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Micropropagation of Aristolochia elegans(Mast.)
Lidia, Osuna T.,Alejandro, Mora I.,Elsa, Ventura Z.,Enrique, Jimenez F.,Crescencio, Bazaldua M.,Antonio, Jimenez A. The Korean Society of Crop Science 2007 Journal of crop science and biotechnology Vol.10 No.3
The roots of Aristolochia elegans Mast.(Aristolochiaceae) are widely used in Mexican traditional medicine as a remedy for scorpion venom. Current experimental evidence supports its purported antidote properties. However, collection from the wilderness has lead to local extinction of natural populations. In order to contribute to species preservation, cultivation, and standardization of morphological and pharmacological properties, a micropropagation method was developed. This includes in-vitro germination of seeds to produce aseptic plantlets, induction of multiple budding, and acclimatization. The treatment with benzylamino purine(10 ${\mu}M$) induced the highest number of buds(3.1 on average) in both types of explants. On the other hand, indolebutyric acid(1.5 ${\mu}M$) caused the highest root index(11.8) per explant. One hundred percent of the micropropagated plantlets developed vigorously after the acclimatization process.
Tasior, Mariusz,Hassanein, Khaled,Mazur, Leszek M.,Sakellari, Ioanna,Gray, David,Farsari, Maria,Samoć,, Marek,Santoro, Fabrizio,Ventura, Barbara,Gryko, Daniel T. The Royal Society of Chemistry 2018 Physical chemistry chemical physics Vol.20 No.34
<P>A three-step synthetic route to a structurally unique π-expanded pyrrolo[3,2-<I>b</I>]pyrrole derived bis-ketone has been developed. In contrast to all previous ladder-type pyrrolopyrroles, the new dye exhibits a low-energy absorption band in the visible region which is responsible for its red-purple color. Interestingly, even though the compound is centrosymmetric, this band coincides with the lowest energy two-photon absorption (TPA) transition. This non-typical behaviour has been computationally rationalized by finding two close lying excited states, one of which (S1) is active for OPA and the other (S2) for TPA processes, which arise from the mixing of two symmetric partial charge-transfer states. The ultrafast excited-state dynamics was characterized by means of transient absorption analysis. A relaxation process involving S1 symmetry breaking occurs in a few ps, leading to the formation of the lowest energy charge-transfer state. This is weakly emitting, with a measured lifetime in the order of tens of picoseconds. Interestingly, two-photon polymerization has been achieved using this new ketone. The high yield of radical photo-initiation upon two-photon excitation was demonstrated by the fabrication of woodpile photonic crystal templates by direct laser writing using a zirconium-silicon hybrid composite.</P>