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Santos, Inê,s C.,Smuts, Jonathan,Choi, Woo-Sik,Kim, Younghoon,Kim, Seoung Bum,Schug, Kevin A. Elsevier 2018 Talanta Vol.182 No.-
<P><B>Abstract</B></P> <P>The identification of microorganisms is very important in different fields and alternative methods are necessary for a rapid and simple identification. The use of fatty acids for bacterial identification is gaining attention as phenotypic characteristics are reflective of the genotype and are more easily analyzed. In this work, gas chromatography-vacuum ultraviolet spectroscopy (GC-VUV) was used to determine bacteria fatty acid methyl esters (FAMEs), to identify and discriminate different environmental bacteria based on their fatty acid profile. Microorganisms were grown in agar and their fatty acids extracted, saponified, and esterified before analysis. Unique FAME profiles were obtained for each microorganism mainly composed of branched, cyclopropane, hydroxy, saturated, and unsaturated fatty acid methyl esters. <I>S. maltophilia</I> showed a higher diversity of fatty acids while <I>Bacillus</I> species showed higher complexity in terms of branched-chain FAMEs, with several <I>iso</I> and <I>anteiso</I> forms. 12 different bacteria genera and 15 species were successfully differentiated based on their fatty acid profiles after performing PCA and cluster analysis. Some difficult to differentiate species, such as <I>Bacillus</I> sp., which are genetically very similar, were differentiated with the developed method.</P> <P><B>Highlights</B></P> <P> <UL> <LI> GC-VUV of FAMEs can effectively differentiate a wide range of environmental bacteria. </LI> <LI> A fast method for FAMEs is featured; chromatographic resolution can be sacrificed for spectral resolution. </LI> <LI> Multivariate statistical methods indicate high classification accuracy. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>