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플라즈마 표면 처리 된 PDMS 막의 인장으로 생성된 미세패턴을 이용한 세포 접촉 유도 연구
유사무엘(Samuel Yu),최성균(Seongkyun Choi),요시츠미 마스모토(Yoshizumi Masumoto),타이치 히로세(Taichi Hirose),마사히토 반(Masahito Ban),김선민(Sun Min Kim) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10
Recently, contact guidance of cells using micro/nanopatterns has been widely investigated for mimicking in vivo environments. In this study, we used different types of micro-patterned surface as contact guidance for enhancing cell adhesion and growth. Micro/nanopatterns were created by stretching PDMS membrane exposed to plasma. Three different types of cells were cultured for 3 days on patterned PDMS surface to analyze cell growth and proliferation with phase contrast microscope. Also, the distribution of angle between patterns axis and cells axis were measured. Cultured cells on patterned PDMS surface were elongated along contact guidance and morphology of them was changed according to size of pattern and type of cell. This study will be helpful for the study on cell-surface interactions.