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Buyue Niu,Dongchun Guo,Zhiran Liu,Xiaofei Han,Xibiao Wang 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.12
Objective: The cytokine inducible SH2-containing protein (CISH), which might play a role in porcine intestine immune responses, was one of the promising candidate genes for piglet anti-disease traits. An experiment was conducted to characterize the porcine CISH (pCISH) gene and to evaluate its genetic effects on pig anti-disease breeding. Methods: Both reverse transcription polymerase chain reaction (RT-PCR) and PCR were performed to obtain the sequence of pCISH gene. A pEGFP-C1-CISH vector was constructed and transfected into PK-15 cells to analysis the distribution of pCISH. The sequences of individuals were compared with each other to find the polymorphisms in pCISH gene. The association analysis was performed in Min pigs and Landrace pigs to evaluate the genetic effects on piglet diarrhea traits. Results: In the present research, the coding sequence and genomic sequence of pCISH gene was obtained. Porcine CISH was mainly localized in cytoplasm. TaqI and HaeIII PCR restriction fragment length polymorphism (RFLP) assays were established to detect single nucleotide polymorphisms (SNPs); A-1575G in promoter region and A2497C in Intron1, respectively. Association studies indicated that SNP A-1575G was significantly associated with diarrhea index of Min piglets (p<0.05) and SNP A2497C was significantly associated with the diarrhea trait of both Min pig and Landrace piglets (p<0.05). Conclusion: This study suggested that the pCISH gene might be a novel candidate gene for pig anti-disease traits, and further studies are needed to confirm the results of this preliminary research.
Study of thermoacoustic oscillations in half-open tubes for saturated superfluid helium
Xianjin Wang,Xiaofei Niu,Feng Bai,Junhui Zhang,Shuping Chen 한국초전도저온학회 2022 한국초전도저온공학회논문지 Vol.24 No.3
Thermoacoustic oscillations (TAOs) are spontaneous pressure oscillations frequently seen in hydrogen or helium cryogenicsystems. Half-open tubes connected to cryogenic fluid with a closed room temperature end have a high potential for oscillationgeneration. Thermoacoustic oscillations will result in significant pressure fluctuations and additional heat load, endangering thesecurity and stability of the cryogenic system. The goal of this paper is to investigate TAOs in superfluid helium using boththeoretical and experimental methods. Five half-open tubes with varied typical inner diameters inserted into superfluid helium wereinstalled in a test cryostat. The onset characteristics of thermoacoustic oscillations were presented and studied. The effect oftemperature profile was discussed. Finally, a simple eliminating method was introduced.