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RISS 인기검색어
- 검색키워드
- 저자 : Rintu Banerjee (검색결과 4 건)
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Sangeeta Negi,Rintu Banerjee 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.1
Various parameters such as solvent selection, concentration, soaking time, and temperature were tested in a single bioreactor in order to determine optimum extraction conditions of glucoamylase, when produced simultaneously with protease by Aspergillus awamari nakazawa MTCC 6652. Optimum conditions were achieved in a 10% glycerol solution soaked for 2 h at 40℃, followed by concentration of extracted glucoamylase (9,157 U/gds) by acetone precipitation (1:2, v/v), which yielded 51.9% recovery. Ion exchange chromatography and gel filtration showed specific activities of 270.5 and 337.5 U/mg, respectively, while SDS-PAGE and zymogram analysis of glucoamylase indicated the presence of three starch-hydrolyzing isoforms with molecular weights of approximately 109.6, 87.1, and 59.4 kDa, respectively.
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Peptide Enriched Functional Food Adjunct from Soy Whey: A Statistical Optimization Study
Anshu Singh,Rintu Banerjee 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.suppl1
Soy whey is generated as a process waste while preparing soy based food products tofu, causing environmental pollution and also representing an economic penalty against the industrial process. Therefore, its valorization is of prime importance to the industry. The present investigation aims to convert this proteinaceous waste into bioactive peptide enriched hydrolysate. Soy whey protein was enzymatically treated with the Aspergillus awamori nakazawa protease. Respective protease was efficient to produce antioxidant peptide beholding radical scavenging ability of 40-50% at normal conditions. Remarkable increase in the radical scavenging activity upto 70% was noticed at the response surface methodology (RSM) based optimized condition: temperature 40oC, salt concentration (NaCl) 0.05 M, surfactant concentration (Triton-X 100) 0.0075%, hydrolysis time 80 min, and enzyme to substrate concentration 164 IU/g of soy whey protein. The present study emphasizes the biotransformation of proteineceous waste into antioxidant peptide rich soy whey protein hydrolysate to be considered as additives for food preparation and formulation.
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Optimization of Lipase Production using Differential Evolution
Vijay Kumar Garlapati,Rintu Banerjee 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.2
Differential Evolution (DE) coupled with Response Surface Methodology (RSM) has been used for the optimization of extracellular lipolytic enzyme production by Rhizopus oryzae NRRL 3562 through sold state fermentation. The input space of the experimentally validated RSM-model was optimized using a novel Differential Evolution approach, which works based on the natural selection and survival of the fittest concepts of the biological world. The maximum lipase activity of 96.52 U/gds was observed with the DE stated optimum values of 35.59oC, 1.50, 5.28, and 4.83 days for temperature, liquid to solid ratio, pH, and incubation time respectively. The optimal levels of control parameters namely number of population, generations, crossover operator, and mutation constant were equal to 20, 50, 0.6, and 0.20, respectively. The developed model and its optimization are generic in nature and thus appear to be useful for the design and scale-up of the extracellular lipase production by R. oryzae NRRL 3562 through solid state fermentation.
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Moumita Bishai,Swarnalok De,Basudam Adhikari,Rintu Banerjee 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.suppl1
The present study involves synthesis of polylactic acid (PLA) using purified lactic acid from fermented broth of Jackal jujube (Zizyphus oenophlia). A polyphenolic compound, humic acid (HA) of biological origin was incorporated to the PLA in order to reinforce the PLA chain without compromising its biodegradability and biocompatibility. Under optimized conditions of polymerization,modified L-PLA yield improved up to 93%. The molecular weight was found to be 6.4×105. Different physicochemical properties of the polymer were explored for its further application in different fields. Incorporation of intermolecular bond between PLA and HA was confirmed by FT-IR spectroscopy technique. Addition of HA not only reduced the crystallinity of PLA, but also had increased flexibility and elasticity to much greater extent. The results showed that, apart from enhancing the physicochemical properties of PLA, the process also had reduced the production cost of the polymer, while mitigating the demands of environmental protection agencies.
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