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Hyun-Soo Kim,In-WookHwang,Su-MiKim,Chul-JoongKim,Kwang-SoonShin,Hyun-SooKim 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.1
The ORF5 gene ncodes a major envelopeglycoprotein (GP5), which is one of the thre majorproteins of porcine reproductive and respiratorysyndrome virus (PRRSV). The GP5 protein has benknown to be a 24.5-26kDa N-glycosylated envelopeprotein. The GP5 is involved in inducing neutralizingantibodies. For this reason, the GP5 is primary can-didate for the PRRSV subunit vaccine. To produce thecloned t h e O R F 5 g e n e f r o m P R S V C N V - 1 i n t o t h eSemliki Forest virus (SFV)-based expresion vector,resulting in recombinant pSFV-ORF5. By the infectionwith recombinant pSFV-ORF5 to BHK-21 cells, theGP5 expresion was confirmed by imunocytochemistryand imunobloting asay. The recombinant virusparticle harboring ORF5 gene was infectious to BHK-21and MARC-145. The RNA synthesis and expression ofGP5 in the infected cell was also confirmed byRT-PCR.