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        Evaluation of Aflatoxin B1 Biosynthesis in A. flavus Isolates from Central India and Identification of Atoxigenic Isolates

        Tarun Kumar Patel,Rajesh Anand,Agam Prasad Singh,Jata Shankar,Bhupendra N. Tiwary 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6

        Present study describes the effect of temperatureand pH on biosynthesis of aflatoxin B1 (AFB1) to identifyatoxigenic (aflatoxin non-producer) isolates of Aspergillusflavus. An indigenous detection method for AFB1 wasdeveloped using tandem mass spectrometric method. Detection of AFB1 was carried out in positive polarityusing triple quadrapole mass spectrometer with electrosprayionization interface in Multiple Reaction Monitoring(MRM) mode. A total of four A. flavus isolates wereselected for screening of AFB1 biosynthesis at pH 4.0, 5.5,7.0, and 8.5 at 27°C. Highest AFB1 biosynthesis inMTCC11866 was found at pH 6.5 while in MTCC9367 itwas at pH 7.0. On screening AFB1 biosynthesis in A. flavusculture at various temperatures a significant suppression inAFB1 biosynthesis was found at 37°C in comparison to 24and 27°C in MTCC11866 and MTCC9367. AFB1 was notdetected in other two A. flavus isolates MTCC11580 andMTCC11588 at any temperature and pH tested. AFB1nonproducing isolates, MTCC11580 and MTCC11588could be used as potent biocontrol agent. Additionally,present standardized method for AFB1 detection may findits application in qualitative and quantitative analysis ofAFB1 contamination in food crop and other products.

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        A practical approach for kinetic analysis of hydrogenation of complex mineral base oil

        Modi Siddharth,Tiwari Anand Kumar,Rao Meka Srinivasa,Snigdha Thummalapalli,Saritha Thummalapalli,Gupta Thummalapalli Chandra Sekhara Manik,Kumar Ajay 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.7

        The mineral base oil contains paraffins, naphthenes and aromatic hydrocarbons (AH) with carbon chains ranging from C14 to C60. The presence of AH in base oil affects the performance of the product in many industrially oriented applications. The base oil considered in this work had AH around 14% w/w that needed to be reduced below 5% w/w for some applications and ideally 0% w/w. This paper demonstrates the practical approach for hydrogenation of complex mineral base oil for reducing AH. The mineral base oil rich in C20 was taken as the representative component. The hydrogen solubility in the oil was estimated using NRTL model. The semi-batch hydrogenation experiments were performed at different conditions and conversion of AH as high as 79% (i.e. 3% w/w) could be achieved. A second-order pseudo-homogeneous reaction kinetic model was proposed and validated. The conditions for reaction kinetics were optimized to achieve desirable conversion using Aspen Plus. To develop a continuous process for hydrogenation of AH, experiments were performed in a lab scale fixed bed reactor and the applicability of the kinetic model was validated. The kinetics was observed to be free of internal and external mass transfer limitations under lab scale conditions.

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