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- 저자 : Shahid Hameed (검색결과 7 건)
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Aish Muhammad,Cho Gyoungrae,Iqbal Hussain,Kazim Ali,Muhammad Zeeshan,Saima Noor,Hina Hafeez,Shahid Hameed,Shahid Hameed,Cha Jaebeom,Waqas Ahmed Dogar 한국국제농업개발학회 2022 한국국제농업개발학회 학술대회 Vol.2022 No.09
Potato is an important cash crop of Pakistan and widely cultivated in plains and mountains of Punjab. Khyber Pakhtun Khawa Gilgit Baltistan, on an area of 0.313 million hectares in summer, autumn and spring with an annual production of 07.9 million tons. In Pakistan potato yield is 25.2 tons per hectare, which is although above the world average but comparatively low as compare to the potential and other advance potato growing countries because of several reasons. Availability of healthy seeds and advance production technology are the major constraints to achieve the higher potato production goals. It is very alarming that in spite of good position in potato growing countries, we are unable to produce good quality seed. In Pakistan >01% quality seed is available against the seed demand around 0.5-0.7 million tons per annum and mostly depend upon the imported seed 15000-20000 tons per annum. Tissue culture industry has been a proven global technology in potato seed production, so it is dire need of the growing population and climatic change to adopt these modern technologies of potato production for sustainable and higher production and income to contribute potato growing farmers of Pakistan. It is possible with the commercial use of tissue culture and allied techniques as aeroponic potato seed production along with good management and plant protection measures to provide indigenous high vigor and high yield potential potato seed. Pakistan Agricultural Research Council has established Aeroponic Potato Seed Production facility at National Agricultural Research Center with the collaboration of Korean Govt RDA-KOPIA Pakistan. The Aeroponics is a modern technology for growing plants by providing a nutrient solution in the air without soil. Plants grow rapidly in the growth chamber under the aseptic environment, balanced nutrition and sufficient oxygen availability that improves potatoes production in the aeroponic system. This can produce 10 times more yield than conventional production systems. Aeroponics techniques are credited for making potato yield more efficient, and can reduce the number of steps in the potato seed multiplication. Through tissue culture and aeroponics facilities development in Pakistan, first ever more than 200,000 nucleus mini harvested at NARC from the autumn 2021 and spring 2022 planation at KOPIA screen houses. Further seed multiplication from the harvest is under progress at up hills and more than 500,000 tubers (G1) are expected to be harvested during October-November 2022. It is very encouraging, impressive and successful venture of KOPIA at Pakistan for the potato seed production and self-sufficiency to insure food security in the country.
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Hameed, Amir,Iqbal, Zafar,Asad, Shaheen,Mansoor, Shahid The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.4
Viral diseases have been a major limiting factor threating sustainable potato (Solanum tuberosum L.) production in Pakistan. Surveys were conducted to serologically quantify the incidence of RNA viruses infecting potato; Potato virus X (PVX), Potato virus Y (PVY), Potato virus S (PVS), Potato virus A (PVA), Potato virus M (PVM) and Potato leaf roll virus (PLRV) in two major potato cultivars (Desiree and Cardinal). The results suggest the prevalence of multiple viruses in all surveyed areas with PVY, PVS and PVX dominantly widespread with infection levels of up to 50% in some regions. Co-infections were detected with the highest incidence (15.5%) for PVX and PVS. Additionally the data showed a positive correlation between co-infecting viruses with significant increase in absorbance value (virus titre) for at least one of the virus in an infected plant and suggested a synergistic interaction. To test this hypothesis, glasshouse grown potato plants were challenged with multiple viruses and analyzed for systemic infections and symptomology studies. The results obtained conclude that multiple viral infections dramatically increase disease epidemics as compared to single infection and an effective resistance strategy in targeting multiple RNA viruses is required to save potato crop.
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Amir Hameed,Zafar Iqbal,Shaheen Asad,Shahid Mansoor 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.4
Viral diseases have been a major limiting factor threatingsustainable potato (Solanum tuberosum L.) productionin Pakistan. Surveys were conducted to serologicallyquantify the incidence of RNA viruses infectingpotato; Potato virus X (PVX), Potato virus Y (PVY),Potato virus S (PVS), Potato virus A (PVA), Potato virusM (PVM) and Potato leaf roll virus (PLRV) in twomajor potato cultivars (Desiree and Cardinal). Theresults suggest the prevalence of multiple viruses in allsurveyed areas with PVY, PVS and PVX dominantlywidespread with infection levels of up to 50% in someregions. Co-infections were detected with the highestincidence (15.5%) for PVX and PVS. Additionally thedata showed a positive correlation between co-infectingviruses with significant increase in absorbance value(virus titre) for at least one of the virus in an infectedplant and suggested a synergistic interaction. To testthis hypothesis, glasshouse grown potato plants werechallenged with multiple viruses and analyzed for systemicinfections and symptomology studies. The resultsobtained conclude that multiple viral infections dramaticallyincrease disease epidemics as compared tosingle infection and an effective resistance strategy intargeting multiple RNA viruses is required to save po-tato crop.
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Amir Hameed,Rakhshanda Bilal,Farooq Latif,Joyce Van Eck,Georg Jander,Shahid Mansoor 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.3
Potato tubers must be cold-stored to extend their shelf life and maintain an uninterrupted supply chain for food processors. However, a side-effect of low-temperature storage is manifested in terms of cold-induced sweetening (CIS) of potato tubers, which reduces the processing quality and the commercial value of the end-products. RNA interference (RNAi) technology, whereby transgene-derived small interfering RNAs can trigger the homology-based knockdown of cognate host genes and can initiate gene silencing, has been successfully applied in crop improvement through targeted gene knockout in host plants. In the current study, transgenic potato plants (Solanum tuberosum cv. Désirée) were generated, expressing a 300 bp hairpin loop nucleotide sequence targeting the potato vacuolar invertase gene (VInv), under the constitutive Cauliflower mosaic virus 35S promoter. Tubers collected from transgenic lines showed a significant reduction in reducing sugar content after 180 days of cold storage, without showing any measurable off-target effects on plant morphology and tuberization compared to nontransformed control plants. The cold-stored tubers were further assayed for chip color, which showed a fairly light colored quality in the samples originating from RNAi lines. Together with similar effects seen in previously published experiments involving other potato varieties, the Désirée results described here establish the efficacy of using RNAi for the successful reduction of CIS in potato tubers.
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Muhammad Nouman Tahir,Amir Hameed,Imran Amin,Shahid Mansoor 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.5
Spinach is a vegetable crop which is widely grown over a large area especially in Punjab province of Pakistan. Leaf curling and enations on spinach plant collected shown to be associated with the begomovirus Pedilanthus leaf curl virus (PeLCV) and Shahdadpur strain of Cotton leaf curl Multan betasatellite (CLCuMBSha). Defective molecules of half and quarter size derived from monopartite begomoviruses are usually generated by the deletion of virion-sense sequences. Characterization of defective molecules of PeLCV from spinach revealed that the molecules of half the size are derived from the deletion of complementary-sense genes while quarter size molecule appears to have evolved by further deletion. This is the first report of the begomovirusbetasatellite complex on spinach and unusual defective molecules derived from deletion of complementarysense genes in Pakistan.
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Tahir, Muhammad Nouman,Hameed, Amir,Amin, Imran,Mansoor, Shahid The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.5
Spinach is a vegetable crop which is widely grown over a large area especially in Punjab province of Pakistan. Leaf curling and enations on spinach plant collected shown to be associated with the begomovirus Pedilanthus leaf curl virus (PeLCV) and Shahdadpur strain of Cotton leaf curl Multan betasatellite ($CLCuMB^{Sha}$). Defective molecules of half and quarter size derived from monopartite begomoviruses are usually generated by the deletion of virion-sense sequences. Characterization of defective molecules of PeLCV from spinach revealed that the molecules of half the size are derived from the deletion of complementary-sense genes while quarter size molecule appears to have evolved by further deletion. This is the first report of the begomovirus-betasatellite complex on spinach and unusual defective molecules derived from deletion of complementary-sense genes in Pakistan.
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