized to examine the level of protein. Dual-luciferase reporter assay and RNA immunoprecipitation assay were used to verify the interaction between miR-620 and circ_0001666 or WNK2. Mice xenotransplantation models were built to explore the efect of circ_0001666 on BC tumor growth in vivo. Results Circ_0001666 was downregulated in BC tumor tissues and cells. Overexpressed circ_0001666 inhibited the proliferation, migration, invasion, while promoted apoptosis and tumor growth of BC in vitro or in vivo. Furthermore, circ_0001666 could serve as a sponge of miR-620. MiR-620 inhibitor hindered BC cell progression, which was similar to the efect of circ_0001666 overexpression. WNK2 was a target of miR-620, and circ_0001666 could sponge miR-620 to positive regulate WNK2. The knockdown of WNK2 reversed the efect of circ_0001666 overexpression on BC progression. Conclusion Circ_0001666 hindered the progression of BC via miR-620/WNK2 axis.
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