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Whole-genome sequence analysis of Zika virus, amplified from urine of traveler from the Philippines
Gu, Se Hun,Song, Dong Hyun,Lee, Daesang,Jang, Jeyoun,Kim, Min Young,Jung, Jaehun,Woo, Koung In,Kim, Mirang,Seog, Woong,Oh, Hong Sang,Choi, Byung Seop,Ahn, Jong-Seong,Park, Quehn,Jeong, Seong Tae Springer US 2017 Virus genes Vol.53 No.6
<P>Zika virus (ZIKV) (genus <I>Flavivirus</I>, family <I>Flaviviridae</I>) is an emerging pathogen associated with microcephaly and Guillain-Barré syndrome. The rapid spread of ZIKV disease in over 60 countries and the large numbers of travel-associated cases have caused worldwide concern. Thus, intensified surveillance of cases among immigrants and tourists from ZIKV-endemic areas is important for disease control and prevention. In this study, using Next Generation Sequencing, we reported the first whole-genome sequence of ZIKV strain AFMC-U, amplified from the urine of a traveler returning to Korea from the Philippines. Phylogenetic analysis showed geographic-specific clustering. Our results underscore the importance of examining urine in the diagnosis of ZIKV infection.</P>
시중 판매되는 발효소시지의 이화학적 및 관능적 특성 연구
사공헌구(Hun Gu Sagong),강기문(Ki Moon Kang),박종세(Jong Se Park),정동순(Dong Soon Jeong),문병석(Byoung Seok Moon),황한준(Han Joon Hwang),유현희(Hyun Hee Yu),최지훈(Ji Hun Choi) 한국식품조리과학회 2017 한국식품조리과학회지 Vol.33 No.4
Purpose: This study was carried out to evaluate the quality characteristics of fermented sausages on the domestic market and to use as a basis data for the development of fermented sausages suitable for domestic consumers. Methods: Out of 13 commercial fermented sausages, four best-selling commercial fermented sausages were chosen. Selected fermented sausages (Romano salami, Iberico salchichon, Italian salami, and Chorizo salami) were analyzed for their physicochemical characteristics (proximate analysis, salinity, pH, water activity, thiobarbituric acid value, volatile basic nitrogen) and counts of lactic acid bacteria. Results: According to the results, water contents and pH were 28.25-40.70% and 4.9-5.1, respectively. Water activity and saltiness were 0.79-0.88 and 2.01-3.96%, respectively. Thiobarbituric acid (TBA) value and volatile basic nitrogen (VBN) were 1.95-3.18 mg/kg and 2.31-3.54 mg%, respectively. Counts of lactic acid bacteria were around 4.3×10<SUP>7</SUP>-1.6×10<SUP>8</SUP> CFU/g. Moreover, the four isolated bacteria were identified as lactic acid bacteria (Lactobacillus plantarum) and color fixing bacteria (Staphylococcus carnosus). For the sensory properties, all products received favorable evaluations due to high-class appearance, common flavor and taste. Conclusion: Fermented sausages on the domestic market had various flavors and qualities due to the different characteristics of materials, fermentation conditions, fat particle size, and so on. Therefore, we need to develop fermented sausages appropriate for Korean consumers by optimizing parameters such as pH and moisture content.
Effects of natural killer cell-derived exosomes in canine mammary tumor model
Se-A Lee,Na-Yeon Gu,So Yeon Jeong,Seong-In Lim,Jienny Lee,Yoon-Hee Lee,Bang-Hun Hyun 한국예방수의학회 2021 예방수의학회지 Vol.45 No.3
Natural killer (NK) cells have cytotoxic effects on tumor cells and viral pathogens. NK cell-derived exosomes (NK-exosomes) also express typical NK cell markers and cytotoxic molecules, therefore, exert anti-tumor and immune homeostatic activities. In this study, canine NK-exosomes separated from cytotoxic NK cell supernatant carried specific markers such as CD81, Alix, and Perforin 1. We examined the anti-tumor effects of NK-exosomes in an experimental murine model using the canine mammary carcinoma cells, REM134. REM134 cells were xenografted of mammary fat pad of mice. CD133, Bmi-1, MMP-3, IL-6, TNF-α, and PCNA are useful as a molecular marker for tumorigenesis and metastasis. The treatment of canine NK-exosomes inhibited tumor growth and significantly (p<0.01) downregulated the expression of Bmi-1, MMP-3, IL-6, TNF-α, and PCNA in REM134-treated mice. Also, the expression of CD133, potent cancer stem cell marker, was significantly downregulated in the canine NK-exosomes-treated mice compared with that of the tumor group. Collectively, these results suggested that canine NK-exosomes has a potential capacity for regulation of cancer progression and metastasis against canine mammary carcinoma.
Se-A Lee,Na-Yeon Gu,Siu Lee,Jienny Lee,Yoon-Hee Lee,Bang-Hun Hyun 한국예방수의학회 2020 예방수의학회지 Vol.44 No.2
Natural killer (NK) cells are innate lymphocytes that aid in the protection of the host from infectious diseases and cancer. Regulation of cytotoxicity in NK cells is mediated by inhibitory receptors that bind major histocompatibility complex class I (MHC-I) molecules on target cells. Human myelogenous leukemia K562 cells are readily attacked by NK cells, because K562 cells expressed very low levels of MHC-I molecules for inhibitory NK cell receptors. In this study, we compared the ability of γ-irradiated- or mitomycin C (MMC)-treated K562 feeder cells to support expansion and activation of canine NK cells. We isolated CD5 negative cells from canine peripheral blood mononuclear cells by immunomagnetic separation and co-cultured with γ-irradiated (100 Gy)- or MMC (20 μg/mL)-treated K562 cells in the presence of interleukin (IL)-2, IL-15 and IL-21 for 21 days. As a result, number of CD5 negative cells, co-cultured with γ-irradiated- or MMC-treated K562 cells (56.72 ± 13.77 fold or 32.99 ± 10.83 fold), was increased than those of CD5 negetive cells (2.99 ± 1.42 fold). Also, we found that gene expression markers of activated NK cells such as NKp30, NKp44, NKp46, Ly49, NKG2D, CD244, perforin, and granzyme B and production of interferon gamma were similarly upregulated in NK cells co-cultured with γ-irradiated- or MMC-treated K562 cells, respectively. Next, we observed that cytotoxicity of NK cells co-cultured with γ-irradiated K562 cells was more sensitively reacted to canine mammary carcinoma cells than those of MMC-treated K562 cells. These results revealed that γ-irradiated K562 cells are more efficient feeder cells than MMC-treated K562 cells for enhancing NK cells expansion and activation.
Undetermined Fibrous Tumor with Calcification in the Cerebellopontine Angle
Cheon, Se-Hun,Kang, Shin-Hyuk,Park, Kyung-Jae,Chung, Yong-Gu The Korean Neurosurgical Society 2010 Journal of Korean neurosurgical society Vol.48 No.2
In this report, we introduce an undetermined fibrous tumor with calcification occurring in the cerebellopontine angle (CPA). A 51-year-old woman was admitted with a short history of dizziness. Computed tomography and magnetic resonance images revealed a $2{\times}2{\times}2\;cm$ sized mass at the left CPA which was round and calcified. There was no dura or internal auditory canal involvement. At surgery, the tumor was located at the exit of 7th and 8th cranial nerve complex. It was very firm, bright yellow and well encapsulated. Histologic findings revealed that the tumor was predominantly composed of fibrous component, scant spindle cells and dystrophic calcification. Immunohistochemical staining demonstrated positive for vimentin and negative for epithelial membrane antigen (EMA), S-100 protein, CD34, factor XIIIa and smooth muscle actin. The diagnosis was not compatible with meningioma, schwannoma, metastatic brain tumors, and other fibrous tumors. Although the tumor was resected in total, long term follow-up monitoring is necessary due to the possibility of recurrence.
인터넷이 정치참여 변화에 미친 영향 : 제15대,16대 대선의 비교 Comparing 16th with 15th Presidential Election
하세헌,강명구 慶北大學校 社會科學大學 2004 社會科學 Vol.16 No.-
인터넷의 사용이 보편화 되면서 정치영역에서 인터넷의 영향에 관한 연구가 이루어졌다. 이들 연구의 대부분은 인터넷의 영향으로 정치참여가 확대된 것으로 나타났는데 이러한 연구 결과에 의문을 가지고 새로운 방법으로 인터넷의 정치참여에 대한 영향을 확인해 보고자 하였다. 본 연구는 한국사회과학데이터센터가 수집한 지난 제15, 16대 대선 직후 실시한 전국데이터를 이용하여 정치참여 특히 투표참여에 영향을 미치는 요인인 정치효능감, 선거관심, 선거공정성의 변화를 평균비교분석(T-Test)을 이용하여 분석하였다. 분석 결과 인터넷의 역할이 거의 없었던 15대 대선에 비해 인터넷의 사용이 보편화된 16대 대선의 정치효능감, 선거관심, 선거공정성이 오히려 줄어들거나 변화가 없는 것으로 나타났다. 이러한 결과는 인터넷의 주 사용 층인 20, 30대 유권자의 경우에도 마찬가지로 나타났다. 이것은 새로운 매체인 인터넷이 일상적인 영역에서는 활용도가 높은 반면 정치적 목적으로의 활용도가 낮기 때문인 것으로 보인다. 인터넷을 정치적 목적으로 사용하는 사람들의 경우는 정치적 관심이 높은 사람들인 것으로 나타났다. 즉 인터넷이 정치적 무관심자 층을 정치적 관심자 층으로 끌어들이는 것이 아니라, 정치적 관심자의 욕구를 충족시키는 역할만을 하는 것으로 나타났다. This paper contradicted to general opinion about the role of Internet upon political participation. Many scholars say that the spread of Internet use makes grow political participation. They verified their claims with various way but that had something wrongs and I could not trust their conclusions. So this thesis tried different way to analyze the influence of Internet. It was hard to find out causal relation between Internet and political participation directly. With this reason, this thesis compared a difference of the feel efficacy, fairness and interest at the 16th with 15th presidential election. As the result from the statistical data through SPSS, the feel of efficacy, fairness and interest of 16th electorate were lower than 15th. Then, Why using Internet could not make enlarge the political participation even though it has many advantages? The cause was that most of the Internet users didn't use Internet for political purpose. In addition, the people who obtained political information from Internet had been interested in political affairs. In conclusion, the spread of Internet use does not make enlarge the political participation. It just make deeper one's interest who has interest in political affairs already.