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Establishment of a Stable Amaranthus tricolor Callus Line for Production of Food Colorant
Mousumi Biswas,Shibendu Sekhar Das,Satyahari Dey 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.suppl1
The present study attempts to establish and screen Amaranthus tricolor L. callus for development of a stable callus line with enhanced production of food colorants. The best betacyanin-producing callus was induced on Murashige and Skoog basal medium supplemented with 1-naphthalene acetic acid (NAA) (0.25 mg/L) and 6-benzylaminopurine (BA) (2 mg/L) using stem segment as explants. The callus line (IIT BT/PBT At 11) was maintained up to 24 subculture cycles. Betalain production in callus was detected by reversed phase HPLC and LCMS. In addition to the reported abundant red-violet pigment amaranthin, 2 new yellow pigments and 18 other bioactive phenylpropanoids were detected in the callus line. A novel betaxanthin, methyl derivative of arginine betaxanthin was identified. All pigments were purified by size exclusion chromatography.