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Yao, Xuerui,Jiang, Hao,NanXu, Yong,Piao, Xuanjing,Gao, Qingshan,Kim, Nam-Hyung Butterworths, etc 2019 Theriogenology Vol. No.
<P><B>Abstract</B></P> <P>Kaempferol (3,4′,5,7-tetrahydroxyflavone, KAE) is one of the most commonly occurring dietary flavonols. The biological and pharmacological effects of kaempferol depend upon whether it acts as an antioxidant, anti-inflammatory, or anticancer agent. The present study explored the influence of KAE supplementation on <I>in vitro</I> damage to porcine oocytes and its underlying mechanisms. Different concentrations of KAE (0.05, 0.1, 0.5, 1 μM) were added to porcine zygote medium 5 during <I>in vitro</I> culture. The results showed that supplementation with 0.1 μM KAE significantly increased the blastocyst formation rate. Blastocyst formation and quality were significantly increased in the 200 μM H<SUB>2</SUB>O<SUB>2</SUB> treatment group following addition of 0.1 μM KAE. KAE prevented the H<SUB>2</SUB>O<SUB>2</SUB>-induced compromise of mitochondrial membrane potential and reactive oxygen species generation. Furthermore, the extent of autophagy and DNA damage in the blastocysts was attenuated by supplementation with KAE in the H<SUB>2</SUB>O<SUB>2</SUB> induced oxidative injury group compared to that observed in controls. These results suggest that KAE has beneficial effects on the development of porcine parthenotes by attenuating oxidative stress and increasing mitochondrial function.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Kaempferol has beneficial effects on the development of porcine early stage embryos. </LI> <LI> Kaempferol prevented the H<SUB>2</SUB>O<SUB>2</SUB>-induced production of ROS. </LI> <LI> Kaempferol prevented mitochondrial dysfunction induced by H<SUB>2</SUB>O<SUB>2</SUB> in porcine embryos. </LI> <LI> Kaempferol prevented H<SUB>2</SUB>O<SUB>2</SUB>-induced autophagy and DNA damage. </LI> </UL> </P>
Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress
YAO, Xuerui,JIANG, Hao,LIANG, Shuang,SHEN, Xinghui,GAO, Qingshan,XU, Yong Nan,KIM, Nam-Hyung 家畜繁殖硏究所 2018 Journal of Reproduction and Development Vol.64 No.6
<P>Laminarin (LAM) is a β-glucan oligomer known to possess biological activities such as anticancer and antioxidant effects. This study explored the influence of LAM supplementation on <I>in vitro</I> aged porcine oocytes and the underlying mechanisms behind this influence. We found that LAM delayed the aging process and improved the quality of aged oocytes. LAM supplementation enhanced the subsequent developmental competence of aged oocytes during the <I>in vitro</I> aging process. The blastocyst formation rate was significantly increased in aged oocytes treated with 20 µg/ml LAM compared to non-treated aged oocytes (45.3% <I>vs</I>. 28.7%, P < 0.01). The mRNA levels of apoptosis-related genes, B cell lymphoma-2-associated X protein (<I>Bax)</I> and <I>Caspase-3,</I> were significantly lower in blastocysts derived from the LAM-treated aged oocytes during the <I>in vitro</I> aging process. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased in aged oocytes following LAM treatment. Mitochondrial membrane potential was increased, and the activities of caspase-3 and cathepsin B were significantly reduced in the LAM-treated aged oocytes compared with the non-treated aged oocytes. Taken together, these results suggest that LAM is beneficial for delaying the aging process in porcine oocytes.</P>
2i System Enhances Porcine Blastocyst Quality by Regulating of Epigenetic Modification
Jeongwoo Kwon,XingHui Shen,Jiang Hao,Yao Xuerui,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10
2i system is useful for maintenance of navie state of embryonic stem cells in various species. However, the role of 2i system in porcine preimplantation stage embryos unclear. In this study, we investigated the quality of blastocyst and pluripotent-related factor by 2i system. And, we also investigated about DNA and RNA epigenetic modification status by 2i system during porcine early embryo development. After treatment of MEK1/2 inhibitor PD0325901(4 μM), GSK-3 inhibitor CHIR99021(0.3 μM) and 2i (PD0325901+CHIR99021) during pre-implantation development, blastocyst formation rate had no significantly difference. However, blastocyst size increased in 2i treatment embryos. Blastocyst quality also increased after treatment of CHIR and 2i using TUNEL assay and Brdu assay. Interestingly, main transcription factor OCT4 and SOX2 positive cells in blastocyst was significantly increased in 2i groups. Especially, histone modification- relation proteins like h3k9me3 and h3k9ac changed after treatment of CHIR and 2i. Global gene expression patterns related with pluripotency and epigenetic also changed in inhibitor treatment groups. Furthermore, bi-sulfite sequencing results showed that 2i treatment groups demethylated in satellite I region. In conclusion, our findings strongly suggest that 2i system can be useful method for increasing of blastocyst quality by affecting of ICM/TE formation by regulating epigenetic modification during early embryo development.