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- 저자 : Vino T. Cheriyan (검색결과 1 건)
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Sasidharan Nishanth Kumar,Bala Nambisan,B. S. Dileep Kumar,Nisha Girija Vasudevan,Chellapan Mohandas,Vino T. Cheriyan,Ruby John Anto 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.9
3,5-Dihydroxy-4-isopropylstilbene is a naturalphytoalexin and was first identified as bacterial secondarymetabolites. The aim of this study is to investigate in vitroantioxidant and anticancer activity of 3,5-dihydroxy-4-isopropystilbene purified from the cell free culture filtrateof Bacillus sp. N strain associated with rhabditid entomopathogenicnematode. Antioxidant activity was evaluatedby five separate methods: free radical scavenging, reducingpower assay, chelating effects on ferrous ions, NBTsuperoxide radical scavenging assay and hydroxyl radicalscavenging activity. The stilbene recorded powerful antioxidantactivity at various antioxidant systems in vitro. Thesuperoxide radical scavenging (92.1 %) and hydroxylradical scavenging (83.4 %) activities of the stilbenes at100 lg/ml were higher than the butylated hydroxyanisole,the known antioxidant agent. Anticancer activity of stilbenewas tested against breast cancer (MDAM B-231),cervical cancer (HeLa), lung cancer (A 549), colon cancer (HTL 116) cell lines using MTT method. The induction ofapoptosis was studied by morphological analysis, apoptoticcell staining, caspase 3 activation assay and cell cycleanalysis using flow cytometry. Stilbene induced significantmorphological changes and DNA fragmentation associatedwith apoptosis in HeLa cells. Acridine orange/ethidiumbromide stained cells indicated apoptosis induction bystilbene. Up-regulation of caspase 3 activity was also foundin cells treated with stilbene. Flow cytometry analysisshowed an increase in the percentage of apoptotic cells insub G0 phase (2.4 % in control plates to 11.4 %in 25 lg/mlof stilbene) confirming the stilbene induced apoptosis. Theresults of the present study showed that stilbene demonstrateda strong antioxidant and anticancer effects. Thesesuggest that stilbene may be used as possible naturalantioxidant and anticancer agents to control various humandiseases.
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