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Mehdi Salehi,Abdollah Hatamzadeh,Vahab Jafarian,Shahin Zarre 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.4
The antioxidative potential and some phytochemicals of Lilium ledebourii Boiss. were biochemically analyzed using assays of extracts from the bulb, shoot and fl ower. In addition, the plant’s biosystematics was molecularly characterized using a phylogenetic tree. L. ledebourii plants were collected from their natural habitat. Biochemical assays showed a higher total phenolic and total fl avonoid content in the leaf than in the bulb (by 264.9% and 27.38%, respectively) and fl ower (by 331.18% and 47.85%, respectively). The fl ower had the highest total anthocyanin of all the organs. The DPPH-based antioxidant activity assay showed the highest activity level in the bulb, whereas FRAP- and BCLS-based assays revealed higher antioxidant activity in the leaf than in the bulb (by 7.1 and 3 folds, respectively) and fl ower (by 4.9 and 1.5 folds, respectively). Our results showed that phenolic and fl avonoid compositions were positively associated with antioxidative potential as assayed by FRAP and BCLS methods. The 5.8S ribosomal DNA sequence was extracted by PCR from genomic DNA and sequenced. Our molecular study resulted in a sequencing record of L. ledebourii var. Kelardasht Salehi 1 in Genbank with the accession number KX495217.1. We conclude that the antioxidant potential of L. ledebourii is strongly dependent on the organ and assay method.
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