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- 저자 : Yangyang Long (검색결과 2 건)
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Quantification of the Energy Flows during Ultrasonic Wire Bonding under Different Process Parameters
Yangyang Long,Friedrich Schneider,Chun Li,Jörg Hermsdorf,Jens Twiefel,Jörg Wallaschek 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.6 No.3
Despite of its wide and long-term application for interconnections in the field of microelectronics packaging, a quantitative understanding on the mechanisms of ultrasonic (US) wire bonding is still lacked. In this work, the energy flows from the electrical input energy to the different mechanisms during the US bonding process are quantified based on real-time observations via which the relative motions at the wire/substrate and the wire/tool interfaces can be detected. The relative motions at the two interfaces are proved to be caused by both the continuous plastic deformation and the US vibration. The normal force and US power interdependently affect the relative motion amplitudes. The deduced energy flows show that the energy from the transducer mainly flows to the vibration induced friction at the two interfaces and the microwelds formation, deformation and breakage. Despite of their significance to the process, the other mechanisms receive only little amount of energy. The impacts of the process parameters including normal force, US power and time on the energy flows are quantitatively investigated. A good coupling of the normal force and the US power guides more energy to the formation of microwelds while a long process time would increase the friction induced energy consumption.
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CDH17 nanobodies facilitate rapid imaging of gastric cancer and efficient delivery of immunotoxin
Jingbo Ma,Xiaolong Xu,Chunjin Fu,Peng Xia,Ming Tian,Liuhai Zheng,Kun Chen,Xiaolian Liu,Yilei Li,Le Yu,Qinchang Zhu,Yangyang Yu,Rongrong Fan,Haibo Jiang,Zhifen Li,Chuanbin Yang,Chengchao Xu,Ying Long,J 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4
Background: It is highly desirable to develop new therapeutic strategies for gastric cancer given the low survival rate despite improvement in the past decades. Cadherin 17 (CDH17) is a membrane protein highly expressed in cancers of digestive system. Nanobody represents a novel antibody format for cancer targeted imaging and drug delivery. Nanobody targeting CHD17 as an imaging probe and a delivery vehicle of toxin remains to be explored for its theragnostic potential in gastric cancer. Methods: Naïve nanobody phage library was screened against CDH17 Domain 1-3 and identified nanobodies were extensively characterized with various assays. Nanobodies labeled with imaging probe were tested in vitro and in vivo for gastric cancer detection. A CDH17 Nanobody fused with toxin PE38 was evaluated for gastric cancer inhibition in vitro and in vivo. Results: Two nanobodies (A1 and E8) against human CDH17 with high affinity and high specificity were successfully obtained. These nanobodies could specifically bind to CDH17 protein and CDH17-positive gastric cancer cells. E8 nanobody as a lead was extensively determined for tumor imaging and drug delivery. It could efficiently co-localize with CDH17-positive gastric cancer cells in zebrafish embryos and rapidly visualize the tumor mass in mice within 3 h when conjugated with imaging dyes. E8 nanobody fused with toxin PE38 showed excellent anti-tumor effect and remarkably improved the mice survival in cell-derived (CDX) and patient-derived xenograft (PDX) models. The immunotoxin also enhanced the anti-tumor effect of clinical drug 5-Fluorouracil. Conclusions: The study presents a novel imaging and drug delivery strategy by targeting CDH17. CDH17 nanobodybased immunotoxin is potentially a promising therapeutic modality for clinical translation against gastric cancer.
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