http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Saptadip Samanta (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Chiranjit Maity,Saptadip Samanta,Suman K. Halder,Pradeep K. Das Mohapatra,Bikas R. Pati,Malabendu Jana,Keshab C. Mondal 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.2
The aim of this study was to produce two isozymes of α-amylase by immobilization of a newly isolated soil bacterium. The bacterium was identified as Bacillus thuringiensis CKB19 on the basis of its 16S rRNA profile. Enzyme production by free cells increased linearly with cell growth up to 34 h in starch containing enriched liquid media. The active bacterial cells were immobilized in Caalginate beads, and operational stability of the entrapped cell was optimized for amylase production. Enzyme production was optimal at an alginate concentration of 2 g%(w/v), calcium chloride concentration of 1 M, and with 300beads (each bead contained 2 × 10^7 cells)/250 mL flask. Amylase production by the immobilized cells was about 3times higher than free cell fermentation after 34 h of incubation. It was observed that the immobilized bacterium secreted two different amylases (Am-I and Am-II) into the culture fluid. The molecular masses of Am-I and Am-II were 59.6 and 44.7 kd, respectively, and showed optimum activity at pH 5.0 and 9.0. Both amylases showed optimum activity at 40℃ and were stable at the same temperature,with losses of only 10 and 20% (for Am I and Am II,respectively) of their original activities after 24 h of incubation. Further, both amylases were salt tolerant (up to 4 M NaCl) and hydrolyzed raw starchy foods into glucose. All these characteristics make this enzyme mixture suitable for use as a digestive aid and for the improvement of digestibility of animal feed ingredients.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
