http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Shamayra S. SMAIL (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
U4 snRNA variants of Bombyx mori
Shamayra S. SMAIL,Rene J. HERRERA 한국곤충학회 2007 Entomological Research Vol.37 No.4
It is known that the spliceosome’'s catalytic core is composed of U2, U6 and U5 small nuclear (sn)RNAs. U6 is chaperoned and held inactive by intermolecular base-pair interactions by the U4 snRNA molecule until its incorporation into the spliceosomal complex. In previous studies, a number of variant forms of U1, U2 and U6 were detected in the silk moth Bombyx mori. Considering U4’'s unique role as a modulator of U6’'s activity, the aim of the present study was to determine if the multiplicity of U6 variants observed in B. mori is complemented in U4. Five U4 variants were identified in the B. moriWhole Genome Shotgun (WGS) database and were used to design internal U4 snRNA primers. These oligonucleotides were subsequently used to create four U4 reverse transcription (RT)-polymerase chain reaction (PCR) libraries with RNA extracted from the silk gland of B. mori. Two major U4 variants were identified from these libraries. The majority of the variation among genomic and transcript-derived U4 variant sequences is located in stem-loop II and III as well as at the 3′-end. No differences between the isoforms are located in the critical U4/U6 intermolecular binding regions. Sequence comparison analyses based on U4 intraspecific and interspecific variability delineate several evolutionarily conserved regions.
-
U2 snRNA variants are differentially incorporated into spliceosomes
Jason A. SOMARELLI,Annia MESA,Shamayra S. SMAIL,Angel L. ARES,Rene J. HERRERA 한국곤충학회 2009 Entomological Research Vol.39 No.2
In this study, five U2 small nuclear (sn)RNA variants were detected in the posterior silk gland of the Bombyx mori Nistari strain, one of which represents a novel U2 isoform not previously identified in other strains of this species. Following glycerol gradient ultracentrifugation of B. mori silk gland whole cell lysate, the newly isolated variant, U2α, was detected at a greater frequency in total cell lysate than in a high density glycerol gradient fraction rich in spliceosomal complexes. Conversely, previously identified isoforms U2A, U2B, U2D and U2N are abundant in the fraction containing high molecular weight spliceosomal complexes, possibly indicating their greater involvement in splicing. As expected, western blot and semi-quantitative reverse transcription-polymerase chain reaction experiments indicate high levels of specific serine and arginine rich (SR) proteins and total U2 snRNA (all variants included) in the fraction enriched in spliceosomes. Free energy values for each U2 isoform, as well as their individual stem-loops, were estimated to determine their structural stability. Due to the essential role of U2 in the transesterification reactions, it is possible that these isoforms may modulate splicing through differential incorporation into the spliceosome.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
