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Jung, Min Kyung,Park, Yoorim,Song, Seok Bean,Cheon, So Young,Park, Sunyoung,Houh, Younkyung,Ha, Soogyeong,Kim, Hee Jung,Park, Jung Min,Kim, Tae Sung,Lee, Wang Jae,Cho, Byung Joo,Bang, Sa Ik,Park, Hyun The Society for Investigative Dermatology, Inc 2011 The Journal of investigative dermatology Vol.131 No.10
Erythroid differentiation regulator (Erdr1) was first discovered in mouse leukemia cell lines and functions as a stress-related survival factor. This study investigated whether Erdr1 regulates murine melanoma progression, as well as the mechanism involved in Erdr1-regulated metastasis. The expression of Erdr1 is negatively correlated with IL-18 expression, which has a pro-cancer effect in melanoma. To study the role of Erdr1 as an anti-cancer factor, cell migration, invasion, and proliferation were measured. Erdr1 overexpression markedly inhibited the level of cell migration, invasion, and proliferation in B16F10 cells in vitro. In addition, Erdr1 overexpression significantly suppressed melanoma lung colonization, metastasis, and tumor growth in vivo. To identify the factors involved in Erdr1-reduced metastasis, heat shock protein 90 (HSP90), a well-known stress protein and contributor to tumor metastasis, was examined. We found that HSP90 was significantly decreased in Erdr1-overexpressing cells. Functional analysis demonstrated that HSP90 small-interfering RNA transfection reduced the migration ability and metastasis of melanoma. In conclusion, Erdr1 shows a powerful anti-metastasis effect that leads to the ability to reduce the metastatic potential of murine malignant melanoma cells. Erdr1 is an anti-metastatic factor that may be a possible therapeutic target for treatment of melanoma.
Park, Yoorim,Jung, Min Kyung,Yoon, Sun Young,Lee, Ha-Reum,Hur, Dae Young,Kim, Daejin,Yang, Yoolhee,Kim, Tae Sung,Kim, Seonghan,Yoon, Suk Ran,Park, Hyun Jeong,Bang, Sa Ik,Cho, Dae Ho Published for the International Union of Biochemis 2013 Biotechnology and applied biochemistry Vol.60 No.3
<P>Adipose stem cells (ASCs) are pluripotent cells that can generate pure fat tissue for regeneration. Differentiated adipose cells have been generated by a common inducer cocktail composed of dexamethasone, insulin, and isobutylmethylxanthine (DIM). The major drawbacks of adipose cells are their tendency to float on the culture media and their cost. To overcome some of these disadvantages, a new inducer cocktail that includes insulin, dehydroepiandrosterone, and histamine (DH IH) was tested. As a result, lipid accumulation was elevated more than twofold with DH IH than with DIM. Cell adhesion and viability, which are important factors for stable differentiation, were increased with DH IH and were proven through measurement of mRNA expression levels of adhesion marker genes, N-cadherin and vascular cell adhesion molecule, as well as through an alamar blue assay. The expression of adipogenesis-related genes, adiponectin, and glucose transporter type 4 lasted for a long time. To improve the efficiency of grafting, cell adhesion and neovascularization need to be increased. Neovascularization was observed around the transplanted adipose cells, which showed a higher number of vessel formation in DH IH than in DIM. The above results suggest that DH IH can produce pure differentiated adipose cells effectively and enhance their adhesion onto the target location when these differentiated adipose cells were applied as a clinical resource.</P>
정지운(Jiwoon Jung),이태윤(Taeyoon Lee),최유림(Yoorim Choi),조윤민(Younmin Cho),이민구(Mingu Lee) 한국정보기술학회 2021 Proceedings of KIIT Conference Vol.2021 No.6
MZ세대를 중심으로 윤리적 소비를 중시하는 미닝아웃 소비 트렌드와 온라인 소액기부에 대한 긍정적 참여의향이 확산되고 있다. 본 논문에서는 반려동물을 키우고 싶지만 여건이 되지 않아 키우지 못하는 ‘랜선집사’를 위한 온라인 유기동물 후원 플랫폼 서비스를 제안한다. 유기동물 보호센터와 연계한 유기동물 후원 관계 맺기 시스템을 통해 사용자와 후원 동물 간 유대감 형성 및 보호센터 관리의 질 향상을 목적으로 하며, 궁극적으로 사용자의 정서적 만족감 충족과 유기동물의 입양 가능성 증가를 기대한다. Mining-out consumer trends that value ethical consumption and willingness to participate in micro-donations online are spreading among the MZ generation. For "online growers" who wish to have pets but cannot afford to, an online micro-donation platform service for abandoned pets is proposed in this paper. It aims to build a bond between users and abandoned pets, to improve the quality of care for pets, and ultimately to meet users" emotional satisfaction and increase the chance of adoption of abandoned pets.
YOON, SUN YOUNG;LEE, HA REUM;PARK, YOORIM;KIM, JOO HEON;KIM, SOO YOUNG;YOON, SUK RAN;LEE, WANG JAE;CHO, BYUNG JOO;MIN, HYEYOUNG;BANG, JUNG-WOOK;PARK, HYUNJEONG;BANG, SA IK;CHO, DAEHO Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2
Intratumoral hypoxia has been correlated with distant metastatic potential. Two hypoxia inducible factors (HIFs). HIF-1α and HIF-2α, are induced by hypoxia, and high expression of these proteins has been correlated to angiogenesis and distant metastasis. Thymosin β4 (Tβ4) is frequently highly expressed in cancer, and this overexpression correlates with malignant progression. The objective of this study was to investigate the clinical correlation of HIF-α with Tβ4 and the intracellular functional roles of Tβ4 on HIF-α activation. We examined HIF-1α, HlF-2α and Tβ4 expressions in clinical human breast carcinoma (n=70) by immunohisto-chemistry. We show that high expression of HIF-1α and HIF-2α strongly correlates with Tβ4 expression (P≤0.0001) and overexpression of Tβ4 correlates significantly with patients with lymph node metastasis (P<0.05) of human breast cancer. Additionally, we demonstrate that hypoxia up-regulates intracellular Tβ4 protein, which then affects HIF-α activity, which is the key in regulating VEGF expression. We confirmed that hypoxia-induced intracellular Tβ4 and HIF-α activities were reduced by interference of TIM expression using Tβ4 shRNA lentivirus. Vascular epidermal growth factor (VEGF)-A, a well-recognized lymphangiogenic cytokine, was also down-regulated, but VEGF-C and VEGF-D expressions were not affected. These findings suggest that the overexpression of Tβ4 is strongly associated with HIF-1α and HIF-2α expression and is also clinicopathologically involved with lymph node metastatic potential of breast cancer through the modulation of HIF-a activation and induction of VEGF-A. Ultimately, these results highlight Tβ4 as a potentially therapeutic target in malignant cancers.
Yoon, Sun Young,Lee, Ha Reum,Park, Yoorim,Kim, Joo Heon,Kim, Soo Young,Yoon, Suk Ran,Lee, Wang Jae,Cho, Byung Joo,Min, Hyeyoung,Bang, Jung-Wook,Park, Hyunjeong,Bang, Sa Ik,Cho, Daeho National Hellenic Research Foundation 2011 Oncology reports Vol.25 No.1
<P>Intratumoral hypoxia has been correlated with distant metastatic potential. Two hypoxia inducible factors (HIFs), HIF-1관 and HIF-2관, are induced by hypoxia, and high expression of these proteins has been correlated to angiogenesis and distant metastasis. Thymosin 관4 (T관4) is frequently highly expressed in cancer, and this overexpression correlates with malignant progression. The objective of this study was to investigate the clinical correlation of HIF-관 with T관4 and the intracellular functional roles of T관4 on HIF-관 activation. We examined HIF-1관, HIF-2관 and T관4 expressions in clinical human breast carcinoma (n=70) by immunohistochemistry. We show that high expression of HIF-1관 and HIF-2관 strongly correlates with T관4 expression (P??.0001) and overexpression of T관4 correlates significantly with patients with lymph node metastasis (P<0.05) of human breast cancer. Additionally, we demonstrate that hypoxia up-regulates intracellular T관4 protein, which then affects HIF-관 activity, which is the key in regulating VEGF expression. We confirmed that hypoxia-induced intracellular T관4 and HIF-관 activities were reduced by interference of T관4 expression using T관4 shRNA lentivirus. Vascular epidermal growth factor (VEGF)-A, a well-recognized lymphangiogenic cytokine, was also down-regulated, but VEGF-C and VEGF-D expressions were not affected. These findings suggest that the overexpression of T관4 is strongly associated with HIF-1관 and HIF-2관 expression and is also clinicopathologically involved with lymph node metastatic potential of breast cancer through the modulation of HIF-관activation and induction of VEGF-A. Ultimately, these results highlight T관4 as a potentially therapeutic target in malignant cancers.</P>
Yoon, Dong Suk,Lee, Kyoung-Mi,Kim, Sung-Hwan,Kim, Su Hee,Jung, Youngmee,Kim, Soo Hyun,Park, Kwang Hwan,Choi, Yoorim,Ryu, Hyun Aae,Choi, Woo Jin,Lee, Jin Woo Mary Ann Liebert 2016 Tissue engineering. Part A Vol.22 No.3
<P>The objective of this study was to determine whether a biphasic scaffold loaded with a combination of a chemokine and bone marrow concentrate (BMC) could improve tissue regeneration in knee articular cartilage of beagles with cylindrical osteochondral defects. For this investigation, an osteochondral defect (6mm in diameter and 8mm deep) was created in the weight-bearing articular surface of the femoral medial condyle in beagles. Bone marrow was aspirated from the posterior iliac crests of beagles to obtain mesenchymal stem cells (MSCs) for in vitro assay. Hematoxylin and eosin (HE), Masson's trichrome (MT), safranin O/fast green staining, and immunohistochemistry were performed for histological analysis. Quantitative real-time polymerase chain reaction was performed to understand the roles of BMC in chondrogenic differentiation of MSCs. At 12 weeks after transplantation of biphasic scaffolds, we observed that interleukin-8 (IL-8) or the combination of IL-8 and BMC induced massive bone regeneration compared to saline, BMC only, and MSCs. In gross appearance, the osteochondral defect site was nearly completely filled with repair tissue in the group that received the combination of IL-8 and BMC but not in the other groups. Moreover, histological analysis showed obvious differences in cartilage regeneration among groups. HE and MT staining showed that the cartilage defect sites of the group receiving the combination of IL-8 and BMC were regenerated with cartilage-like tissues showing chondrocyte morphology. Safranin O staining showed hyaline cartilage regeneration in the group receiving IL-8 and BMC, whereas fibrous-like tissues were formed in the other groups. Furthermore, immunostaining revealed the presence of type II collagen and aggrecan in regenerated cartilage tissue of the group receiving IL-8 and BMC, whereas regenerated cartilage tissues of the other groups weakly expressed type II collagen and aggrecan. These results indicate that the combination of a chemokine IL-8 and BMC has significant positive effects on osteochondral regeneration in a beagle model through enhancing expression of the chondrogenic transcription factors and markers such as Sox9 and type II collagen.</P>