http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Rimenys Junior Carvalho (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Comparison of Cationic Flocculants for the Clarification of CHO-derived Monoclonal Antibodies
Rimenys Junior Carvalho 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.5
This work used two different cationic polyelectrolytes as flocculation agents for the clarification of growth medium from Chinese hamster ovary (CHO) monoclonal antibodies (mAb)-producing cells, namely chitosan and poly diallyldimethylammonium chloride (pDADMAC). The conditions set for the use of pDADMAC consisted in the variation of the polymer’s concentrations, in light of largely available literature. Chitosan was applied to a broader concentration-range under two different pH values, for its use in CHO cells flocculation is unprecedented in the literature. Chitosan presented the best performance on clarification assays under a concentration 9-fold lower compared with pDADMAC (5 pg/total cell (TC) and 45 pg/TC, respectively), albeit both agents provided similar results regarding sedimentation rates (15min), DNA removal (98%), and mAb recovery (90%). Despite the expected low proteins removal, namely 11.1% in the case of chitosan and no removal for pDADMAC, the flocculation technique proved to be cost-effective. Hence, its application can be envisioned not only when the clarification of a growth medium from CHO cells production is considered, but also for the removal of soluble impurities in a single step.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
