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Inhibition Effect of Flavonoid Compounds against Neuraminidase Expressed in Pichia pastoris
Nguyen, Thi Thanh Hanh,Kang, Hee-Kyoung,Kim, Young-Min,Jang, Tae-Su,Kim, Doman 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1
Neuraminidase (NA) is one of the two glycoproteins on the surface of influenza virus, which cleaves terminal sialic acid residues and facilitates the release of virions from infected cells. The recombinant NA from H5N1 influenza virus strain A/Vietnam/1203/04 was expressed in Pichia pastoris X33 as a 45 kDa protein that displayed a $K_m$ of $9.96{\pm}1.26{\mu}M$ with fluorogenic substrate, 2'-(4-methylumbelliferyl)-${\alpha}$-D-N-acetyl neuraminic acid. Partially purified NA was used for the inhibition and kinetic assays with eight flavonoid compounds and gallic acid. Among them, gallocatechin gallate (GCG) showed the best inhibition against NA with the $IC_{50}$ of $8.98{\pm}0.46{\mu}M$ and showed a competitive inhibition pattern with Ki value of $8.34{\pm}0.25{\mu}M$. In molecular docking experiments, GCG displayed a binding energy of -13.71 kcal/mol to the active site of NA and the galloyl moiety was required for NA inhibition activity.
An Empirial Study on Vietnam’s Trade Faclilitation in the Digital Economy
Thanh Huong Vu(Thanh Huong Vu ),Thi Thuy Hanh Lam(Thi Thuy Hanh Lam ),Ha Phuong Nguyen(Ha Phuong Nguyen ) The International Academy of Global Business and T 2023 The International Academy of Global Business and T Vol.19 No.2
Purpose - Vietnam is among the fastest growing digital economies in the Southeast Asian region, and has made enormous efforts to adopt digital measures to facilitate trade. The paper aims to assess Vietnam’s performance of digital trade facilitation and estimate the impact of digital trade facilitation on Vietnam’s exports to the Association of Southeast Asian Nations (ASEAN). Design/Methodology/Approach - Vietnam’s implementation of digital trade facilitation is assessed through a constructed framework based on two groups of indicators, including Information Availability and Formalities Automation extracted from the OECD Trade Facilitation database. Further, a gravity model was adopted to estimate the impact of digital trade facilitation on Vietnam’s exports. Findings - The results show that Vietnam performs a slightly better than the average level of ASEAN countries. However, Vietnam’s implementation of digital trade facilitation tends to be ỉmproving at a slower rate than other ASEAN countries’ implementations, as well as the country’s overall implementation of other trade facilitation. The paper is among leading studies to quantify the impact of digital trade facilitation on Vietnam’s exports to ASEAN countries, and finds that if Vietnam and ASEAN countries increase trade facilitation through Information Availability measures by 1%, Vietnam’s exports will increase more than proportionately by 1.29% and 1.01%, respectively. Meanwhile, the digitalization of trade formalities so far has had no effect on Vietnam’s exports to this region. Research Implications - Vietnam should place priority on enhancing automation in trade-related administrative procedures, especially in customs procedures. Furthermore, it should pay more attention to promote the electronic publication of trade-related information, enhance the transparency of government policymaking, and increase the quality of enquiry points.
Flavonoid-mediated inhibition of SARS coronavirus 3C-like protease expressed in Pichia pastoris.
Nguyen, Thi Thanh Hanh,Woo, Hye-Jin,Kang, Hee-Kyoung,Nguyen, Van Dao,Kim, Young-Min,Kim, Do-Won,Ahn, Sul-Ah,Xia, Yongmei,Kim, Doman Kluwer Academic Publishers 2012 Biotechnology letters. Vol.34 No.5
<P>The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome associated coronavirus (SARS-CoV) is vital for SARS-CoV replication and is a promising drug target. Recombinant 3CL(pro) was expressed in Pichia pastoris GS115 as a 42??kDa protein that displayed a K ( m ) of 15??±??2??관M with Dabcyl-KTSAVLQSGFRKME-Edans as substrate. Purified 3CL(pro) was used for inhibition and kinetic assays with seven flavonoid compounds. The IC(50) of six flavonoid compounds were 47-381??관M. Quercetin, epigallocatechin gallate and gallocatechin gallate (GCG) displayed good inhibition toward 3CL(pro) with IC(50) values of 73, 73 and 47??관M, respectively. GCG showed a competitive inhibition pattern with K ( i ) value of 25??±??1.7??관M. In molecular docking experiments, GCG displayed a binding energy of -14??kcal??mol(-1) to the active site of 3CL(pro) and the galloyl moiety at 3-OH position was required for 3CL(pro) inhibition activity.</P>
Nguyen, Thi Thanh Hanh,Jung, Sun-Hwa,Lee, Sun,Ryu, Hwa-Ja,Kang, Hee-Kyoung,Moon, Young-Hwan,Kim, Young-Min,Kimura, Atsuo,Kim, Doman 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.5
Human intestinal maltase (HMA) is an ${\alpha}$-glucosidase responsible for the hydrolysis of ${\alpha}$-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA has become an important target in the treatment of type-2 diabetes. In this study, epigallocatechin gallate (EGCG) and EGCG glucoside (EGCG-G1) were identified as inhibitors of HMA by an in vitro assay with $IC_{50}$ of $20{\pm}1.0$ and $31.5{\pm}1.0{\mu}M$, respectively. A Lineweaver-Burk plot confirmed that EGCG and EGCG-G1 were competitive inhibitors of maltose substrate against HMA and inhibition kinetic constants ($K_i$) calculated from a Dixon plot were $5.93{\pm}0.26$ and $7.88{\pm}0.57{\mu}M$, respectively. Both EGCG and EGCG-G1 bound to the active site of HMA with numerous hydrophobic and hydrogen bond interactions.
Characterization and Biochemical properties of water soluble quercetin in steviol glucosides
Thi Thanh Hanh NGUYEN,ChangSeop SEO,Namhyeon PARK,Jungmin HA,DongGu LEE,Songhee HAN,Tae-kyung LEE,Jaewon HUR,Chaeri SONG,Shina JIN,So-hyung KWAK,Jeesoo KIM,Jinbeom SI,Doman KIM 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.10