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Expression and Functional Role of ALDH1 in Cervical Carcinoma Cells
Rao, Qun-Xian,Yao, Ting-Ting,Zhang, Bing-Zhong,Lin, Rong-Chun,Chen, Zhi-Liao,Zhou, Hui,Wang, Li-Juan,Lu, Huai-Wu,Chen, Qin,Di, Na,Lin, Zhong-Qiu Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
Tumor formation and growth is dictated by a very small number of tumor cells, called cancer stem cells, which are capable of self-renewal. The genesis of cancer stem cells and their resistance to conventional chemotherapy and radiotherapy via mechanisms such as multidrug resistance, quiescence, enhanced DNA repair abilities and anti-apoptotic mechanisms, make it imperative to develop methods to identify and use these cells as diagnostic or therapeutic targets. Aldehyde dehydrogenase 1 (ALDH1) is used as a cancer stem cell marker. In this study, we evaluated ALDH1 expression in CaSki, HeLa and SiHa cervical cancer cells using the Aldefluor method to isolate ALDH1-positive cells. We showed that higher ALDH1 expression correlated with significantly higher rates of cell proliferation, microsphere formation and migration. We also could demonstrate that SiHa-ALDH1-positive cells were significantly more tumorigenic compared to SiHa-ALDH1-negative cells. Similarly, SiHa cells overexpressing ALDH1 were significantly more tumorigenic and showed higher rates of cell proliferation and migration compared to SiHa cells where ALDH1 expression was knocked down using a lentivirus vector. Our data suggested that ALDH1 is a marker of cervical cancer stem cells and expand our understanding of its functional role.
WANGPING RAO,JIANXI YAO,SONGYUAN DAI,BING ZHANG,YONGZHE ZHANG,LI XIAO 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.5
We demonstrate a strategy for incorporating plasmon resonant metallic nanoparticles in theconstruction of the three-dimensional (3D) interwoven structured TiO 2 photoanodes. The 3Dinterwoven structure contained continuous TiO 2 skeleton and numerous interconnected macro/mesopores, which supplied e®ective straight path for electron transfer and high speci¯c surfacearea for dye load. Localized surface plasmon resonance (SPR) was produced by the addition ofgold nanoparticles (AuNPs), which increased the light absorption of the photoanodes moree®ectively. The synergistic e®ect of SPR with constructed TiO 2 nanostructures has been in-vestigated, and was con¯rmed by ¯eld-emission scanning electron microscope (FESEM), high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy(XPS), UV-Vis absorption spectra, J – V characteristics, and electrochemical impedance spec-troscopy (EIS) analysis. It was found that the range and strength of light absorption of TiO 2photoanodes, the photon capture ability of the dye molecules, the photoelectric conversion e±-ciency were signi¯cantly increased while the electron transfer resistance decreased due to theincorporation of AuNPs compared to the P25 and Au-free photoanode. The related photoelectricperformance enhancement mechanisms, and surface-plasmon resonances in dye-sensitized solarcells (DSSCs) with Au nanostructures were analyzed and discussed.