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        Analysis of influencing parameters and reactive substance for enrofloxacin degradation in a dielectric barrier discharge plasma/peroxydisulfate system

        Shilin Song,Huijuan Wang,Yuyue Huang,Yixing Ma 대한환경공학회 2024 Environmental Engineering Research Vol.29 No.4

        In this paper, the chemical and physical effects generated during the dielectric barrier discharge plasma (DBDP) process, including O₃, H₂O₂, light, e<SUP>*</SUP>, etc., were utilized to activate the peroxydisulfate (PDS) to form SO₄<SUP>−</SUP>•. Then, the original reactive oxygen species (ROS), such as •OH, formed in the discharge system was combined to degrade the enrofloxacin (ENR) in water, and the corresponding influencing parameters and reactive substance in the DBDP/PDS system were analyzed. From the investigation, it was found that the neutral (pH =6.5) solution was more conducive to the ENR decomposition than the acidic and alkaline solution conditions. The presence of Fe<SUP>2+</SUP> and Cu<SUP>2+</SUP> in the reaction solution could hasten the ENR degradation, whereas the addition of Cl<SUP>−</SUP> and the HCO₃<SUP>−</SUP> in the solution had a negative effect. Analysis of the reactive species and quenching tests were carried out to explore the generation of H₂O₂, O₃, •OH, and SO₄<SUP>−</SUP>• in the DBDP/PDS system and their effects on the ENR degradation. The UV-Vis and 3D fluorescence spectra analysis were applied to demonstrate the cooperative effects of the DBDP and the PDS. The TOC and COD removals of the ENR solutions in the DBDP and the DBDP/PDS systems were also compared. Based on the intermediates analysis of the ENR degradation, three possible pathways of ENR decomposition in the synergistic system have been inferred.

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        The growth improvement of DvGS2-transgenic Arabidopsis thaliana arises from the higher efficiency of nitrogen and carbon assimilation

        Chenguang Zhu,Shilin Chen,Guimin Zhang,Wei Wang,Yuanping Tang,Bing Mei,Rentao Song 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.4

        The overexpression of algal glutamine synthetase (GS) gene DvGS2 in Arabidopsis thaliana resulted in higher plant biomass and better growth phenotype. The purpose of this study was to understand the biological mechanism for the improvement of growth phenotype of DvGS2-transgenic Arabidopsis. A series of molecular and biochemical investigation related to nitrogen and carbon metabolism in DvGS2-transgenic line was, respectively, conducted. Analysis of nitrogen use efficiency (NUE)-related gene expression and enzymatic activity revealed that the transcriptional level and enzymatic activity of genes encoding glutamate synthase, glutamate dehydrogenase and alanine aminotransferase were significantly up-regulated especially under low nitrate condition. The DvGS2- transgenic line showed higher total nitrogen content and lower carbon: nitrogen ratio than the wild type. Significant reduced concentration of leaf free ammonium, higher concentrations of total amino acids, individual amino acid (glutamine, methionine, alanine, asparagine) together with increased concentrations of glucose, fructose and starch, confirmed the higher efficiency of nitrogen and carbon assimilation. These improved metabolisms of nitrogen and carbon conferred the DvGS2-transgenic Arabidopsis more biomass, better growth phenotype and higher NUE compared with the wild type plant.

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