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RISS 인기검색어
- 검색키워드
- 저자 : Yusuf Ozkan (검색결과 3 건)
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Alpha-Amylase Immobilization on Epoxy Containing Thiol-Ene Photocurable Materials
( Cakmakci ),( Emrah ),( Ozkan Danis ),( Serap Demir ),( Yusuf Mulazim ),( Memet Vezir Kahraman ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.2
Thiol-ene polymerization is a versatile tool for several applications. Here we report the preparation of epoxide groups containing thiol-ene photocurable polymeric support and the covalent immobilization of α-amylase onto these polymeric materials. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) coupled with SEM was used to explore the chemical composition. The polymeric support and the immobilization of the enzyme were characterized by FTIR analysis. SEM-EDS and FTIR results showed that the enzyme was successfully covalently attached to the polymeric support. The immobilization efficiency and enzyme activity of α-amylase were examined at various pH (5.0-8.0) and temperature (30-80oC) values. The storage stability and reusability of immobilized α-amylase were investigated. The immobilization yield was 276 ± 1.6 mg per gram of polymeric support. Enzyme assays demonstrated that the immobilized enzyme exhibited better thermostability than the free one. The storage stability and reusability were improved by the immobilization on this enzyme support. Free enzyme lost its activity completely within 15 days. On the other hand, the immobilized enzyme retained 86.7% of its activity after 30 days. These results confirm that α-amylase was successfully immobilized and gained a more stable character compared with the free one.
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Pancreatic Islet Cell Amyloidosis Manifesting as a Large Pancreas
Mehmet Ruhi Onur,Mehmet Yalnız,Ahmet Kursad Poyraz,Ibrahim Hanifi Özercan,Yusuf Ozkan 대한영상의학회 2012 Korean Journal of Radiology Vol.13 No.1
A 39-year-old female patient presented to our hospital with epigastric pain lasting for two months. Laboratory results showed impaired glucose tolerance. Ultrasonography of the patient showed a hypoechoic, diffusely enlarged pancreas. CT revealed a large pancreas, with multiple calcifications. On MRI, a diffusely enlarged pancreas was seen hypointense on both T1- and T2-weighted images with heterogeneous enhancement after gadolinium administration. A biopsy of the pancreas revealed primary amyloidosis of islet cells. Decreased signal on T1-weighted images without inflammation findings on CT and MRI were clues for the diagnosis.
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Emre Uzun,Muhammed Emin Polat,Kazim Ceviz,Erkan Olcucuoglu,Sedat Tastemur,Yusuf Kasap,Samet Senel,Ozkan Ozdemir 대한비뇨의학회 2024 Investigative and Clinical Urology Vol.65 No.1
Purpose: We analyzed the surgical results of patients who were treated and followed up for prostate cancer in our clinic to predict the relationship between periprostatic adipose tissue and patients with and without pathologically upstaged disease. Materials and Methods: The study included patients who had undergone robot-assisted radical prostatectomy and preoperative multiparametric prostate magnetic resonance imaging between 18 February 2019 and 1 April 2022. The patients were divided into two groups, and the surgical and transrectal ultrasound-guided biopsy pathology results were compared according to tumor grade and distribution in 124 patients who met the selection criteria. We analyzed the relationships between upgrading/upstaging and periprostatic adipose tissue thickness (PPATT) and subcutaneous adipose tissue thickness (SATT) as measured in magnetic resonance imaging. Results: The median PPATT was 4.03 mm, whereas the median SATT was 36.4 mm. Upgrading was detected in 45 patients (36.3%), and upstaging was detected in 42 patients (33.9%). A receiver operating characteristic regression analysis revealed that a PPATT >3 mm was a predictive factor for upstaging after radical prostatectomy (area under curve=0.623, 95% confidence interval [CI] 0.519–0.727, p=0.025). Multivariate logistic regression analyses revealed that prostate specific antigen density ≥0.15 ng/mL/cm3 (odds ratio [OR] 5.054, 95% CI 2.008–12.724, p=0.001), International Society of Urological Pathology grade ≥4 (OR 9.369, 95% CI 2.109–21.626, p=0.003) and higher PPATT (OR 1.358, 95% CI 1.081–1.707, p=0.009) were independent risk factors for upstaging after radical prostatectomy. Conclusions: We believe that the PPATT may be a predictive indicator for upstaging after robot-assisted laparoscopic radical prostatectomy.
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