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RISS 인기검색어
- 검색키워드
- 저자 : Xinming Ji (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
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Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips
Zhang, Xuzhi,Ji, Xinming,Cui, Zhengguo,Yang, Bing,Huang, Jie Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.1
Five-channel electrochemical chips were fabricated based on the Micro-electromechanical System (MEMS) technology and were used as platforms to develop DNA arrays. Different kinds of thiolated DNA strands, whose sequences were related to white spot syndrome virus (WSSV) gene, were separately immobilized onto different working electrodes to fabricate a combinatorial biosensor system. As a result, different kinds of target DNA could be analyzed on one chip via a simultaneous recognition process using potassium ferricyanide as an indicator. To perform quantitative target DNA detection, a limit of 70 nM (S/N=3) was found in the presence of 600 nM coexisting noncomplementary ssDNA. The real samples of loop-mediated isothermal amplification (LAMP) products were detected by the proposed method with satisfactory result, suggesting that the multichannel chips had the potential for a high effective microdevice to recognize specific gene sequence for pointof-care applications.
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Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips
Xuzhi Zhang,Xinming Ji,Zhengguo Cui,Bing Yang,Jie Huang 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.1
Five-channel electrochemical chips were fabricated based on the Micro-electromechanical System (MEMS)technology and were used as platforms to develop DNA arrays. Different kinds of thiolated DNA strands,whose sequences were related to white spot syndrome virus (WSSV) gene, were separately immobilized onto different working electrodes to fabricate a combinatorial biosensor system. As a result, different kinds of target DNA could be analyzed on one chip via a simultaneous recognition process using potassium ferricyanide as an indicator. To perform quantitative target DNA detection, a limit of 70 nM (S/N=3) was found in the presence of 600 nM coexisting noncomplementary ssDNA. The real samples of loop-mediated isothermal amplification (LAMP) products were detected by the proposed method with satisfactory result, suggesting that the multichannel chips had the potential for a high effective microdevice to recognize specific gene sequence for pointof-care applications.
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