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( Chaohui Shen ),( Xiyang Gao ),( Tao Li ),( Jun Zhang ),( Yuqian Gao ),( Liyou Qiu ),( Guang Zhang ) 한국균학회 2019 Mycobiology Vol.47 No.3
The 11a-hydroxylation of 16a, 17-epoxyprogesterone (EP) catalyzed by Rhizopus nigricans is crucial for the steroid industry. However, lower conversion rate of the biohydroxylation restricts its potential industrial application. The 11a-steroid hydroxylase CYP509C12 from R. oryzae were reported to play a crucial role in the 11a-hydroxylation in recombinant fission yeast. In the present study, the CYP509C12 of R. oryzae (RoCYP) was introduced into R. nigricans using the liposome-mediated mycelial transformation. Heterologous expression of RoCYP resulted in increased fungal growth and improved intracellular reactive oxygen species content in R. nigricans. The H<sub>2</sub>O<sub>2</sub> levels in RoCYP transformants were approximately 2-folder that of the R. nigricans wild type (RnWT) strain, with the superoxide dismutase activities increased approximately 45% and catalase activities decreased approximately 68%. Furthermore, the 11a-hydroxylation rates of EP in RoCYP transformants (C4, C6 and C9) were 39.7%, 38.3% and 38.7%, which were 12.1%, 8.2% and 9.4% higher than the rate of the RnWT strain, respectively. This paper investigated the effect of heterologous expression of RoCYP in R. nigricans, providing an effective genetic method to construct the engineered strains for steroid industry.
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Shen, Chaohui,Gao, Xiyang,Li, Tao,Zhang, Jun,Gao, Yuqian,Qiu, Liyou,Zhang, Guang The Korean Society of Mycology 2019 Mycobiology Vol.47 No.3
The $11{\alpha}$-hydroxylation of $16{\alpha}$, 17-epoxyprogesterone (EP) catalyzed by Rhizopus nigricans is crucial for the steroid industry. However, lower conversion rate of the biohydroxylation restricts its potential industrial application. The $11{\alpha}$-steroid hydroxylase CYP509C12 from R. oryzae were reported to play a crucial role in the $11{\alpha}$-hydroxylation in recombinant fission yeast. In the present study, the CYP509C12 of R. oryzae (RoCYP) was introduced into R. nigricans using the liposome-mediated mycelial transformation. Heterologous expression of RoCYP resulted in increased fungal growth and improved intracellular reactive oxygen species content in R. nigricans. The $H_2O_2$ levels in RoCYP transformants were approximately 2-folder that of the R. nigricans wild type (RnWT) strain, with the superoxide dismutase activities increased approximately 45% and catalase activities decreased approximately 68%. Furthermore, the $11{\alpha}$-hydroxylation rates of EP in RoCYP transformants (C4, C6 and C9) were 39.7%, 38.3% and 38.7%, which were 12.1%, 8.2% and 9.4% higher than the rate of the RnWT strain, respectively. This paper investigated the effect of heterologous expression of RoCYP in R. nigricans, providing an effective genetic method to construct the engineered strains for steroid industry.
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Xue Li,Tingting Liu,Fei Tian,Xiyang Tao,Zhansheng Wu 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.11
The high cost and low carrier separation efficiency of g-C3N4/Ag2O photocatalysts affect its application in the degradation of organic pollutants. In this study, the CA-βCD/g-C3N4/Ag2O 2D/0D heterojunction photocatalysts were successfully prepared to enhance the visible light response and inhibit the electron-hole recombination simultaneously during pollutant degradation. The 10:1:1 CA-βCD/g-C3N4/Ag2O showed the outstanding photochemical catalysis performance for the degradation of organic pollutants. The degradation efficiency of methyl orange, reactive black and norfloxacin was 2.53, 1.92 and 1.14 times than that of 1:1 g-C3N4/Ag2O. In addition, 10:1:1 CA-β-CD/g-C3N4/Ag2O also showed excellent photocatalytic stability. The free radical scavenging experiment and electron spin resonance proved that ·O − 2 was the chief active specie in the degradation process. The mechanism research results showed that the formation of heterojunction improved the utilization rate of sunlight and promoted the separation efficiency of photo-generated electrons and holes, which significantly advanced the photocatalytic activity of the composite catalyst. The preparation of CA-βCD/g-C3N4/Ag2O provided ideas for modification of photocatalyst by macromolecular organic matter.
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