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- 저자 : Xiaomin Lian (검색결과 2 건)
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Xiaomin Liu,Chengbin Yang,Jing Liu,Jianwei Liu,Rui Hu,Hongwei Lian,Guimiao Lin,Liwei Liu,Ken-Tye Yong,Ling Ye 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
The optimization of aldose reductase (AR) expression levels and tracking of the AR expression sites within the cell is an essential step in developing a platform for the effective production of aldose reductase inhibitors (ARIs). In this study, we have demonstrated the use of both immunocytochemistry and quantum dots-based immunofluorescence techniques for observing and detecting the expression level and localization of AR in the cytoplasm and cell membrane of a eukaryotic cell model with high levels of AR protein expression. Our results show that high expression levels of human AR can be achieved using the eukaryotic cell model that we have developed. The overexpressed AR can be used for translational studies of hAR and the screening of ARIs. More importantly, the use of the established quantum dots-based immunofluorescence technique in the intracellular labeling of AR allows the determination of the expression and distribution of the AR gene. Overall, the use of the interdisciplinary approach of both genetic engineering and quantum dot-based immunofluorescence allows not only the effective production of a desired protein, but also the determination of the cellular localization of such an expressed protein.
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CONTROL OF DPPS IN VEHICLE USING FEED-FORWARD DECOUPLING
Hao Chen,Chao Wang,Xi Chen,Xiaomin Lian 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.4
To solve the contradiction between interior space and demand for electricity of vehicle, this paper brings forward the dual-generator parallel power system (DPPS). However, it is difficult to control the voltage and balance output power between two different types of generators simultaneously. Thus, feed-forward control has been developed to accomplish decoupling the target current of two generators, and proportional-integral differential (PID) controllers, with its stability being discussed, are also designed to track desired values. Based on structural analysis of DDPS, the simulation model is built in MATLAB/Simulink to prove the preliminary feasibility of the scheme and provide a reference for the real system. Finally, bench tests have been performed involving the dynamic change in the reference voltage, engine rotating speed, and load, to verify the effectiveness of the proposed method. The results show that it can achieve synchronous control of the output voltage and current ratio of two generators in DPPS.
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