http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Yoshinouchi, Yuka (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Yoshinouchi, Yuka,Shimizu, Sachiko,Lee, Jin-Seon,Hirano, Masashi,Suzuki, Ken-ichi T.,Kim, Eun-Young,Iwata, Hisato Elsevier 2019 Ecotoxicology and environmental safety Vol.181 No.-
<P><B>Abstract</B></P> <P>To assess the effect of exposure to persistent organic pollutants (POPs) on the estrogen receptor (ER) signaling pathway in Baikal seals (<I>Pusa sibirica</I>), we investigated the molecular characterizations and functions of two Baikal seal ER (bsER) isoforms, bsERα and bsERβ. The bsERα and bsERβ cDNA clones isolated have an open reading frame of 595 and 530 amino acid residues, respectively. The tissue distribution analyses of bsER mRNAs showed that bsERα transcripts were primarily found in the ovary and uterus, and bsERβ in the muscle in wild Baikal seals. The immunofluorescence staining assay showed that 17β-estradiol (E<SUB>2</SUB>) treatment promoted the nuclear translocation of <I>in vitro</I>-expressed bsERα. Transient transfection of bsERα in U2OS cells enhanced the transcription of <I>pS2</I>, an ER target gene of E<SUB>2</SUB>. We then measured bsER-mediated transactivation potencies of POPs in an <I>in vitro</I> reporter gene assay system, in which a bsERα or bsERβ expression vector was transfected into COS-1 cells. For comparison, transactivation potencies of POPs on mouse ERs (mERα and mERβ) were also evaluated in the same manner. Results showed significant dose-dependent responses of bsERs and mERs when treated with <I>p,p’</I>-dichlorodiphenyltrichloroethane (<I>p,p’</I>-DDT), and <I>p,p’</I>-dichlorodiphenyldichloroethylene (<I>p,p’</I>-DDE). bsERs and mERs showed no response when exposed to polychlorinated biphenyls (PCBs) or 2,3,7,8-tetrachlorodibenzo-<I>p</I>-dioxin. Comparison of the dose-response curves of DDTs across species (bsERs vs. mERs) showed that bsERα had a response similar to mERα, but bsERβ was less sensitive than mERβ. Comparing the lowest observable effective concentrations of <I>p,p</I>′-DDT (2.8 μM) and <I>p,p</I>′-DDE (10 μM) for <I>in vitro</I> bsERα-mediated transactivation with their hepatic concentrations in wild Baikal seals indicated that some individuals accumulated these compounds at levels comparable to the effective concentrations, suggesting the potential disruption of the bsERα signaling pathway in the wild population by these compounds. Co-transfection experiments with bsER and the aryl hydrocarbon receptor (AHR) suggested that high accumulation of estrogenic compounds exerts a synergistic effect with dioxin-like congeners on ER signaling through AHR activation in the wild seal population.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Baikal seal estrogen receptor cDNA clones (bsERα and bsERβ) were isolated. </LI> <LI> <I>In vitro</I> bsER-expressed reporter gene assay system was constructed. </LI> <LI> Dose-dependent responses of bsERs to <I>p,p</I>′-DDT and <I>p,p</I>′-DDE were observed. </LI> <LI> Aryl hydrocarbon receptor activation by dioxins disrupts bsER signaling pathway. </LI> </UL> </P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
