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        Cellulose Monoacetate/Tetraethyl Orthosilicate Hybrid Nanofibers for Electrochemical DNA Biosensors

        Sinem Civan,Seval Aydin,Nilay Aladag Tanik,Yakup Aykut 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4

        Cellulose monoacetate/tetraethyl orthosilicate (CMA/TEOS) hybrid nanofibers were produced with differentratios via electrospinning and used for guanine oxidation analysis in the single strand deoxyribonucleic acid (ssDNA)molecules by electrochemical method. Nanofiber (NF) diameters for pure CMA dramatically decreased from between 2.4 μ-306 nm to between 958-42 nm with addition of hydrochloric acid (HCl) catalyzer into the electrospinning solution. UniformCMA nanofibers morphologies transform to more defect structures containing particular structures with the addition andincrease of TEOS content in CMA electrospinning solution. Also, nanofibers' diameters range became more fluctuated formand ultrafine nanofibers (diameters below 100 nm) existed more in the nanofiber mat. Even though the melting point wasseen at CMA nanofibers, melting points were not detectable in CMA/TEOS NFs since TEOS addition does not allow anappropriate crystallization. Thermal analysis results revealed that residual contents after TGA measurement in nitrogenatmosphere were gradually increased by increasing TEOS ratio in CMA/TEOS nanofibers. ssDNA molecules were immobilizedon the as-spun nanofibers and differential pulse voltammetry (DPV) measurements were carried out to investigate theguanine oxidation in ssDNA. Guanine oxidation signal intensities decreased with the initial addition of TEOS to CMAnanofibers and increases again by increasing TEOS content in CMA/TEOS hybrid nanofibers and then gradually decreasesagain with increasing TEOS content. The prepared CMA/TEOS hybrid nanofibers could be a promising candidate as anelectrode interface for genetic molecule detection via electrochemical methods.

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